The assessment of doubled haploid lines obtained in pepper (Capsicum annuum L.) anther culture

Olszewska, Dorota; Kisiała, Anna; Nowaczyk, Paweł

doi:10.2478/v10245-011-0014-5

Abstract

The aim of the research was the analysis of 11 DH-R₂ pepper (Capsicum annuum L.) lines, obtained in anther cultures of ‘(ATZ1 × PO)’ F₁, ‘(ATZ1 × CDT)’ F₁ and ‘(ATZ1 × TG)’ F₁ hybrids. To determine the genetic homogeneity of anther-derived lines, the biometrical characteristics of fruits, as well as fruit colour and shape inheritance, were analysed. The biometrical analysis determined the highest phenotype uniformity in androgenic lines of ‘(ATZ1 × PO)’ F₁. Based on the fruit shape and colour variation, it was possible to determine the microspore origin of androgenic diploids obtained in anther cultures of ‘(ATZ1 × TG)’ F₁. Additionally, enzyme analysis of four isozymes (PGM, PGI, IDH and MDH) showed that isocitrate dehydrogenase identified anther-derived diploids of hybrids between ‘ATZ1’ and ‘TG’, as well as those between ‘ATZ1’ and ‘CDT’, lines.

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The assessment of doubled haploid lines obtained in pepper (Capsicum annuum L.) anther culture

Abstract

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