Role of Carboxyl Groups in the Secondary Structure and Function of Sturgeon Gonadotropin

Henriks Zenkevičs; Ilze Vosekalna; Vija Vose

doi:10.2478/v10046-008-0014-1

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Role of Carboxyl Groups in the Secondary Structure and Function of Sturgeon Gonadotropin

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

Volume 62 (2008): Issue 3 (June 2008)

By: Henriks Zenkevičs, Ilze Vosekalna and Vija Vose

Open Access

|Nov 2008

Abstract

Free negatively charged carboxyl groups were selectively modified (neutralised) in sturgeon (Acipenser güldenstädti Br.) gonadotropic hormone (GTH) α and β subunits. Eleven free carboxyl groups, three in the α and eight in the β subunit, were neutralised by reaction with glycine ethyl ester. Investigation of reassociated α-β dimers (recombinants) comprising one or both modified subunits showed that specific hormonal activity was completely lost while immunoreactivity was lowered in comparison with that of the standard α-β dimer. CD-spectroscopy of the modified subunits did not indicate any considerable changes in their spatial structure. A conclusion was made that free COOH groups of GTH were important as bearers of the negative charge necessary for the hormone activity on the level of the hormone-specific membrane receptors.

References

Faith, M. R., Pierce, J. G. (1975). The carboxylic acid groups of bovine luteinizing hormone. (The effect of their modification on receptor site binding and subunit-subunit interaction). J. Biol. Chem., 250(17), 6923-6929.
Search in Google Scholar
Hennen, G., Prusik, Z., Maghuin-Rogister, G. (1971). Porcine luteinizing hormone and its subunits. Eur. J. Biochem., 18, 376-383.10.1111/j.1432-1033.1971.tb01253.x
Search in Google Scholar
Lees, J. G., Miles, A. J., Wien, F., Wallace, B. A. (2006). A reference database for circular dichroism spectroscopy covering fold and secondary structure space. Bioinformatics, 22(16), 1955-1962.10.1093/bioinformatics/btl327
Search in Google Scholar
Ouchterlony, O. (1958). Diffusion-in-gel methods for immunological analyses. Progr. Allergy, 5, 1-6.
Search in Google Scholar
Sreerama, N., Venyaminov, S. Y., Woody, R. W. (1999). Estimation of the number of alpha- helical and beta-strand segments in proteins using circular dichroism spectroscopy. Protein Sci., 8, 370-380.10.1110/ps.8.2.370
Search in Google Scholar
Thornton, V. F. (1971). A bioassay for progesterone and gonadotropins based on meiotic division of Xenopus oocytes in vitro. Gen. Comp. Endocrinol., 16, 599-605.10.1016/0016-6480(71)90125-0
Search in Google Scholar
Whitmore, L., Wallace, B. A. (2004). DICHROWEB, an online server of protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acid Res., 32, W668-W673.10.1093/nar/gkh37144150915215473
Search in Google Scholar
Zenkevičs, H. (1994). Fish gonadotropic hormone: Structural-functional investigations. Proc. Latvian Acad. Sci., Section B, 48(5/6), 82-88.
Search in Google Scholar
Zenkevičs, H., Vose, V., Būcena, A. (1999). Substitution of native subunits in sturgeon gonadotropin. Proc. Latvian Acad. Sci., Section B, 53(5), 261-264.
Search in Google Scholar
Zenkevičs, H., Vose, V., Būcena, A. (2003). Functional role of tryptophan residues in sturgeon gonadotropic hormone. Proc. Latvian Acad. Sci., Section B, 57(5), 187-190.
Search in Google Scholar
Зенкевич Г. А. Гона∂омроньιŭ ςормон рьιб: нолученuе u своŭсмва [Fish Gonadotropic Hormone: Isolation and Properties]. Рига, зинатне. 224 c. (in Russian).
Search in Google Scholar
Зенкевич Г. А. (1992). Арбатский Н. П., Сланке Б. П., Желтова А. О., ервицкая В. А. (1992). Стыктура угледньіх цепей димерной молекульі и отдельньіх субъединиц гонадотропина русского осетра [Structure of the carbohydrate chains of the dimeric molecule and individual subunits of the Russian sturgeon gonadotropin]. Бuоорςанuческаая хuмuя 18, 226-234 (in Russian).
Search in Google Scholar
Зенкевич Г. А. Сланке Б. П., Лаце З. М. Кирстукас И. П. (1987). Свойства субъединиц как компонетов антигенной структурьі гонадотрпина осетра [Properties of sturgeon gonadotropin subunits as the components of the hormone antigenic structure]. Доклаьі Акабемuu Наук CCCP, 297, 1480-1482 (in Russian).
Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.2478/v10046-008-0014-1 | Journal eISSN: 2255-890X | Journal ISSN: 1407-009X

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Language: English

Page range: 110 - 114

Published on: Nov 29, 2008

Published by: Latvian Academy of Sciences

In partnership with: Paradigm Publishing Services

Publication frequency: 6 issues per year

Keywords:

fish gonadotropic hormone,

subunits,

chemical modification,

Related subjects:

© 2008 Henriks Zenkevičs, Ilze Vosekalna, Vija Vose, published by Latvian Academy of Sciences
This work is licensed under the Creative Commons License.

Volume 62 (2008): Issue 3 (June 2008)