Table I
Phylogenetic analysis of petrol metabolizing bacteria.
| Group | Isolate | Accession No. | Closest phylogenetic relative | Similarity | URLs |
|---|---|---|---|---|---|
| Group I | IUBP2 | MH023312 | Brevibacillus formosus strain NBRC 15716 | 100% | https://www.ncbi.nlm.nih.gov/nuccore/MH023312.1 |
| IUBP3 | MH023313 | Brevibacillus formosus strain NBRC 15716 | 100% | https://www.ncbi.nlm.nih.gov/nuccore/MH023313.1 | |
| IUBP5 | MH023314 | Brevibacillus formosus strain NBRC 15716 | 100% | https://www.ncbi.nlm.nih.gov/nuccore/MH023314.1 | |
| Group II | IUBP1 | MH368051 | Brevibacillus agri strain NBRC 15538 | 100% | https://www.ncbi.nlm.nih.gov/nuccore/MH368051 |
| Group III | IUBP7 | MH368052 | Burkholderia lata strain 383 | 99% | https://www.ncbi.nlm.nih.gov/nuccore/MH368052.1 |
| IUBP8 | MH368053 | Burkholderia lata strain 383 | 99% | https://www.ncbi.nlm.nih.gov/nuccore/MH368053.1 | |
| IUBP13 | MH368054 | Burkholderia lata strain 383 | 99% | https://www.ncbi.nlm.nih.gov/nuccore/MH368054.1 | |
| IUBP14 | MH368055 | Burkholderia lata strain 383 | 99% | https://www.ncbi.nlm.nih.gov/nuccore/MH368055.1 | |
| Group IV | IUBP15 | MH368057 | Burkholderia pyrrocinia strain LMG 14191 | 98% | https://www.ncbi.nlm.nih.gov/nuccore/MH368057 |

Fig. 1.
Characteristics of petrol metabolizing bacteria based upon molecular, biochemical and growth curve analysis. The ribotyping, biochemical, growth behavior analysis helped to discriminate among the bacteria isolated.

Fig. 2.
A) Phylogenetic tree of petrol metabolizing bacteria exhibiting homology with Burkholderia. B) Phylogenetic tree of petrol metabolizing bacteria exhibiting homology with Brevibacillus.