Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

Zohreh Mohammadi; Farid Dorkoosh; Saman Hosseinkhani; Tina Amini; Amir Rahimi; Abdolhossein Najafabadi; Morteza Tehrani

doi:10.2478/v10007-012-0008-z

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Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

Acta Pharmaceutica

Volume 62 (2012): Issue 1 (March 2012)

By: Zohreh Mohammadi, Farid Dorkoosh, Saman Hosseinkhani, Tina Amini, Amir Rahimi, Abdolhossein Najafabadi and Morteza Tehrani

Open Access

|Apr 2012

Abstract

A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing biotechnological products such as plasmids and targeting peptides. Chitosan-DNA-FAP-B nanoparticles are novel non-viral vectors for specific gene delivery to the lung epithelial cells. In this study, the storage stability of chitosan-DNA-FAP-B nanoparticles at -20, 5 and 24 °C was examined. Size, zeta potential and transfection efficiency of these nano-particles in storage were also evaluated. Stability studies showed that chitosan-DNA-FAP-B nanoparticles were stable after 1 month when stored at -20 °C and retained their initial size, zeta potential and transfection efficiency. However, their stability was not desirable at 5 and 24 °C. Based on these results, it can be concluded that chitosan-DNA-FAP-B nanoparticles can be a promising candidate for gene delivery to lung epithelial cells with good storage stability at -20 °C during 1 month.

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Articles in this issue

DOI: https://doi.org/10.2478/v10007-012-0008-z | Journal eISSN: 1846-9558 | Journal ISSN: 1330-0075

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Language: English, Croatian, Slovenian

Page range: 83 - 92

Published on: Apr 3, 2012

Published by: Croatian Pharmaceutical Society

In partnership with: Paradigm Publishing Services

Keywords:

FAP-B,

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© 2012 Zohreh Mohammadi, Farid Dorkoosh, Saman Hosseinkhani, Tina Amini, Amir Rahimi, Abdolhossein Najafabadi, Morteza Tehrani, published by Croatian Pharmaceutical Society
This work is licensed under the Creative Commons License.

Volume 62 (2012): Issue 1 (March 2012)