Antigenotoxic effects of a bark extract from Magnolia officinalis

Zuzana Rybková; Kateřina Malachová

doi:10.2478/v10210-012-0028-9

Abstract

The aim was to detect antimutagenic and DNA protective effects of a plant extract from bark of Magnolia officinalis adversing oxidative DNA damage. The ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BOOH) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His- TA102 bacterial strain. Inhibition values of 72.8 and 98.7 % were detected for t-BOOH and H2O2, respectively. The protective effect of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in the plasmid DNA proved a significant inhibition of DNA damage.

References

Abràmof f M.D., Magalhães P.J. & Ram S.J. (2004): Image Processing with ImageJ. − Biophotonics Int. 11: 36-42.
Search in Google Scholar
Ames B.J., McCann J. & Yamasaki E. (1975): Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. − Mutat. Res. 31: 347-364.
Search in Google Scholar
Bang K.H., Kim Y.K., Min B.S., Na M.K., Rhee Y.H., Lee J.P. & Bae K.H. (2000): Antifungal Activity of Magnolol and Honokiol. − Arch. Pharm. Res. 23: 46-49.
Search in Google Scholar
Boubaker J., Skandrani I., Bouhlel I., Ben Sghaier M., Neffati A., Ghedira K. & Chekir - Ghedira L. (2010): Mutagenic, antimutagenic and antioxidant potency of leaf extracts from Nitrariaretusa. − Food Chem. Toxicol. 48: 2283-2290.
Search in Google Scholar
Broekhoven L.H & Nestmann E.R. (1991): Statistical analysis of the Salmonella mutagenicity assay, in: Krewski, D., Franklin, C. (Eds.), Statistics in Toxicology. − Gordon & Breach, Amsterdam: pp. 28-34.
Search in Google Scholar
Carino-Cortés R., Hernández-Ceruelos A., Torres -Valencia J.M., Gonzáles -Avi la M., Arriaga-Alba M. & Madrigal -Bujaidar E. (2007): Antimutagenicity of Stevia pilosa and Steviaeupatoria evaluated with the Ames test. − Toxicol. In Vitro 21: 691-697.10.1016/j.tiv.2006.12.001
Search in Google Scholar
Chang B., Lee Y., Ku Y., Bae K. & Chung C. (1998): Antimicrobial activity of magnolol and honokiol against periodontopathic microorganisms. − Planta Med. 64: 367-369.10.1055/s-2006-957453
Search in Google Scholar
Chen Y.H., Lin F.Y., Liu P.L., Huang Y.T., Chiu J.H., Chang Y.C., Man K.M., Hong C.Y., Ho Y.Y. & Lai M.T. (2009): Antioxidative and Hepatoprotective Effects of Magnolol on Acetaminopheninduced Liver Damage in Rats. − Arch. Pharm. Res. 32: 221-228.
Search in Google Scholar
Chiu J.H., Ho C.T., Wei Y.H., Lui W.Y. & Hong C.Y. (1997): In vitro and in vivo protective effect of honokiol on rat liver from peroxidative injury. − Life Sci. 61: 1961-1971.10.1016/S0024-3205(97)00836-9
Search in Google Scholar
Clark A.M., Elferaly F.S. & Li W.S. (1981): Antimicrobial activity of phenolic constituents of Magnolia grandiflora. − J. Pharm. Sci. 70: 951-952.
Search in Google Scholar
Fujita S. & Tai ra J. (1994): Biphenyl compounds are hydroxyl radical scavengers: their effective inhibition for UV-induced mutation in Salmonella typhimurium TA102. − Free Radical Biol. Med. 17: 273-277.
Search in Google Scholar
Haraguchi H., Ishikawa H., Shirataki N. & Fukuda A. (1997): Antiperoxidative activity of neolignans from Magnolia obovata. − J. Pharm. Pharmacol. 49: 209-212.
Search in Google Scholar
Ho K.Y., Tsai C.C., Chen C.P., Huang J.S. & Lin C.C. (2001): Antimicrobial Activity of Honokiol and Magnolol Isolated from Magnolia officinalis. − Phytother. Res. 15: 139-141.
Search in Google Scholar
Horn R.C. & Vargas V.M.F. (2008): Mutagenicity and antimutagenicity of teas used in popular medicine in the salmonella/microsome assay. − Toxicol. In Vitro 22: 1043-1049.10.1016/j.tiv.2007.12.014
Search in Google Scholar
Ikeda, K. & Nagase H. (2002): Magnolol Has the Ability to Induce Apoptosis in Tumor Cells. − Biol. Pharm. Bull. 25: 1546-1549.
Search in Google Scholar
Kris -Etherton P.M., Hecker K.D., Bonanome A., Coval S.M., Binkoski A.E., Hilper t K.F., Griel A.E. & Etherton T.D. (2002): Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. − Am. J. Med. 113: 71S-88S.10.1016/S0002-9343(01)00995-0
Search in Google Scholar
Li N., Song Y., Zhang W., Wang W., Chen J., Wong A.W. & Roberts A. (2007): Evaluation of the in vitro and in vivo genotoxicity of magnolia bark extract. − Regul. Toxicol. Pharmacol. 49: 154-159.
Search in Google Scholar
Lo Y.C., Teng C.M., Chen C.F., Chen C.C. & Hong C.Y. (1994): Magnolol and honokiol isolated from Magnolia officinalis protect rat heart mitochondria against lipid peroxidation. − Biochem. Pharmacol. 47: 549-553.
Search in Google Scholar
Makena P.S. & Chung K.T. (2007): Effects of various plant polyphenols on bladder carcinogen benzidine-induced mutagenicity. − Food Chem. Toxicol. 45: 1899-1909.
Search in Google Scholar
Manach C., Scalbert A., Morand C., Rémésy C. & J iménez L. (2004): Polyphenols: food sources and bioavailability. − Am. J. Clin. Nutr. 79: 727-747.
Search in Google Scholar
Margolin B.H., Kim B.S. & Risko K.J. (1989): The Ames Salmonella/Microsome Mutagenicity Assay: Issues of Inference and Validation. − J. Am. Stat. Assoc. 84: 651-661.
Search in Google Scholar
Maron D.M. & Ames B.N. (1983): Revised methods for the Salmonella mutagenicity test. − Mutat. Res. 113: 173-215.
Search in Google Scholar
Mortelmans K. & Zeiger E. (2000): The Ames Salmonella/microsome mutagenicity assay. − Mutat. Res. 455: 29-60.
Search in Google Scholar
Nagase H., Ikeda K. & Sakai Y. (2001): Inhibitory Effect of Magnolol and Honokiol from Magnoliaobovata on Human Fibrosarcoma HT-1080. Invasiveness in vitro. − Planta Med. 67: 705-708.10.1055/s-2001-1834511731909
Search in Google Scholar
Park E.J., Zhao Y.Z., Na M., Bae K., Kim Y.H., Lee B.H. & Sohn D.H. (2003): Protective Effects of Honokiol and Magnolol on Tertiary Butyl Hydroperoxide- or D-Galactosamine-Induced Toxicity in Rat Primary Hepatocytes. − Planta Med. 69: 33-37.10.1055/s-2003-3702712567276
Search in Google Scholar
Ramasamy K. & Agarwal R. (2008): Multitargeted therapy of cancer by silymarin. − Cancer Lett. 269: 352-362.10.1016/j.canlet.2008.03.053261299718472213
Search in Google Scholar
Saito J., Sakai Y. & Nagase H. (2006): In vitro anti-mutagenic effect of magnolol against direct and indirect mutagens. − Mutat. Res. 609: 68-73.
Search in Google Scholar
Scalbert A. & Wil liamson G. (2000): Dietary Intake and Bioavailability of Polyphenols. − J. Nutr. 130: 2073S-2085S.10.1093/jn/130.8.2073S10917926
Search in Google Scholar
Stagos D., Kazantzoglou G., Theofanidou D., Kakalopoulou G., Magiatis P., Mitaku S. & Kouretas D. (2006): Activity of grape extracts from Greek varieties of Vitis vinifera against mutagenicity induced by bleomycin and hydrogen peroxide in Salmonella typhimurium strain TA102. − Mutat. Res. 609: 165-175.10.1016/j.mrgentox.2006.06.03216935024
Search in Google Scholar
Taira J., Ikemoto T., Mimura K., Hagi A., Murakami A. & Makino K. (1993): Effective inhibition of hydroxyl radicals by hydroxylated biphenyl compounds. − Free Radic. Res. Commun. 19: 71-77.
Search in Google Scholar
Verhagen H., Rompelberg C.J.M., Strube M., Van Poppel G. & Van Bladeren P.J. (1997): Cancer prevention by dietary constituents in toxicological perspective. − J. Environ. Pathol. Toxicol. Oncol. 16: 343-360.
Search in Google Scholar
Wang J.P., Ho T.F., Chang L.C & Chen C.C. (1995): Anti-inflammatory effect of magnolol, isolated from Magnolia officinalis, on A23187-induced pleurisy in mice. − J. Pharm. Pharmacol. 47: 857-860.
Search in Google Scholar
Xia E.-Q., Deng G.-F., Guo Y.-J., Li H.-B. (2010): Biological activities of polyphenols from grapes. − Int. J. Mol. Sci. 11: 622-646.
Search in Google Scholar
Zhang Y.M. & Rock C.O. (2004): Evaluation of Epigallocatechin Gallate and Related Plant Polyphenols as Inhibitors of the FabG and FabI Reductases of Bacterial Type II Fatty-acid Synthase. − J. Biol. Chem. 279: 30994-31001.
Search in Google Scholar

Antigenotoxic effects of a bark extract from Magnolia officinalis

Abstract

Paradigm

My account