The Most Important Transcriptional Factors of Osteoblastogenesis

Witkowska-Zimny, Malgorzata; Wrobel, Edyta; Przybylski, Jacek

doi:10.2478/v10052-010-0002-x

Abstract

One of the key issues of organogenesis is the understanding of mechanisms underlying the differentiation of progenitor cells into more specialized cells of individual tissues. Recent transcriptomic and proteomic approaches of molecular biology have led to the identification of several factors and mechanisms regulating morphogenesis at the genetic level which affect the function of already differentiated cells. In the last few years, several reports about osteoblastogenesis have been published. This review presents recent findings on the role of the most important transcription factors supporting bone formation.

References

[1] AMORIM BR, OKAMURA H, YOSHIDA K, QIU L, MORIMOTO H, HANEJI T. The transcriptional factor Osterix directly interacts with RNA helicase A. Biochem Biophys Res Commun 2007; 355: 347-351.10.1016/j.bbrc.2007.01.15017303075
Search in Google Scholar
[2] BAE S.C., LEE YH. Phosphorylation, acetylation and ubiquitination: the molecular basis of RUNX regulation. Gene 2006; 366: 58-6610.1016/j.gene.2005.10.01716325352
Search in Google Scholar
[3] BAEK WY, LEE MA, JUNG JW, KIM SY, AKIYAMA H, DE CROMBRUGGHE B, KIM JE. Positive Regulation of Adult Bone Formation by Osteoblast-Specific Transcription Factor Osterix. J Bone Miner Res 2009; 24: 1055-106510.1359/jbmr.08124819113927402041619113927
Open DOI Search in Google Scholar
[4] BESSA PC, CASAL M, REIS RL. Bone morphogenetic proteins in tissue engineering: the road from laboratory to clinic, part II (BMP delivery). J Tissue Eng Regen Med 2008; 2: 81-96.1838345410.1002/term.7418383454
Open DOI Search in Google Scholar
[5] BESSA PC, CASAL M, REIS RL. Bone morphogenetic proteins in tissue engineering: the road from the laboratory to the clinic, part I (basic concepts). J Tissue Eng Regen Med 2008; 2: 1-13.10.1002/term.6318293427
Open DOI Search in Google Scholar
[6] BOBIS S, JAROCHA D, MAJKA M. Mesenchymal stem cells: characteristics and clinical applications. Folia Histochem Cytobiol 2006; 44: 215-230.17219716
Search in Google Scholar
[7] CADIGAN KM, LIU YI. Wnt signaling: complexity at the surface J Cell Science 2006; 119: 395-40210.1242/jcs.0282616443747
Search in Google Scholar
[8] CHOI SJ, SONG IS, RYU OH, CHOI SW, HART PS, WU WW, SHEN RF, HART TC. A 4 bp deletion mutation in DLX3 enhances osteoblastic differentiation and bone formation in vitro. Bone 2008; 42: 162-171.10.1016/j.bone.2007.08.047225367117950683
Open DOI Search in Google Scholar
[9] CHU S, FERRO TJ. Sp1: regulation of gene expression by phosphorylation. Gene 2005; 348: 1-11.10.1016/j.gene.2005.01.01315777659
Search in Google Scholar
[10] COOPER JA, JR., BAILEY LO, CARTER JN, CASTIGLIONI CE, KOFRON MD, KO FK, LAURENCIN CT. Evaluation of the anterior cruciate ligament, medial collateral ligament, achilles tendon and patellar tendon as cell sources for tissue-engineered ligament. Biomaterials 2006; 27: 2747-2754.10.1016/j.biomaterials.2005.12.0131641411516414115
Open DOI Search in Google Scholar
[11] DE FRUTOS CA, DACQUIN R, VEGA S, JURDIC P, MACHUCA-GAYET I, NIETO MA. Snail1 controls bone mass by regulating Runx2 and VDR expression during osteoblast differentiation. EMBO J 2009; 28: 686-996.10.1038/emboj.2009.23264777119197242
Open DOI Search in Google Scholar
[12] FLORES MV, TSANG VW, HU W, KALEV-ZYLINSKA M, POSTLETHWAIT J, CROSIER P, CROSIER K, FISHER S. Duplicate zebrafish runx2 orthologues are expressed in developing skeletal elements. Gene Expr Patterns 2004; 4: 573-581.10.1016/j.modgep.2004.01.01615261836
Open DOI Search in Google Scholar
[13] GIULIANI N, COLLA S, MORANDI F, LAZZARETTI M, SALA R, BONOMINI S, GRANO M, COLUCCI S, SVALDI M, RIZZOLI V. Myeloma cells block RUNX2/CBFA1 activity in human bone marrow osteoblast progenitors and inhibit osteoblast formation and differentiation. Blood 2005; 106: 2472-2483.10.1182/blood-2004-12-498615933061
Search in Google Scholar
[14] HANDSCHIN AE, EGERMANN M, WEDLER V, TRENTZ O, HEMMI S, TRENTZ OA. A comparative analysis of phenotype expression in human osteoblasts from heterotopic ossification and normal bone. Langenbecks Arch Surg 2006; 391: 376-38210.1007/s00423-005-0021-516501989
Search in Google Scholar
[15] HASSAN MQ, JAVED A, MORASSOM MI, KARLIN J, MONTECINO M, van WIJNEN AJ, STEIN GS, STEIN JL, LIAN JB. Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene. Mol Cell Biol 2004; 24 :9248-926110.1128/MCB.24.20.9248-9261.200451787315456894
Open DOI Search in Google Scholar
[16] HASSAN MQ, TARE RS, LEE SH, MANDEVILLE M, MORASSO MI, JAVED A, VAN WIJNEN AJ, STEIN JL, STEIN GS, LIAN JB. BMP2 commitment to the osteogenic lineage involves activation of Runx2 by DLX3 and a homeodomain transcriptional network. J Biol Chem 2006; 281: 40515-40526.10.1074/jbc.M60450820017060321
Search in Google Scholar
[17] HASSAN MQ, TARE R, LEE SH, MANDEVILLE M, WEINER B, MONTECINO M, VAN WIJNEN AJ, STEIN JL, STEIN GS, LIAN JB. HOXA10 controls osteoblastogenesis by directly activating bone regulatory and phenotypic genes. Mol Cell Biol 2007; 27: 3337-3352.10.1128/MCB.01544-06189996617325044
Open DOI Search in Google Scholar
[18] HASSAN MQ, SAINI S, GORDON JA, VAN WIJNEN AJ, MONTECINO M, STEIN JL, STEIN GS, LIAN JB. Molecular switches involving homeodomain proteins, HOXA10 and RUNX2 regulate osteoblastogenesis. Cells Tissues Organs 2009; 189: 122-125.10.1159/000151453266570518701816
Search in Google Scholar
[19] HATTA M, YOSHIMURA Y, DEYAMA Y, FUKAMIZU A, SUZUKI K. Molecular characterization of the zinc finger transcription factor, Osterix. Int J Mol Med 2006; 17: 425-430.1646538810.3892/ijmm.17.3.425
Search in Google Scholar
[20] ISA ZM, SCHNEIDER GB, ZAHARIAS R, SEABOLD D, STANFORD CM. Effects of fluoridemodified titanium surfaces on osteoblast proliferation and gene expression. Int J Oral Maxillofac Implants 2006; 21: 203-211.16634490
Search in Google Scholar
[21] JEONG JH, JIN JS, KIM HN, KANG SM, LIU JC, LENGNER CJ, OTTO F, MUNDLOS S, STEIN JL, VAN WIJNEN AJ, LIAN JB, STEIN GS, CHOI JY. Expression of Runx2 transcription factor in non-skeletal tissues, sperm and brain. J Cell Physiol 2008; 217: 511-517.10.1002/jcp.21524261258818636555
Search in Google Scholar
[22] KASSEM M, ABDALLAH BM, SAEED H. Osteoblastic cells: Differentiation and transdifferentiation. Arch Biochem Biophys 2008; 473: 183-18710.1016/j.abb.2008.03.02818406334
Search in Google Scholar
[23] KOGA T, MATSUI Y, ASAGIRI M, KODAMA T, DE CROMBRUGGHE B, NAKASHIMA K, TAKAYANAGI H. NFAT and Osterix cooperatively regulate bone formation. Nat Med 2005; 11: 880-885. 1604138410.1038/nm1270
Open DOI Search in Google Scholar
[24] KOMORI T. Mechanism of transcriptional regulation by Runx2 in osteoblasts. Clin Calcium 2006; 16: 801-807.
Search in Google Scholar
[25] LEONG WF, ZHOU T, LIM GL, LI B. Protein palmitoylation regulates osteoblast differentiation through BMP-induced osterix expression. PLoS ONE 2009; 4: e4135.10.1371/journal.pone.0004135
Open DOI Search in Google Scholar
[26] LI Y , XIAO Z. Advances in Runx2 regulation and its isoforms. Med Hypotheses 2007, 68: 169-17510.1016/j.mehy.2006.06.006
Search in Google Scholar
[27] LIEDERT A, KASPAR D, CLAES L, IGNATIUS A. Signal transduction pathways involved in mechanical regulation of HB-GAM expression in osteoblastic cells. Biochem Biophys Res Commun 2006; 342: 1070-1076.10.1016/j.bbrc.2006.02.063
Search in Google Scholar
[28] LIU W, TOYOSAWA S, FURUICHI T, KANATANI N, YOSHIDA C, LIU Y, HIMENO M, NARAI S, YAMAGUCHI A, KOMORI T. Overexpression of Cbfa1 in osteoblasts inhibits osteoblast maturation and causes osteopenia with multiple fractures. J Cell Bio. 2001 ;155: 157-166.10.1083/jcb.200105052
Search in Google Scholar
[29] MAKITA N, SUZUKI M, ASAMI S, TAKAHATA R, KOHZAKI D, KOBAYASHI S, HAKAMAZUKA T, HOZUMI N. Two of four alternatively spliced isoforms of RUNX2 control osteocalcin gene expression in human osteoblast cells. Gene 2008; 413: 8-17.10.1016/j.gene.2007.12.025
Search in Google Scholar
[30] MARIE PJ. Transcription factors controlling osteoblastogenesis. Arch Biochem Biophys 2008; 473: 98-105.10.1016/j.abb.2008.02.030
Search in Google Scholar
[31] MASAKI C, SCHNEIDER GB, ZAHARIAS R, SEABOLD D, STANFORD C. Effects of implant surface microtopography on osteoblast gene expression. Clin Oral Implants Res 2005; 16: 650-656.10.1111/j.1600-0501.2005.01170.x16307571
Open DOI Search in Google Scholar
[32] MBALAVIELE G, SHEIKH S, STAIN JP, SALAZAR VS, CHENG SL, CHEN D, CIVITELLI R. Beta-catenin and BMP-2 synergize to promote osteoblast differentiation and new bone formation. J Cell Biochem 2005 ;94: 403-418.10.1002/jcb.2025315526274
Open DOI Search in Google Scholar
[33] NAKASHIMA K, ZHOU X, KUNKEL G, ZHANG Z, DENG JM, BEHRINGER RR, DE CROMBRUGGHE B. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 2002; 108: 17-29.10.1016/S0092-8674(01)00622-511792318
Open DOI Search in Google Scholar
[34] NEMOTO Y, HIGUCHI K, BABA O, KUDO A, TAKANO Y. Multinucleate osteoclasts in medaka as evidence of active bone remodeling. Bone 2007; 40: 399-408.1704932710.1016/j.bone.2006.08.019
Open DOI Search in Google Scholar
[35] NOHE A, KEATING E, KNAUS P, PETERSEN NO. Signal transduction of bone morphogenetic protein receptors. Cell Signal 2004; 16: 291-299.1468765910.1016/j.cellsig.2003.08.011
Open DOI Search in Google Scholar
[36] OKAMURA H, AMORIM BR, WANG J, YOSHIDA K, HANEJI T. Calcineurin regulates phosphorylation status of transcription factor osterix. Biochem Biophys Res Commun 2009; 379: 440-444.10.1016/j.bbrc.2008.12.094
Search in Google Scholar
[37] OSYCZKA AM, LEBOY PS. Bone morphogenetic protein regulation of early osteoblast genes in human marrow stromal cells is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3-kinase signaling. Endocrinology 2005; 146: 3428-3437.10.1210/en.2005-0303
Search in Google Scholar
[38] OTTO F, THORNEL AP, CROMPTON T, DENZEL A, GILMOUR KC, ROSEWELL IR, STAMP GWH, BEDDINGTON RSP, MUNDLOS S, OLSEN BR, SELBY PB, OWEN MJ. CbfaI, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 1997; 89: 765-77110.1016/S0092-8674(00)80259-7
Search in Google Scholar
[39] PAZ J, WADE K, KIYOSHIMA T, SODEK J, TANG J, TU Q, YAMAUCHI M, CHEN J. Tissueand bone cell-specific expression of bone sialoprotein is directed by a 9.0 kb promoter in transgenic mice. Matrix Biol 2005; 24: 341-352.10.1016/j.matbio.2005.05.00915970437
Open DOI Search in Google Scholar
[40] PHILIPSEN S, SUSKE G. A tale of three fingers: the family of mammalian Sp/XKLF transcription factors. Nucleic Acids Res 1999; 27: 2991-3000.1045459210.1093/nar/27.15.299114852210454592
Open DOI Search in Google Scholar
[41] PHIMPHILAI M, ZHAO Z, BOULES H, ROCA H, FRANCESCHI RT. BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype. J Bone Miner Res 2006; 21: 637-646.10.1359/jbmr.060109243517116598384
Open DOI Search in Google Scholar
[42] RYO HM, LEE MH, KIM YJ. Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells. Gene 2006, 366: 51-5710.1016/j.gene.2005.10.01116314053
Search in Google Scholar
[43] SAMEE N, GEOFFROY V, MARTY C, SCHILTZ C, VIEUX-ROCHAS M, LEVI G, DE VERNEJOUL MC. Dlx5, a positive regulator of osteoblastogenesis, is essential for osteoblastosteoclast coupling. Am J Pathol 2008; 173: 773-780.10.2353/ajpath.2008.080243252708918669617
Search in Google Scholar
[44] WESTENDORF JJ, KAHLER RA, SCHROEDER TM. Wnt signaling in osteoblasts and bone diseases. Gene 2004; 341: 19-39.10.1016/j.gene.2004.06.04415474285
Search in Google Scholar
[45] WIREN KM, TOOMBS AR, ZHANG XW. Androgen inhibition of MAP kinase pathway and Elk-1 activation in proliferating osteoblasts. J Mol Endocrinol 2004; 32: 209-226. 10.1677/jme.0.03202091476600314766003
Open DOI Search in Google Scholar
[46] ZAKANY J, DUBOULE D. The role of Hox genes during vertebrate limb development. Curr Opin Genet Dev 2007 ;17: 359-366. 10.1016/j.gde.2007.05.01117644373
Open DOI Search in Google Scholar

The Most Important Transcriptional Factors of Osteoblastogenesis

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