High-Risk Human Papillomavirus (Hpv) Infection in Women Undergoing in Vitro Fertilization / Okužbe Z Visokorizičnimi Genotipi Humanih Papilomskih Virusov (Hpv) Pri Ženskah, Vključenih V Postopke Zunajtelesne Oploditve

Jančar, Nina; Vrtačnik Bokal, Eda; Poljak, Mario; Oštrbenk, Anja

doi:10.2478/sjph-2013-0017

Abstract

Objective: The aim of our study was to establish the prevalence of high-risk human papillomavirus (hr-HPV) infection in a population of women included in an in vitro fertilisation (IVF) program and to correlate the outcome of IVF cycles with HPV status.

Methods: A total of 195 women undergoing the IVF program were included in the study. A cervical smear for cytological analysis and hr-HPV determination was collected from every woman. RealTime High Risk HPV test (Abbott Molecular Inc., Des Plaines, IL) has been used for the detection of hr-HPV infection. All participants were invited to complete an anonymous questionnaire that included questions regarding medical and sexual history as well as risk factors for HPV infection. HPV 16 and HPV 18 positive women were invited for follow-up gynaecological examinations, including colposcopy 4 to 6 months after the inclusion.

Results: Mean age of included infertile women was 33.7 ± 4.36 years. A total of 16/195 women (8.2%) were hr-HPV positive. Hr-HPV infection was not associated with the percentage of mature oocytes, the percentage of fertilised oocytes, with embryo quality or with pregnancy rate in our study.

Conclusions: The prevalence of hr-HPV genotypes in Slovenian infertile women undergoing IVF is lower than the hr-HPV prevalence in the general population. We were unable to find an association between hr-HPV cervical infection and the outcome of IVF cycles.

References

1. Bouvard V, Baan R, Straif K, Grosse Y, Secretan B, El Ghissassi F et al. A review of human carcinogens - Part B: biological agents. Lancet Oncol 2009; 10: 321-322.10.1016/S1470-2045(09)70096-8
Search in Google Scholar
2. Jancar N, Kocjan BJ, Poljak M, Lunar MM, Bokal EV. Distribution of human papillomavirus genotypes in women with cervical cancer in Slovenia. Eur J Obstet Gynecol Reprod Biol 2009; 145: 184-188.10.1016/j.ejogrb.2009.04.030
Search in Google Scholar
3. Lytwyn A, Sellors JW, Mahony JB, Daya D, Chapman W, Howard M, et al. Adjunctive human papillomavirus testing in the 2-year followup of women with low-grade cervical cytologic abnormalities. Arch Pathol Lab Med 2003; 127: 1169-1175.10.5858/2003-127-1169-AHPTIT
Search in Google Scholar
4. Arbyn M, Paraskevaidis E, Martin-Hirsch P, Prendiville W, Dillner J. Clinical utility of HPV-DNA detection: triage of minor cervical lesions, follow-up of women treated for high-grade CIN: an update of pooled evidence. Gynecol Oncol 2005; 99: S7-S11.10.1016/j.ygyno.2005.07.033
Search in Google Scholar
5. Wright TC Jr, Massad LS, Dunton CJ, Spitzer M, Wilkinson EJ, Solomon D. 2006 American Society for Colposcopy and Cervical Pathology-sponsored Consensus Conference. 2006 consensus guidelines for the management of women with abnormal cervical cancer screening tests. Am J Obstet Gynecol 2007; 197: 346-355.10.1016/j.ajog.2007.07.047
Search in Google Scholar
6. Arbyn M, Anttila A, Jordan J, Ronco G, Schenck U, Segnan N, et al. European guidelines for cervical quality assurance in cervical cancer screening: summary document. 2nd ed. Ann Oncol 2010; 21: 448-458.10.1093/annonc/mdp471
Search in Google Scholar
7. Cox JT. History of the use of HPV testing in cervical screening and in the management of abnormal cervical screening results. J Clin Virol 2009; 45: S3-S12.10.1016/S1386-6532(09)70002-2
Search in Google Scholar
8. Uršič-Vrščaj M, Rakar S, Možina A, Kobal B, Takač I, Deisinger D, et al. Smernice za celostno obravnavo žensk s predrakavimi spremembami materničnega vratu. Ljubljana: Onkološki inštitut, 2011.
Search in Google Scholar
9. Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, et al. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol 2008; 140: 3-11.10.1016/j.ejogrb.2008.03.00918456385
Search in Google Scholar
10. Ochsendorf FR. Sexually transmitted infections: impact on male fertility. Andrologia 2008; 40: 72-75.10.1111/j.1439-0272.2007.00825.x18336453
Search in Google Scholar
11. Lundqvist M, Westin C, Lundkvist Ö, Simberg N, Strand A, Andersson S, et al. Cytologic screening and human papillomavirus test in women undergoing artificial fertilization. Acta Obstet Gynecol Scand 2002; 81: 949-953.10.1034/j.1600-0412.2002.811009.x12366486
Search in Google Scholar
12. Wang Y, Wang C, Qiao J, Wang L, Liang S. Relationship of cytopathology and cervical infection to outcome of in-vitro fertilization embryo transfer. Int J Gynecol Obstet 2008; 101: 21-26.10.1016/j.ijgo.2007.09.035
Search in Google Scholar
13. Tanaka H, Karube A, Kodama H, Fukuda J, Tanaka T. Mass screening for human papillomavirus type 16 infection in infertile couples. J Reprod Med 2000; 45: 907-911.
Search in Google Scholar
14. Spandorfer SD, Bongiovanni AM, Fasioulotis S, Rosenwaks Z, Ledger WJ, Witkin SS. Prevalence o cervical human papillomavirus in women undergoing in vitro fertilization and association with outcome. Fertil Steril 2006; 86: 765-767.10.1016/j.fertnstert.2006.01.051
Search in Google Scholar
15. Almonte M, Silva Idos S, Asare A, Gilham C, Sargent A, Bailey A, et al. Sexual behavior and HPV infection in British women, by postal questionnaires and telephone interviews. J Med Virol 2011; 83: 1238-1246.10.1002/jmv.22085
Search in Google Scholar
16. Huang S, Tang N, Mak WB, Erickson B, Salituro J, Li Y, et al. Principles and analytical performance of Abbott RealTime HR HPV test. J Clin Virol 2009; 45: S13-S17.10.1016/S1386-6532(09)70003-4
Search in Google Scholar
17. Poljak M, Ostrbenk A, Seme K, Ucakar V, Hillemanns P, Bokal EV, et al. Comparison of clinical and analytical performance of the Abbott Realtime High Risk HPV test to the performance of hybrid capture 2 in population-based cervical cancer screening. J Clin Microbiol 2011; 49: 1721-1729.10.1128/JCM.00012-11312264121430098
Search in Google Scholar
18. De Vuyst H, Clifford G, Li N, Franceschi S. HPV infection in Europe. Eur J Cancer 2009; 45: 2632-2639.10.1016/j.ejca.2009.07.01919709878
Search in Google Scholar
19. Učakar V, Poljak M, Klavs I. Pre-vaccination prevalence and distribution of high-risk human papillomavirus (HPV) types in Slovenian women: a cervical cancer screening based study. Vaccine 2012; 30: 116-120.10.1016/j.vaccine.2011.10.06622080175
Search in Google Scholar

High-Risk Human Papillomavirus (Hpv) Infection in Women Undergoing in Vitro Fertilization / Okužbe Z Visokorizičnimi Genotipi Humanih Papilomskih Virusov (Hpv) Pri Ženskah, Vključenih V Postopke Zunajtelesne Oploditve

Abstract

Paradigm

My account