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The Acute Effects of Different Spironolactone Doses on Oxidative Stress in Streptozotocin-Induced Diabetic Rats Cover

The Acute Effects of Different Spironolactone Doses on Oxidative Stress in Streptozotocin-Induced Diabetic Rats

EABR. Experimental and Applied Biomedical Research
Volume 25 (2024): Issue 2 (June 2024)
By: Stefan Simovic,  Aleksandra Vranic,  Petar Ristic,  Jovana Jeremic,  Ivan Srejovic,  Jasna Petrovic,  Vladimir Jakovljevic,  Stefani Bolevich,  Sergey Bolevich and  Vladimir Zivkovic  
Open Access
|May 2021

Figures & Tables

Figure 1.

Parameters of oxidative stress in healthy rats perfused with 0.1 μM of spironolactone compared with diabetic rats perfused with 0.1 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2-) (c) Superoxide anion radical (O2-) (d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.
Parameters of oxidative stress in healthy rats perfused with 0.1 μM of spironolactone compared with diabetic rats perfused with 0.1 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2-) (c) Superoxide anion radical (O2-) (d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.

Figure 2.

Parameters of oxidative stress in healthy rats perfused with 3 μM of spironolactone compared with diabetic rats perfused with 3 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2-) (c) Superoxide anion radical (O2-) (d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.
Parameters of oxidative stress in healthy rats perfused with 3 μM of spironolactone compared with diabetic rats perfused with 3 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2-) (c) Superoxide anion radical (O2-) (d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.

Figure 3.

Parameters of oxidative stress in healthy rats perfused with 0.1 μM of spironolactone compared with healthy rats perfused with 3 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2−) (c) Superoxide anion radical (O2−) (d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.
Parameters of oxidative stress in healthy rats perfused with 0.1 μM of spironolactone compared with healthy rats perfused with 3 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2−) (c) Superoxide anion radical (O2−) (d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.

Figure 4.

Parameters of oxidative stress in diabetic rats perfused with 0.1 μM of spironolactone compared with diabetic rats perfused with 3 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2−) (c) Superoxide anion radical (O2−)(d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.
Parameters of oxidative stress in diabetic rats perfused with 0.1 μM of spironolactone compared with diabetic rats perfused with 3 μM of spironolactone. (a) Hydrogen peroxide (H2O2) (b) Nitrites (NO2−) (c) Superoxide anion radical (O2−)(d) Index of lipid peroxidation (measured as TBARS). Data is presented as mean ± SD. CPP, coronary perfusion pressure.

Percentage of reduction or increase in the each of the parameters of oxidative stress in healthy and diabetic rats treated with different spironolactone doses_

40 cmH2O60 cmH2O80 cmH2O100 cmH2O120 cmH2O
H2O20.1 μM spironolactone healthy vs. control+20.49+1.34+1.30−7.570.00
3 μM spironolactone healthy vs. control+11.12−3.88+0.79−12.62+9.40
0.1 μM spironolactone diabetic vs. diabetic−0.12−11.62−0.28+8.73−8.08
3 μM spironolactone diabetic vs. diabetic−12.22−33.73−37.84−20.12−43.83
NO2−0.1 μM spironolactone healthy vs. control+13.69−10.83−20.12−19.92−31.26
3 μM spironolactone healthy vs. control−2.03−13.64−0.36−4.74+0.90
0.1 μM spironolactone diabetic vs. diabetic+4.12−6.74−4.91+1.26−0.98
3 μM spironolactone diabetic vs. diabetic−3.46−11.98−14.09−9.54−11.25
O2−0.1 μM spironolactone healthy vs. control+5.55−53.58−14.75+41.34−16.96
3 μM spironolactone healthy vs. control+16.83−6.04−16.97−20.30−11.92
0.1 μM spironolactone diabetic vs. diabetic−21.85−3.80+32.66+19.34+67.14
3 μM spironolactone diabetic vs. diabetic+6.31−32.84−25.24−15.17−46.94
TBARS0.1 μM spironolactone healthy vs. control+7.85+1.32−6.91−10.89−9.93
3 μM spironolactone healthy vs. control+7.64+5.60+27.74+28.50+31.63
0.1 μM spironolactone diabetic vs. diabetic+5.25−6.02−7.06+0.60−3.87
3 μM spironolactone diabetic vs. diabetic−13.61−48.17−49.53−46.99−47.15
DOI: https://doi.org/10.2478/sjecr-2021-0025 | Journal eISSN: 2956-2090 | Journal ISSN: 2956-0454
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Language: English
Page range: 103 - 112
Submitted on: Mar 16, 2021
Accepted on: Mar 22, 2021
Published on: May 26, 2021
Published by: University of Kragujevac, Faculty of Medical Sciences
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Spironolactone,
diabetes mellitus,
oxidative stress,
rats
Related subjects:
Medicine,
Clinical medicine,
Clinical medicine, other

© 2021 Stefan Simovic, Aleksandra Vranic, Petar Ristic, Jovana Jeremic, Ivan Srejovic, Jasna Petrovic, Vladimir Jakovljevic, Stefani Bolevich, Sergey Bolevich, Vladimir Zivkovic, published by University of Kragujevac, Faculty of Medical Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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