Antitumour evaluation of di-(2-ethylhexyl) phthalate (DEHP) isolated from Calotropis gigantea L. flower / Evaluacija antitumorskog djelovanja di-(2-etilheksil)-ftalata (DEHP) izoliranog iz cvjetova Calotropis gigantea L.

Habib, Muhammad Rowshanul; Karim, Muhammad Rezaul

doi:10.2478/v10007-012-0035-9

Abstract

The objective of the study is to explore the anticancer activity of di-(2-ethylhexyl) phthalate (DEHP) isolated from Calotropis gigantea flower against Ehrlich ascites carcinoma cells (EAC) in Swiss albino mice. The activity of DEHP was evaluated at doses of 10, 20 and 40 mg kg^-1 body mass applied intraperitoneally. DEHP showed a significant decrease in viable cell count (p < 0.05), mass gain (due to tumour burden) and elevated the life span of EAC cell bearing mice. Altered hematological profiles such as RBC, hemoglobin, WBC and differential count were reverted to normal levels in DEHP-treated mice. DEHP also brought back altered biochemical parameters (glucose, cholesterol, triglycerides, blood urea, SALP and SGOT) to normal level. Results of this study indicate that DEHP show potent dose dependent antitumour activity against EAC in vivo.

References

1. D. J. Newman, G. M. Cragg and K. M. Snader, Natural products as sources of new drugs over the period 1981-2002, J. Nat. Prod. 66 (2003) 1022-1037; DOI: 10.1021/np030096l.10.1021/np030096l
Search in Google Scholar
2. Z. N. Wang, M. Y. Wang, W. L. Mei, Z. Han and H. F. Dai, A new cytotoxic pregnanone from Calotropis gigantea, Molecules 13 (2008) 3033-3039; DOI: 10.3390/molecules13123033.10.3390/molecules13123033
Search in Google Scholar
3. A. K. Pathak and A. Argal, CNS activity of Calotropis gigantea roots, J. Ethnopharmacol. 106 (2006) 142-145; DOI: 10.1016/j.jep.2005.12.024.10.1016/j.jep.2005.12.024
Search in Google Scholar
4. A. K. K. Mueen, A. C. Rana and V. K. Kixit, Calotropis species (Asclepiadaceae): A comprehensive review, Pharmacog. Mag. 1 (2005) 48-52.
Search in Google Scholar
5. M. R. Habib, A. A. Aziz and M. R. Karim, Inhibition of Ehrlich’s ascites carcinoma by ethyl acetate extract from the flower of Calotropis gigantea L. in mice, J. Appl. Biomed. 8 (2010) 47-54; DOI 10.2478/v10136-009-0007-7.10.2478/v10136-009-0007-7
Search in Google Scholar
6. M. Jesmin, M. M. Ali and J. A. Khanam, Antitumour activities of some schiff bases derived from benzoin, salicylaldehyde, amino phenol and 2,4 dinitrophenyl hydrazine, Thai J. Pharm. Sci. 34 (2010) 20-31.
Search in Google Scholar
7. P. Amade, M. Mallea and N. Bouaicha, Isolation, structure identification and biological activity of two metabolites produced by Penicillium olsonii Bainier and Sartory, J. Antibiot. 47 (1994) 201-207.10.7164/antibiotics.47.201
Search in Google Scholar
8. G. N. Rao, P. M. Kumar, V. S. Dhandapani, T. R. Krishna and T. Hayashi, Constituents of Cassia auriculata, Fitoterapia 71 (2000) 82-83; DOI: 10.1016/S0367-326X(99)00108-2.10.1016/S0367-326X(99)00108-2
Search in Google Scholar
9. H. Mavar-Manga, M. Haddad, L. Pieters, C. Baccelli, A. Penge and J. Quetin-Leclercq, Anti-inflammatory compounds from leaves and root bark of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg., J. Ethnopharmacol. 115 (2008) 25-29; DOI: 10.1016/j.jep.2007.08.043.10.1016/j.jep.2007.08.04317942256
Search in Google Scholar
10. K. H. Lee, J. H. Kim, D. S. Lim and C. H. Kim, Anti-leukaemic and anti-mutagenic effects of di-(2-ethylhexyl) phthalate isolated from Aloe vera Linn., J. Pharm. Pharmacol. 52 (2000) 593-598; DOI: 10.1211/0022357001774246.10.1211/002235700177424610864149
Search in Google Scholar
11. R. M. David, M. R. Moore, D. C. Finney and D. Guest, Chronic toxicity of di-(2-ethylhexyl) phthalate in mice, Toxicol. Sci. 58 (2000) 377-385; DOI: 10.1093/toxsci/58.2.377.10.1093/toxsci/58.2.37711099649
Search in Google Scholar
12. A. Bala, B. Kar, P. K. Haldar, U. K. Mazumder and S. Bera, Evaluation of anticancer activity of Cleome gynandra on Ehrlich’s ascites carcinoma treated mice, J. Ethnopharmacol. 129 (2010) 131-134; DOI: 10.1016/j.jep.2010.03.010.10.1016/j.jep.2010.03.01020307641
Search in Google Scholar
13. M. Gupta, U. K. Mazumder, R. S. Kumar, T. Sivakumar and M. L. M. Vamsi, Antitumor activity and antioxidant status of Caesalpinia bonducella against Ehrlich ascites carcinoma in Swiss albino mice, J. Pharmacol. Sci. 94 (2004) 177-184, DOI: 10.1254/iphs.94.177.
Search in Google Scholar
14. R. A. Killington, A. E. Williams, N. A. Ratchffe, T. P. Whitehead and H. Smith, hypoglycaemia and malignancy differences of closely related sublines of a rat tumour, Brit. J. Cancer. 25 (1971) 93-102; DOI: 10.1038/bjc.1971.13.10.1038/bjc.1971.1320085555581305
Search in Google Scholar
15. P. Saha, U. K. Mazumder, P. K. Halder, S. Naskar, S. Kundu, A. Bala and B. Kar, Anticancer activity of methanol extract of Cucurbita maxima against Ehrlich ascites carcinoma, Int. J. Res. Pharm. Sci. 2 (2011) 52-59.
Search in Google Scholar

Antitumour evaluation of di-(2-ethylhexyl) phthalate (DEHP) isolated from Calotropis gigantea L. flower / Evaluacija antitumorskog djelovanja di-(2-etilheksil)-ftalata (DEHP) izoliranog iz cvjetova Calotropis gigantea L.

Abstract

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