Analysis of IL-2 Production in Hookworm Infected Hamsters Using Generated Polyclonal Antibodies

Długosz, Ewa; Cendrowski, Jarosław; Bąska, Piotr; Siwińska, Anna; Wędrychowicz, Halina; Wiśniewski, Marcin

doi:10.2478/v10213-012-0007-3

Abstract

The aim of the study was cloning and analysis of the entire coding sequence of hamster IL-2 by the method of RACE-PCR, its expression in Escherichia coli cells, and production of IL-2 specific antibodies. These antibodies were used to determine in vitro IL-2 production by cells derived from the spleen and mesenteric lymph nodes of Ancylostoma ceylanicum infected hamsters. The highest concentration of IL-2 was noted in supernatants from cell cultures coming from the oldest, most resistant hamsters.

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Analysis of IL-2 Production in Hookworm Infected Hamsters Using Generated Polyclonal Antibodies

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