Impact of iridovirus and methisoprinol on immunocompetent cells isolated from African catfish, Clarias gariepinus (Burchell), under in vitro conditions

Experiments to assess the effectiveness of the immunostimulator methisoprinol (Polfa Grodzisk Pharmaceuticals, Poland) focused on its impact on the innate immune response. The impact of different doses of methisoprinol on organ leukocytes isolated from the kidneys and spleens of African catfish that were subjected to or not subjected to the suppressive impact of iridovirus. The results indicate that the addition of methisoprinol causes increased respiratory burst activity (RBA), potential killing activity (PKA), and proliferative activity of lymphocytes T and B in response to mitogens. Methisoprinol stimulates the mechanisms of cellular immunity that are linked to the activation of T lymphocytes, which can impact the antiviral activity of this preparation after its application in vivo.