Occurrence and Expression of Toxin Genes in Clostridium Perfringens Isolates from Pigs

Kukier, Elżbieta; Goldsztejn, Magdalena; Grenda, Tomasz; Kwiatek, Krzysztof

doi:10.2478/v10213-012-0087-0

Abstract

Clostridium perfringens isolates were obtained from pigs of five porcine farms in Poland. The presence of C. perfringens was detected in 92% of faeces samples and its number ranged from 1.0 x 101 cfu/g to 1.2 x 107 cfu/g. All the isolates belonged to type A and 48.7% of them contained cpb2 gene. The qualitative assessment of toxin genes expression by type A subtype β2 isolates showed expression of cpa gene in 100% of strains and cpb2 gene in 71% of the analysed strains. The isolate from one-day-old piglets demonstrated also the expression of cpa and cpb2 genes.

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Occurrence and Expression of Toxin Genes in Clostridium Perfringens Isolates from Pigs

Abstract

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