Molecular identification and distribution of insect larvae in the Lower Danube River

Selma Menabit; Lavinia Iancu; Ana B. Pavel; Adrian Popa; Naliana Lupascu; Cristina Purcarea

doi:10.26881/oahs-2022.1.07

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Molecular identification and distribution of insect larvae in the Lower Danube River

Oceanological and Hydrobiological Studies

Volume 51 (2022): Issue 1 (March 2022)

By: Selma Menabit, Lavinia Iancu, Ana B. Pavel, Adrian Popa, Naliana Lupascu and Cristina Purcarea

Open Access

|Mar 2022

Figures & Tables

Map of the sampling areas in the Lower Danube River (A) and the Danube Delta (B)

Densities and distribution of insect larvae, depth (m) and oxygen records in the Lower Danube River

Year of sampling, depth, substrate type, dissolved oxygen and presence of macrophytes

Crt. No.	Sites	Year of sampling	Depth (m)	Type of substrate	Dissolved oxygen mg/L		Macrophytes presence (+/-)
Crt. No.	Sites	Year of sampling	Depth (m)	Type of substrate	Mean values/area	Value/site	Macrophytes presence (+/-)
Corabia area					8.58
1.	km 626	2019	3.0	sand		8.90	-
2.	km 626.5	2020	7.3	gravelly sand		8.10	-
3.	km 627.5	2020	5.0	sand		8.81	-
4.	km 629	2019	9.2	gravelly sand		8.82	-
5.	km 630	2019	8.2	gravelly sand		8.04	+
5.	km 630	2020	8.2	gravelly sand		8.67	+
6.	km 631	2019	5.3	sand		8.77	-
Bechet area					8.77
7.	km 674	2019	3.3	sand		8.75	-
8.	km 674.5	2019	3.2	sand		8.75	+
9.	km 675	2019	4.0	sand		8.67	-
10.	km 675.5	2019	6.3	sand		8.67	+
11.	km 676	2019	3.7	gravelly sand		9.31	+
11.	km 676	2020	3.7	gravelly sand		8.10	+
12.	km 676.5	2020	3.0	sand		8.15	-
13.	km 678	2019	5.9	sand		9.78	+
Pisculeţ area					8.85
14.	km 760	2019	3.9	sand		9.36	-
15.	km 761	2019	4.2	sand		9.61	+
16.	km 762	2019	5.0	sand		8.95	+
16.	km 762	2020	5.0	sand		8.99	+
17.	km 762.5	2019	4.8	gravelly sand		8.96	-
18.	km 764	2019	3.5	sand		8.55	+
19.	km 764.5	2019	4.9	sand		8.55	+
20.	km 765	2019	2.8	gravelly sand		8.38	+
20.	km 765	2020	2.8	gravelly sand		8.31	+
Cetate area					7.80
21.	km 806	2019	3.8	gravelly sand		8.13	-
22.	km 808	2019	5.0	sand		7.25	-
23.	km 811	2019	7.8	gravelly sand		8.02	-
		Sulina area			8.25
24.	Old Danube meander	2019	5.0	sandy mud		7.04	-
25.	NM 24(a) Sulina branch	2019	16.5	sand		7.93	-
26.	NM 16 Sulina branch	2019	5.2	muddy sandy gravel		7.90	-
27.	NM 8(a) Sulina branch	2019	14.4	sandy mud		7.95	-
28.	NM 2 Sulina branch	2019	17.5	gravelly mud		7.96	-
29.	NM 33 Sulina branch	2019	12.5	gravelly mud		9.38	-
30.	NM 24(b) Sulina branch	2019	12.5	sand		9.01	-
31.	NM 14(b) Sulina branch	2019	13.5	sandy mud		9.55	-
32.	NM 8(b) Sulina branch (Meander)	2019	4.3	sandy mud		9.07	-
33.	NM 14(c) Sulina branch (Crisan)	2019	11.5	sand		7.95	-
34.	NM 14(a) Sulina branch (Crisan)	2019	10.7	gravelly mud		7.95	-
35.	NM 21 Sulina channel	2019	6.5	sandy mud		7.97	-
36.	Km 108(a) Ceatal Sf. Gheorghe	2019	6.4	sandy mud		7.97	-
37.	Km 108(b) Ceatal Sf. Gheorghe	2019	6.4	gravelly mud		7.97	-

Sampling locations in the Lower Danube and density of larvae

Crt. No.	Sites	Coordinates		Number of individuals/m²
Crt. No.	Sites	Lat. (α)	Long. (λ)	G.f.	H.f.	E.v.	A.e.	H.b.	T.b.	S.m.
1.	* km 811	44°4’53.2”	23°1’48.8”	0	0	7.4			0	0
2.	** km 808	44°3’49.7”	23°3’7.4”	0	0	0	0	50.4	0	0
3.	** km 806	44°2’55.3”	23°2’14.5”	0	0	0	0	226.8	0	0
4.	** km 765	43°48’18.2”	22°57’34.3”	0	0	0	0	403.2	0	0
5.	** km 765	43°48’18.2”	22°57’34.3”	0	0	0	0	0	25.2	0
6.	** km 764.5	43°48’36.8”	22°58’12.0”	0	0	0	0	0	1008	0
7.	** km 764	43°48’5.7”	22°58’35.5”	0	0	0	0	25.2	25.2	0
8.	* km 762.5	43°48’37.7”	22°59’52.7”	7.4	0	0	0	162.8		0
9.	* km 762	43°47’54.0”	23°0’2.4”	0	29.6	0	0	0	0	0
10.	* km 762	43°47’54.0”	23°0’2.4”	0	0	0	0	4388.2	7.4	0
11.	* km 761	43°48’14.3”	23°0’54.4”	0	0	0	0	0	7.4	0
12.	** km 760	43°48’9.8”	23°1’51.1”	0	0	0	0	201.6	0	0
13.	* km 678	43°44’40,6”	23°57’52.9”	0	0	7.4	0	7.4	0	0
14.	* km 676.5	43°44’18.0”	23°58’42.9”	0	0	0	0	96.2	0	0
15.	* km 676	43°44’13.0”	23°59’4.3”	0	0	0		1813	0	0
16.	* km 676	43°44’13.0”	23°59’4.3”	0	0	0	29.6	0	0	0
17.	* km 675.5	43°44’38,7”	23°59’42,9”	0	7.4	0	0	7.4	7.4	0
18.	* km 675	43°44’5.5”	23°59’45.1”	0	0	7.4	0	0	0	0
19.	* km 674.5	43°44’3.0”	24°0’8.1”	0	66.6	0	7.4	488.4	0	0
20.	** km 674	43°43’52.9”	24°0’27.3”	0	0	0	0	25.2	0	0
21.	* km 631	43°46’8.3”	24°29’58.8”	0	96.2	0	0	0	0	0
22.	* km 630	43°45’32.0”	24°30’49.5”	0	429.2	14.8	0	0	0	0
23.	* km 630	43°45’32.0”	24°30’49.5”	0	0	0	0	1894.4	0	0
24.	* km 629	43°45’25.4”	24°31’18.5”	0	0	0	0	7.4	0	0
25.	** km 627.5	43°45’17.2”	24°32’34.2”	0	25.2	0	0	252	0	0
26.	** km 626.5	43°45’46.6”	24°33’30.4”	0	0	0	0	100.8	0	0
27.	* km 626	43°45’37.5”	24°33’48.9”	29.6	7.4	0	0	310.8	0	0
28.	* km 108(a) Ceatal Sf. Gheorghe	45°11’04.9”	28°53’26.7”	0	0	0	0	214.6	0	0
29.	* km 108(b) Ceatal Sf. Ghoerghe	45°11’04.39”	28°53’26.7”	0	0	0	0	7.4	0	0
30.	* NM 33 Sulina branch	45°11’22.8”	28°55’22.3”	0	0	0	0	74	0	0
31.	* NM 24 (a) Sulina branch	45°10’28.5”	29°03’19.4”	0	0	0	0	384.8	0	0
32.	* NM 24 (b) Sulina branch	45°10’29.9”	29°03’19.3”	0	0	0	0	629	0	0
33.	* NM 21 Sulina channel	45⁰10’54.2”	29°10’19.1”	0	0	0	0	1420.8	0	0
34.	* NM 16 Sulina branch	45°10’33.3”	29°19’05.5”	0	0	0	0	651.2	0	0
35.	* NM 14 (a) Sulina branch	45°10’33.9”	29°21’34.8”	0	0	0	0	370	0	0
36.	* NM14 (b) Sulina branch	45°10’32.4”	29°21’17.0”	0	0	0	0	1206.2	0	0
37.	* NM 14 (c) Sulina branch (Crisan)	45°10’30.9”	29°21’39.6”	0	0	0	0	518	0	0
38.	* Old Danube (Meander)	45°10’41.4”	29°21’05.1”	0	0	0	0	0	7.4	0
39.	* NM 8 (a) Sulina branch	45°10’20.3”	29°28’47.6”	0	0	0	0	148	0	0
40.	* NM 8 (b) Sulina branch	45°10’39.4”	29°28’33.2”	0	0	0	0	0	0	14.8
41.	* NM 2 Sulina branch	45°09’39.6”	29°36’31.6”	0	0	0	0	296	0	0

Best match COI gene sequence of insect larvae species from the Danube River

Insect species	Identity (%)	Cover (%)	GenBank reference
E. viridulum	99.84	100.00	MT298455.1
G. flavipes	99.36	98.00	MT298633.1
T. bicolor	99.83	99.00	KX143573.1
H. bulgaromanorum	100.00	98.00	KX104210.1
H. flava	99.84	98.00	KY262534.1
A. ephemerella	98.66	97.00	LR135742.1
S. morio	98.41	97.00	JX438311.1

Association of insect larvae with different types of substrate

Insect species	Order	Type of substrate
Insect species	Order	Sand	Gravelly sand	Muddy sandy gravel	Gravelly mud	Sandy mud
Erythromma viridulum	Odonata	+	+	-	-	-
Gomphus flavipes	Odonata	+	+	-	-	-
Triaenodes bicolor		+	+	-	-	+
Hydropsyche bulgaromanorum	Trichoptera	+	+	+	+	+
Heptagenia flava	Ephemeroptera	+	+	-	-	-
Acentria ephemerella	Lepidoptera	+	+	-	-	-
Sialis morio	Megaloptera	-	-	-	-	+

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DOI: https://doi.org/10.26881/oahs-2022.1.07 | Journal eISSN: 1897-3191 | Journal ISSN: 1730-413X

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Language: English

Page range: 74 - 89

Submitted on: Jul 15, 2021

Accepted on: Nov 18, 2021

Published on: Mar 31, 2022

Published by: University of Gdańsk

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

aquatic insect larvae distribution,

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© 2022 Selma Menabit, Lavinia Iancu, Ana B. Pavel, Adrian Popa, Naliana Lupascu, Cristina Purcarea, published by University of Gdańsk
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 51 (2022): Issue 1 (March 2022)

Molecular identification and distribution of insect larvae in the Lower Danube River

Figures & Tables

Figure 1

Figure 2

Figure 2

Year of sampling, depth, substrate type, dissolved oxygen and presence of macrophytes

Sampling locations in the Lower Danube and density of larvae

Best match COI gene sequence of insect larvae species from the Danube River

Association of insect larvae with different types of substrate

Paradigm

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