Assay of lercanidipine hydrochloride in dosage forms using nucleophilic substitution reaction

Sastry, Thuttagunta; Ramakrishna, Karipeddi

doi:10.2478/v10007-011-0031-5

Abstract

A simple and sensitive spectrophotometric method has been developed for the assay of lercanidipine hydrochloride (LER) in bulk and in formulations. The method is based on the formation of coloured species between the drug and 1,2-naphthaquinone-4-sulphonic acid sodium salt (NQS) by means of nucleophilic substitution reaction. Absorbance was measured at λ_max = 460 nm. The method was analyzed statistically. The system obeyed the Beer's law in the range 20-100 μg mL^-1. Molar absorptivity value was found to be 4.79 × 10³ L mol^-1 cm^-1. Limits of detection and quantification were found to be as low as 0.04 and 0.13 μg mL^-1. Precision (RSD, 0.4 %) and accuracy (recovery 99.2 ± 0.6 to 101.1 ± 0.8 %) of the developed method were evaluated.

References

K. J. McClellan and B. Jarvis, Lercanidipine: a review of its use in hypertension, Drugs 60 (2000) 1123-1140.
Search in Google Scholar
S. Charde, L. Kumar and R. Saha, Development and validation of high-performance liquid chromatographic method for estimation of lercanidipine in rabbit serum, Anal. Lett. 40 (2007) 2128-2140; DOI: 10.1080/00032710701566636.10.1080/00032710701566636
Search in Google Scholar
I. Popovic, D. Ivanovic, M. Medenica, A. Malenovic and B. Jancic-Stojanovic, LC determination of lercanidipine and its impurities using dry lab software and experimental design procedures, Chromatographia 67 (2008) 449-454; DOI: 10.1365/s10337-008-0536-3.10.1365/s10337-008-0536-3
Search in Google Scholar
P. V. Deore, A. A. Shirkhedkar and S. J. Surana, Simultaneous TLC-densitometric analysis of atenolol and lercanidipine hydrochloride in tablets, Acta Chromatogr. 20 (2008) 463-473; DOI: 10.1556/AChrom.20.2008.3.12.10.1556/AChrom.20.2008.3.12
Search in Google Scholar
Y. Altun, B. Uslu and S. A. Ozkan, Electroanalytical characteristics of lercanidipine and its voltametric determination in pharmaceuticals and human serum on boron-doped diamond electrode, Anal. Lett. 43 (2010) 1958-1975; DOI: 10.1080/00032711003687047.10.1080/00032711003687047
Search in Google Scholar
V. Choudhari, M. V. Suryawanshi, H. R. Mahabal, G. S. Deshchougule, P. K. Bhalerao and S. B. Kuchekar, Simultaneous spectrophotometric estimation of atenolol and lercanidipine hydrochloride in combined dosage form by ratio derivative and dual wavelength method, Int. J. Pharm. Sci. Rev. Res. 3 (2010) 73-76.
Search in Google Scholar
K. S. Acharjya, S. Sahoo, K. K. Dash and M. M. Annapurna, Spectrophotometric determination of lercanidipine hydrochloride in pharmaceutical formulations, Int. J PharmTech Res. 2 (2010) 1431-1436.
Search in Google Scholar
G. Mubeen, D. S. Rao and K. Uvesh, Spectrophotometric method for determination of lercanidipine in tablets, Int. J. ChemTech. Res. 1 (2009) 1186-1188.
Search in Google Scholar
M. A. Abu El-Enin, D. R. El-Wasseef, D. T. El-Sherbiny and S. M. El-Ashry, Spectrophotometric determination of labetalol and lercanidipine in pure form and in pharmaceutical preparations using ferric-1, 10-phenanthroline, Int. J. Biomed. Sci. 5 (2009) 261-266.
Search in Google Scholar
B. Sitaram, S. V. Saradhi and G. D. Rao, Novel spectrophotometric method for estimation of lercanidipine in pharmaceutical formulations, Int. J. Chem. Sci. 7 (2009) 2691-2694.
Search in Google Scholar
K. Tummuru, T. E. Divakar and C. S. P. Sastry, Spectrophotometric determination of some sulphur compounds and tryptophan with brucine and sodium metaperiodate, Analyst 109 (1984) 1105-1106; DOI: 10.1039/AN9840901105.10.1039/an9840901105
Search in Google Scholar
B. S. Furniss, A. J. Hannaford, P. W. G. Smith and A. R. Tatchell, Text Book of Practical Organic Chemistry, 5 th ed., Longman, Harlow 1994, p. 893.
Search in Google Scholar
R. Saha and S. Charde, Development and validation of ultraviolet spectrophotometric method for estimation of lercanidipine hydrochloride in bulk and formulations, AAPS J. 7 (S1) (2005) Abstr. M1001.
Search in Google Scholar
International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonized Tripartite Guidance: Validation of Analytical Procedures: Text and Methodology Q2 (R1), ICH, London 2005.
Search in Google Scholar

Assay of lercanidipine hydrochloride in dosage forms using nucleophilic substitution reaction

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