The Microtox® biological test: Application in toxicity evaluation of surface waters and sediments in Poland

Niemirycz, Elżbieta; Nichthauser, Joanna; Staniszewska, Marta; Nałęcz-Jawecki, Grzegorz; Bolałek, Jerzy

doi:10.2478/v10009-007-0030-5

Abstract

Research into the suitability of Microtox® for the evaluation of toxicity of surface waters and bottom sediments in Poland was conducted. Water bodies of various pollution levels were tested, including the Odra River and its tributaries, the Lower Vistula River, the Kashubian Lake District and the Gulf of Gdańsk, using a Microtox® Model 500 analyser (Microbics Corporation, USA). The majority of tested surface water samples were found to be apparently non-toxic. However, 75% of the bottom sediment samples were found to be highly toxic (EC50>2%). These results indicate that the Microtox® test is suitable for evaluating the toxicity of bottom sediments, in which pollutants tend to accumulate. It seems, however, that Microtox® lacks the sensitivity to be of use in analyzing water quality. The relationship between the toxicity of analysed sediments and their organic content was examined.

References

Blaise, C., Ferard, J.F. (2005). Effluent assessment with the PEEP (potential ecotoxic effects probe) index. In: Blaise, C. and Ferard, J.F. (Eds.), Small-scale freshwater toxicity inyestigations (Vol. 2, pp. 69-87). Netherlands: Springer. DOI: 10.1007/1-4020-3553-5-1.
Search in Google Scholar
Boluda, R., Quintanilla, J.F., Bonilla, J.A., Saez, E., Gamoń, M. (2002). Application of the Microtox® test and pollution indices to the study of water toxicity in the Albufera Natural Park (Valencia, Spain). Chemosphere, 46(2), 355-369. DOI:10.1016/S0045-6535(01)00092-3.10.1016/S0045-6535(01)00092-3
Search in Google Scholar
Cairns, J.J., Pratt, J.R. (1991). The scientific basic bioassays. Hydrobiologia, 188/189, 5-12.
Search in Google Scholar
Dmitruk, U., Wojciechowska, J. (1996). An evaluation of toxicity method using the bioluminescence. (Polish) Subject KBN W-1: New analytical methods for assesment of chemical and biological water and wastewater quality. Warsaw: The statutory activity of Institute of Meteorology and Water Management.
Search in Google Scholar
Fernández, A., Tejedor, C., Cabrera, F., Chordi, A. (1995). Assesment of toxicity of river water and effluents by the bioluminescence assay using Photobacterium phosphoreum. Water Res., 29(5), 1281-1286. DOI: 10.1016/0043-1354(94)00293-G.10.1016/0043-1354(94)00293-G
Search in Google Scholar
Grant, A. Briggs, A.D. (2002). Toxicity of sediments from around a North Sea oil platform: are metals or hydrocarbons responsible for ecological impacts? Marine Environ. Res., 53(1), 95-116. DOI:10.1016/S0141-1136(01)00114-3.10.1016/S0141-1136(01)00114-3
Search in Google Scholar
Greene, S.R., Bulich, A.A., Underwood, S.R. (1992). Measurement of soil and sediment toxicity to bioluminescent bacteria when in direct contact for a fixed time period. In: Proceedings of the Water Environ. Fed. 65^th Annual Conference and Exposition, 20-24 September 1992 (pp. 53-53). New Orleans, Louisiana, USA.
Search in Google Scholar
Gutierrez, M., Etxebarria, J., de las Fuentes, L. (2002). Evaluation of wastewater toxicity: comparative study between Microtox® and activated sludge oxygen uptake inhibition. Water. Res., 36(4), 919-924. DOI:10.1016/S0043-1354(01)00299-8.10.1016/S0043-1354(01)00299-8
Search in Google Scholar
ISO 11348 : 1998. (1998). Water quality. Determination of the inhibitory effects of water samples on the light emission of Vibrio fischeri.
Search in Google Scholar
Kemble, N.E., Hardesty, D.G., Ingersoll, C.G., Johnson, B.T., Dwyer, F.J., MacDonald, D.D. (2000). An evaluation of the toxicity of contaminated sediments from Waukegan Harbor, Illinois, following remediation. Arch. Environ. Contam. Toxicol., 39(4), 452-461. DOI: 10.1007/s002440010127.10.1007/s002440010127
Search in Google Scholar
Krebs, F. (2005). The pT-method as a hazard assessment scheme for wastewaters. In: Blaise, C. and Ferard. J.F. (Eds.), Small-scale freshwater toxicity investigations, (Vol., pp. 2115-137), Netherlands: Springer.
Search in Google Scholar
Kwan, K.K. and Dutka, B.J. (1992). Evaluation of Toxi-Chromotest Direct Sediment Toxicity. Testing Procedure and Microtox Solid-Phase. Testing Procedure. Bull. Environ. Contam. Toxicol., 49(5), 656-662. DOI: 10.1007/BF00200777.10.1007/BF00200777
Search in Google Scholar
Meyer, A. K. (Eds.) (2002). Die Belastung der Oder, Eisse des Internationalen Oderprojekts (IOP). Germany: Universität Hamburg, Print und Mail.
Search in Google Scholar
Michniewicz, M., Nałęcz-Jawecki, G., Stufka-Olczyk, J., Sawicki, J. (2000). Comparison of chemical composition and toxicity of wastewaters from pulp industry. In: Persoone, G., Janssen, C. and De Coen, W.M. (Eds.). New microbiotests for routine toxicity screening and biomonitoring (pp. 401-411). New York: Kluwer Academic/Plenum Publishers.
Search in Google Scholar
Microbics Corp. Microtox® Acute Toxicity Solid-Phase Test (1995) Microbics Corp., Carlsbad, CA.
Search in Google Scholar
Nałęcz-Jawecki, G., Sawicki, J. (1996). Microtox - quick test for assessment of toxicity of waters and wastewaters samples. (Polish) Gaz, Woda i Technika Sanitarna, 2, 47-51.
Search in Google Scholar
Niemirycz, E. (2006). Halogenated organic compounds in the main polish rivers and transport these compounds into the Baltic Sea. (Polish) Unpublished doctoral dissertation, Monographers of Institute of Meteorology and Water Management, Warsaw.
Search in Google Scholar
Niemirycz, E., Bierawska, B., Błażejowski, J., Chodyńska, A., Chyrek, B., Dojlido, J., Heybowicz, E., Judycka, U., Kaczmarczyk, A., Lammek, B., Namsolleck, P., Taylor, R., Sosnowska, M., Szczerski, R., Szafranek, J. (1998). Anthropogenic Organic Substances, Origin, Distribution and Impact on the Water Ecosystem. The International Odra Project, Annual Report 1998, Interim Report, Germany: Hamburg University.
Search in Google Scholar
Niemirycz, E., Bierawska, B., Błażejowski, J., Chyrek, B., Dojlido, J., Heybowicz, E., Judycka, U., Kaczmarczyk, A., Lammek, B., Makowski, Z., Sosnowska, M., Szafranek, J., Szczerski, R., Taylor, R. (1999). Anthropogenic Organic Substances, Origin, Distribution and Impact on the Water Ecosystem. The International Odra Project, Annual Report 1999, Interim Report, Germany: Hamburg University.
Search in Google Scholar
Niemirycz, E., Kaczmarczyk, A., Rodziewicz, M., Sapota, G., Sapota, M., Heybowicz, E., Bogacka, T., Taylor, R. (2002). Assessment of quality of the Odra and the Vistula mouth cross sectionsin relation to persistent organic pollutants included in the Stockholm Convention. (Polish) Report GF-POL-INV-R3.PDF of the project GF/POL/01/004, UNIDO, Gdańsk http://ios.edu.pl
Search in Google Scholar
Płaza, G. (2000). Bioluminescence as the toxicity index. (Polish), Gospodarka Wodna, 2, 6-9.
Search in Google Scholar
PN-75 C-04616/01 Water and wastewaters. Determination of water, dry mass, organic and mineral compounds in sludge sediments. (Polish)
Search in Google Scholar
Rogers, K.R., Harper, S.L., Robertson, G. (2005). Screening for toxic industrial chemicals using semipermeable membrane devices with rapid toxicity assays. Anal. Chim. Acta, 543(1-2), 229-235. DOI: 10.1016/j.aca.2005.04.016.10.1016/j.aca.2005.04.016
Search in Google Scholar
Tay, K., Doe, K.G., Wade, S.J., Vaughan, D.A., Berrigan, R.E. and Moore, M.J. (1992). Sediment bioassessment in Halifax Harbour. Environ. Toxicol Chem., 11(11), 1567-1581. DOI: 10.1897/1552-8618(1992)11[1567:SBIHH]2.0.CO;2
Search in Google Scholar
Tung, K.K., Scheibner, M.G., Walbourn, C.C. (1990). The solid phase assay: New Microtox test procedure. Proceedings of 17^th Annual Aquatic Toxicity Workshop, 5-7 November 1990, Vancouver, B.C. Vol. 1
Search in Google Scholar
Vasseur, P., Ferard, J.F., Rast, C., Weingertner, P. (1986). Le test Microtox et le controle de la qualite des eaux. d'Hydrol.17, 153-162.10.1051/water/19861702153
Search in Google Scholar
Vigano, L., Arillo, A., Buffagni, A., Camusso, M., Ciannarella, R., Crosa, G., Falugi, C., Galassi, S., Guzzella, L., Lopez, A., Mingazzini, M., Pagnotta, R., Patrolecco, L., Tartari, G., Valsecchi, S. (2003). Quality assessment of bed sediments of the Po River (Italy). Water Res., 37(3), 501-518. DOI:10.1016/S0043-1354(02)00109-4.10.1016/S0043-1354(02)00109-4
Search in Google Scholar
Voivodeship Inspectorate for Environmental Protection in Gdańsk (2000). Report of environmental quality in the Pomeranian Voivodeship at 1999. (Polish). Gdańsk: Library of State Environmental Monitoring.
Search in Google Scholar
Żelechowska, A, Makowski, Z, Michalska, M. (1996). Application of Microtox test for determination of acute toxicity of wastewaters and water. (Polish) KBN W-1 New analytical methods for chemical and physical evaluation of water and sludge quality. Gdańsk: The statutory activity of Institute of Meteorology and Water Management.
Search in Google Scholar

The Microtox® biological test: Application in toxicity evaluation of surface waters and sediments in Poland

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