Table 1.
Relevant characteristics and results of the study group
| Number of patients | 31 |
| Years - mean | 36,33 (29–46) |
| Total number of oocytes | 284 |
| Mean value of oocytes per patient | 9,16 (1–20) |
| Number of fertilized oocytes | 172 (1–11) |
| Number of unfertilized oocytes | 112 (0–15) |
| Fertilization rate | 60,5% |
| Achieved pregnancies (β-HCG>5) | 18 |
| Implantation rate | 31% |
| Pregnancy rate | 58% |
| Mean estradiol concentration in follicular fluid (ng/L) | 1974,65 (115,11–4859,85) |
| Mean value of progesterone concentration in follicular fluid (μg/L) | 11,44 (1,72–20.48) |
| Mean value of testosterone concentration in the follicular fluid (ng/ml) | 5,53 (1,43–13,33) |
Table 2.
Relationship between steroid hormone concentration in follicular fluid and fertilization rate
| Steroid hormones of follicular fluid | Hormone concentrations in follicular fluid (Mean 95%) | ||
|---|---|---|---|
| Normal fertilization (172) | Poor fertilization (112) | p* | |
| Estradiol ng/ml | 1821,79 | 1872,07 | 0,639 |
| Progesterone μg/ml | 10,71 | 11,01 | 0,673 |
| Testosterone ng/ml | 5,27 | 5,13 | 0,956 |
Table 3.
Relationship between fertilization rate and realized pregnancies
| Positive pregnancy outcome | Negative pregnancy outcome | *p | |
|---|---|---|---|
| Fertilization rate (mean value) | 67,34 (±15,15) | 61,31 (±19,22) | 0,379 |
Table 4.
Relationship between in vitro fertilization outcomes and oocyte fertilization methods
| IVF outcome | Метода | p* | |
|---|---|---|---|
| IVF | ICSI | ||
| Positive outcome (β-HCG>5) | 9 (49%) | 10 (51%) | 0,121 |
| Negative outcome (β-HCG<5) | 2 (16,71%) | 10 (83,3) | |
Table 5.
Relationship between mean values of hormone concentrations in follicular fluid and in vitro fertilization outcomes
| Steroid hormones of follicular fluid | Hormone concentrations in follicular fluid (Mean 95%) | ||
|---|---|---|---|
| Positive outcome (β-HCG<5) | Negative outcome (β-HCG>5) | p* | |
| Estradiol ng/L | 1860,27 (115,11–4857,85) | 2133,01 (426,33–4626,82) | 0,540 |
| Progesterone μg/L | 11,28 (1,93–20,48) | 11,67 (1,72–19,56) | 0,859 |
| Testosterone ng/ml | 5,55 (2,51–13,33) | 5,48 (1,43–7,70) | 0,708 |
Table 6.
Relationship between follicular fluid hormone concentration and age, and between in vitro fertilization outcome and age
| Steroid hormones | Mean value of hormone concentration in follicular fluid by age | p* | ||
|---|---|---|---|---|
| 30–34 | 35–39 | 39–42 | ||
| Estradiol (ng/ml) | 1978,97 (957,96–4626,82) | 1981,28 (426,33–4857,85) | 1959,10 (115,11–2817,11) | 0,859 |
| Progesterone (μg/ml) | 10,83 (5,96–15,62) | 10,99 (1,72–20,48) | 12,98 (7,23–17,98) | 0,626 |
| Testosterone (ng/ml) | 5,77 (2,51–13,33) | 5,01 (1,43–7,82) | 5,86 (3,52–7,70) | 0,598 |
| Positive outcome (β-HCG>5) | 8 | 8 | 2 | 0,051 |
| Negative outcome (β-HCG<5) | 4 | 3 | 6 | |