Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway Cover

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Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway

Oceanological and Hydrobiological Studies

Volume 52 (2023): Issue 2 (June 2023)

By: Muhammed Atamanalp, Muammer Kırıcı, Mine Köktürk, Mahinur Kırıcı, Duried Alwazeer, Esat Mahmut Kocaman, Arzu Ucar, Veysel Parlak, Sinan Özcan and Gonca Alak

Open Access

|Jun 2023

Figures & Tables

Microscope images of polyethylene microplastics used in dietary exposure of O. mykiss and size distribution of polyethylene microplastics (PE MPs) analysed through particle counting

Microscope images of polypropylene microplastics used in dietary exposure of O. mykiss and size distribution of polypropylene microplastics (PP MPs) analysed through particle counting

(A-B) FTIR spectra of microplastics used in dietary exposure of O. mykiss

Microscopic images of PE and PP MPs in the tissues of dieted fish

Antioxidant enzyme activity of fish tissues after the application of different recovery treatments. Lowercase superscripts (a, b, c) indicate significant differences among the same colon within each experimental treatment group, *p < 0.05

Antioxidant and oxidation status of fish tissues after the application of different recovery treatments. Lowercase superscripts (a, b, c) indicate significant differences among the same colon within each experimental treatment group, *p < 0.05

DNA damage level and apoptosis activity of fish tissues after the application of different recovery treatments. Lowercase superscripts (a, b, c) indicate significant differences among the same colon within each experimental treatment group, *p < 0.05

AChE activity after the application of different recovery treatments. Lowercase superscripts (a, b, c) indicate significant differences among the same colon within each experimental treatment group, *p < 0.05

Amount, dimensions, and polymer type of PP and PE MPs in muscle, liver, gill, and gastrointestinal tract tissues of O_ mykiss after dietary exposure

Fish tissues (n=5)	Treatment groups
	Control			HRW			PP+PE			PP+PE+HRW
	Average MPs	Size range (μm)	Microplastic type (%)	Average MPs	Size range (μm)	Microplastic type (%)	Average MPs	Size range (μm)	Microplastic type (%)	Average MPs	Size range	Microplastic type (%)
Muscle (MPs g^-1)	0	-	-	0	-	-	0.2 ±0.4	0-40	100% PP	0	-	-
Liver (MPs ind.^-1)	0	-	-	0	-	-	0.4 ±0.5	41-51	100%PP	0	-	-
Gills (MPs ind.^-1)	0.8 ± 0.4	129-229	75% PE, 25% PP	0.6 ±0.5	111-255	66.7% PP, 33.3% PE	3.8 ±1.3	44-255	52.6% PE, 47.4% PP	3.2 ±0.8	66-200	56.3% PE, 43.8% PP
Gastrointestinal system (MPs ind.^-1)	0.4 ± 0.5	92-107	%50 PE, %50 PP	0.8 ±0.4	95-274	75% PP, 25% PE	3.8 ±0.8	67-325	63.2% PE, 36.8% PP	1.2 ±0.8	125-279	66.7% PE, 33.3 %PP

Hematological index alterations

Hematological index	Treatment groups	Values (Mean ± SD)
Hemoglobin (g dL)	Control	9.3 ± 0.3^b
	PP+PE	8.5 ± 0.3^b
	PP+PE+HRW	11.9 ± 0.8^a
	HRW	9.0 ± 0.02 ^b
RBC (10⁶ mm^-3)	Control	1.3 ±0.1^b
	PP+PE	1.1 ± 0.04^c
	PP+PE+HRW	1.8 ± 0.08^a
	HRW	1.0 ± 0.1^d
WBC (10⁴ mm^-3)	Control	13.9 ± 1.7^b
	PP+PE	9.8 ± 0.9^c
	PP+PE+HRW	16.7 ± 0.9^a
	HRW	9.6 ± 0.6 ^c
PLT (10⁴ mm^-3)	Control	445.7 ± 26.1^a
	PP+PE	357.7 ± 13.7^ab
	PP+PE+HRW	545.3 ± 22.7^a
	HRW	248.0 ± 98.8^b
HCT (%)	Control	34.1 ± 1.3^b
	PP+PE	29.0 ± 1.7^c
	PP+PE+HRW	37.9 ± 0.4^a
	HRW	34.3 ± 1.15 ^b
MCV (μm^-3)	Control	157.7 ± 4.3^b
	PP+PE	155.3 ± 6.2^b
	PP+PE+HRW	157.5 ± 6.4^b
	HRW	284.3 ± 14.2 ^a
MCH (pg)	Control	54.1 ± 2.1^b
	PP+PE	52.7 ± 0.6^b
	PP+PE+HRW	52.5 ± 2.1^b
	HRW	74.9 ± 4.3^a
MCHC (g 100 ml^-1)	Control	27.3 ± 1.5^bc
	PP+PE	29.3 ± 1.7^ab
	PP+PE+HRW	31.3 ± 1.9^a
	HRW	26.23 ± 0.4^c

Total length, total weight, tissue and organ weights of the fish used in the experimental groups

Groups (n = 15)	Total weight (g)	Total length (cm)	Liver (g)	Gills (g)	Gastrointestinal system (g)	Muscle (g)
Control	207.5 ±54.6	24.8±1.0	3.5±0.5	5.1±0.8	18.2±0.6	^~5
HRW	267.2 ±54.6	27.2±1.3	3.8±0.3	6.2±0.7	20.7±4.0	^~5
PP+PE	235.0 ±86.8	26.6±2.6	3.6±0.6	4.9±1.0	17.6±3.3	^~5
PP+PE+HRW	159.5 ±15.2	23.0±0.6	2.5±0.4	3.7±0.3	16.2±2.5	^~5

DOI: https://doi.org/10.26881/oahs-2023.2.05 | Journal eISSN: 1897-3191 | Journal ISSN: 1730-413X

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Language: English

Page range: 206 - 220

Submitted on: Mar 29, 2023

Accepted on: Apr 21, 2023

Published on: Jun 28, 2023

Published by: University of Gdańsk

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Oxy-hydrogen gas,

hydrogen-rich water,

molecular hydrogen,

antioxidant status,

Related subjects:

Chemistry, other,

Geosciences, other,

Life sciences, other

© 2023 Muhammed Atamanalp, Muammer Kırıcı, Mine Köktürk, Mahinur Kırıcı, Duried Alwazeer, Esat Mahmut Kocaman, Arzu Ucar, Veysel Parlak, Sinan Özcan, Gonca Alak, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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