Abstract
In the present study, the effects coelomic fluid removal method (rinsing with clear water versus siphoning) and de-adhesion substances (milk solution, starch suspension and urea-NaCl-tannic acid solutions) had on fertilization rates and hatching success and time of Siberian sturgeon, Acipenser baerii Brandt were compared. It was concluded that 1) rinsing Siberian sturgeon eggs prior to fertilization is recommended when there is abundant, viscous coelomic fluid; 2) de-adhesion with milk or urea-NaCl-tannic acid is less time-consuming and results in higher, although not significantly, hatching percentages than those obtained with starch, and 3) de-adhesion with milk ensures the shortest and most synchronous hatching of Siberian sturgeon larvae. All of these issues require further experimental investigations.