Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy

Anna Rzepecka-Stojko; Barbara Pilawa; Paweł Ramos; Jerzy Stojko

doi:10.2478/v10289-012-0003-0

.blurhash-client-img { display: none !important; }

Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy

Journal of Apicultural Science

Volume 56 (2012): Issue 1 (June 2012)

By: Anna Rzepecka-Stojko, Barbara Pilawa, Paweł Ramos and Jerzy Stojko

Open Access

|Jun 2012

Abstract

Bee pollen is a valuable and highly recognized source of exogenous antioxidants. The aim of these studies was to determine the antioxidant capacity of three types of bee pollen extracts: ethanol extracts of bee pollen, pepsin extracts of bee pollen and ethanol extracts of pepsin-digested bee pollen. Their antioxidant properties were determined with the use of electron paramagnetic resonance (EPR) and their ability to quench DPPH free radicals was estimated. The EPR results showed that ethanol extracts of pepsin-digested bee pollen (EEPP) had the highest antioxidative effect and the highest free radical DPPH scavenging potential. The pepsin extracts of bee pollen (PEP) had the weakest antioxidant capacity. The ability to quench DPPH free radicals was also the weakest one for this extract. An average antioxidative effect was recorded for ethanol extracts of bee pollen (EEP).

References

Almaraz-Abarca N., Campos M. G., Avila-Reyes J. A., Naranjo-Jimenez N., Carrol H. J., Gonzalez-Valdez L. S. (2007) - Antioxidant activity of polyphenolic extract of monofloral honeybee-collected pollen from mesquite (Prosopis juliflora, Leguminosae). J. Food Compos. Anal., 20(2): 119-124.10.1016/j.jfca.2006.08.001
Search in Google Scholar
Baltrusaityte V., Venscutonis P. R., Ceksteryte V. (2007) - Radical scavenging activity of different floral origin honey and beebread phenolic exreacts. Food Chem., 101(2): 502-514.10.1016/j.foodchem.2006.02.007
Search in Google Scholar
Bartosz G. (2005) - Druga twarz tlenu. Wolne rodniki w przyrodzie. Wydawnictwo Naukowe PWN, Warszawa.
Search in Google Scholar
Campos M. G., Webby R. F., Markham K. R., Mitchell K. A., Cuhna A. P. (2003) - Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids. J. Agric. Food Chem., 51: 742-745.
Search in Google Scholar
Carpes S. T., Mourao G. B., Alencar S. M., Masson M. L. (2009) - Chemical composition and free radical scavenging activity of Apis mellifera bee pollen from Southern Brazil. Braz. J. Food Technol., 12(3): 220-229.10.4260/BJFT2009800900016
Search in Google Scholar
Ceksteryte V., Racys J., Kaskoniene V., Venskutonis P. R. (2008) - Fatty acid composition in beebread. Biologija, 54(4): 253-257.10.2478/v10054-008-0052-2
Search in Google Scholar
Gomez-Caravaca A. M., Gomez-Romero M., Arraez-Roman D., Segura-Carretero A., Fernandez-Gutierrez A. (2006) - Advances In the analysis of phenolic compounds In products derived from bees. J. Pharm. Biomed. Anal., 41:1220-1234.
Search in Google Scholar
Grajka W. ed. (2007) - Przeciwutleniacze w żywności. Aspekty zdrowotne, technologiczne, molekularne i analityczne. Wydawnictwa Naukowo-Techniczne, Warszawa, pp. 538.
Search in Google Scholar
Jóźwiak Z., Bartosz G. (2005) - Biofizyka. Wybrane zagadnienia. Wydawnictwo Naukowe PWN, Warszawa, pp.
Search in Google Scholar
Kroyer G., Hegedus N. (2001) - Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innov. Food Sci. Emerg. Technol., 2: 171-174.
Search in Google Scholar
Kęcki Z. (1992) - Podstawy spektroskopii molekularnej. Wydawnictwo Naukowe PWN, Warszawa, pp. 297-322.
Search in Google Scholar
LeBlanc B. W., Davis O. K., Boue S., DeLucca A., Debby T. (2009) - Antioxidativ activity of Sonorant Desert bee pollen. Food Chem., 115: 1299-1305.
Search in Google Scholar
Leja M., Mareczek A., Wyżgolik G., Klepacz-Baniak J., Czekońska K. (2007) - Antioxidative properties of bee pollen in selected plant species. Food Chem., 100: 237-240.
Search in Google Scholar
Marghitas L. A., Stanciu O. G., Dezmirean D. S., Bobis O., Popescu O., Bogdanov S., Campos, M. G. (2009) - In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem., 115: 878-883.
Search in Google Scholar
Nagai T., Inoue R., Suzuki, N., Myoda, T., Nagashima T. (2005) - Antioxidative ability in a linoleic acid oxidation system and scavenging abilities against active oxygen species of enzymatic hydrolysates from pollen Cistus ladaniferus. Int. J. Mol. Med., 15: 259-263.10.3892/ijmm.15.2.259
Search in Google Scholar
Rzepecka-Stojko A., Maciejewska-Paszek I., Stec M., Kurzeja E., Kęska A., Pawłowska-Góral K. (2010) - The influence of extraction method on obtaining polyphenolic compounds from bee pollen. Farm. Przegl. Nauk., 1: 38-41.
Search in Google Scholar
Saric A., Balog T., Sobocanec S., Kusic B., Svarko V., Rusac G., Likic S., Bubalo D., Pinto B., Reali D., Maritti T. (2009) - Antioxidant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem. Toxicol., 47: 547-554.
Search in Google Scholar
Stankowski J., Hilczer W. (1994) - Pierwszy krok ku radiospektroskopii rezonansów magnetycznych. OWN, Poznań, pp. 54-71.
Search in Google Scholar
Szczęsna T. (2006) - Protein content and amino acid composition of bee-collected pollen from selected botanical origins. J. Apic. Sci., 50(2): 81-90.
Search in Google Scholar
Wertz J. E., Bolton, J. R. (1986) - Electron spin resonance. Elementary theory and practical applications. McGraw-Hill, New York, London, pp. 154-176.10.1007/978-94-009-4075-8
Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.2478/v10289-012-0003-0 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439

Journal RSS Feed

Language: English

Page range: 23 - 31

Published on: Jun 19, 2012

Published by: Research Institute of Horticulture

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

Related subjects:

Zoology,

© 2012 Anna Rzepecka-Stojko, Barbara Pilawa, Paweł Ramos, Jerzy Stojko, published by Research Institute of Horticulture
This work is licensed under the Creative Commons License.

Volume 56 (2012): Issue 1 (June 2012)