Effect of Various Agents on the Direction of THP-1 Cell Differentiation

Svetlana V. Zubova; Yaroslav V. Radzyukevich; Sergey V. Grachev; Isabela R. Prokhorenko

doi:10.2478/sjecr-2018-0029

Abstract

The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the direction of promonocytic THP-1 cell differentiation was studied.

The differentiating activity of the agents was evaluated by the expression and the ratio of surface receptors (TLR4, CD11b, and CD14) as well as by the change in THP-1 cell phagocytic activity of different degree of differentiation by Flow cytometry.

The THP-1 cell differentiation by VD3 was shown to lead probably to the formation of classical monocytes.

Summarizing we can conclude that VD3 induces the THP-1 cells differentiation with the formation of classical monocytes and the sequence of 1α, 25-dihydroxyvitamin D3 and non-toxic LPS R. capsulatus PG causes the THP-1 cells differentiation with the formation of inflammatory or intermediate monocytes.

References

1. W. Chanput, J.J. Me, H.J. Wichers, THP-1 cell line: An in vitro cell model for immune modulation approach, Int. Immunopharmacology. 23 (2014) 37–45.10.1016/j.intimp.2014.08.002
Search in Google Scholar Back to article
2. S. Tsuchiya, M. Yamabe, Y. Yamaguchi et al., Establishment and characterization of a human acute monocytic leukemia cell line (THP-1), Int. J. Cancer. 26 (1980) 171–176.10.1002/ijc.29102602086970727
Open DOI Search in Google Scholar Back to article
3. G. Pasternak, L. Pasternak, Behavior in vitro of long-term cultured bone marrow or blood cells from chronic myeloid leukemia: adhesion molecules and differentiation antigens as detected by immunocytochemistry, Folia Biologica (Praha). 40 (1994) 439–454.7589702
Search in Google Scholar Back to article
4. K.A. Zarember, P.J. Godowski, Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leucocytes in response to microbes, their products, and cytokines, J. Immunol. 168 (2002) 554–561.10.4049/jimmunol.168.2.55411777946
Search in Google Scholar Back to article
5. R. Huber, D. Pietsch, J. Günther et al., Regulation of monocyte differentiation by specific signaling modules and associated transcription factor networks, Cell. Mol. Life Sci. (2013) Doi: 10.1007/s00018-013-1322-4.2352566510.1007/s00018-013-1322-423525665
Open DOI Search in Google Scholar Back to article
6. Z.C. VanGundy, M. Guerau-de-Arellano M., J.D. Baker et al., Continuous retinoic acid induces the differentiation of mature regulatory monocytes but fails to induce regulatory dendritic cells, BMC Immunology. 15 (2014) 8–22.2454845910.1186/1471-2172-15-8401649924548459
Open DOI Search in Google Scholar Back to article
7. A. Falk, L. Sachs, Clonal regulation of the induction of macrophage- and granulocyte-inducing proteins for normal and leukemic myeloid cells, Int. J. Cancer. 26 (1980) 595–601.697235610.1002/ijc.29102605116972356
Open DOI Search in Google Scholar Back to article
8. S. Kempin, C. Cirrincione, D.S. Straus et al., Improved Remission Rate and Duration in Nodular Non-Hodgkin’s Lymphoma (NNHL) with the Use of Mixed Bacterial Vaccine (MBV), Proc. Am. Soc. Clin. Oncol. 22 (1981) 514.
Search in Google Scholar Back to article
9. M.G. Izban, B.J. Nowicki, S. Nowicki, 1,25-dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells, PLoS ONE. 7 (2012) e49318.10.1371/journal.pone.0049318349591223152895
Search in Google Scholar Back to article
10. Z. Hmama, K.L. Knutson, P. Herrera-Velit et al., Monocyte adherence induced by lipopolysaccharide involves CD14, LFA-1, and cytohesin-1. Regulation by Rho and phosphatidylinositol 3 kinase, J. Biol. Chem. 274 (1999) 1050–1057.10.1074/jbc.274.2.10509873050
Search in Google Scholar Back to article
11. E. Vey, J.H. Zhang, J.M. Dayer, IFN-gamma and 1,25(OH)2D3 induce on THP-1 cells distinct patterns of cell surface antigen expression, cytokine production, and responsiveness to contact with activated T cells, J. Immunol. 149 (1992) 2040–2046.10.4049/jimmunol.149.6.2040
Search in Google Scholar Back to article
12. W. Ma, W. Lim, K. Gee et al., The p38 Mitogen-activated Kinase Pathway Regulates the Human Interleukin-10 Promoter via the Activation of Sp1 Transcription Factor in Lipopolysaccharide-stimulated Human Macrophages, J. Biol. Chem. 276 (2001) 13664–13674.10.1074/jbc.M01115720011278848
Search in Google Scholar Back to article
13. H. Schwende, E. Fitzke, P. Ambs et al., Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3, J. Leukoc. Biol. 59 (1996) 555–561.10.1002/jlb.59.4.555
Search in Google Scholar Back to article
14. Z. Hmama, D. Nandan, L. Sly et al., 1α,25-Dihydroxyvitamin D3-induced myeloid cell differentiation is regulated by a vitamin D receptor-phosphatidylinositol 3-kinase signaling complex, J. Exp. Med. 190 (1999) 1583–1594.10.1084/jem.190.11.1583219573010587349
Search in Google Scholar Back to article
15. M.F. Arnaout, Structure and function of the leukocyte adhesion molecules CD11/CD18, Blood. 75 (1990) 1037–1050.10.1182/blood.V75.5.1037.1037
Search in Google Scholar Back to article
16. C.A. Ambarus, S. Krausz, M. van Eijk et al., Systematic validation of specific phenotypic markers for in vitro polarized human macrophages, J. Immunol. methods. 375 (2012) 196–206.10.1016/j.jim.2011.10.01322075274
Search in Google Scholar Back to article
17. N. Sandor, S. Lukacsi, R.Ungai-Salanki et al., CD11c/CD18 dominates adhesion of human monocytes, macrophages and dendritic cells over CD11b/CD18, PloS ONE. (2016) DOI:10.1371/journal.pone.0163120.10.1371/journal.pone.0163120503346927658051
Open DOI Search in Google Scholar Back to article
18. M. Daigneault, J.A. Preston, H.M. Marriott et al., The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages, PloS ONE. 5 (2010) e8668.10.1371/journal.pone.0008668280019220084270
Search in Google Scholar Back to article
19. S. Gordon, P.R. Taylor, Monocyte and macrophage heterogeneity, Nat. Rev. Immunol. 5 (2005) 953–964.10.1038/nri173316322748
Open DOI Search in Google Scholar Back to article
20. J. Yang, L. Zhang, C. Yu et al., Monocyte and macrophage differentiation: circulation inflammatory monocyte as biomarker for inflammatory diseases, Biomarker research. 2 (2014) 1–9.10.1186/2050-7771-2-1
Search in Google Scholar Back to article
21. J. Cros, N. Cagnard, K. Woollard et al., Human CD14 dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors, Immunity. 33 (2010) 375–386.10.1016/j.immuni.2010.08.012306333820832340
Open DOI Search in Google Scholar Back to article
22. H.W. Ziegler-Heitbrock, G. Fingerle, M. Ströbel et al., The novel subset of CD14+/CD16+ blood monocytes exhibits features of tissue macrophages, Eur. J. Immunol. 23 (1993) 2053–2058.10.1002/eji.18302309027690321
Open DOI Search in Google Scholar Back to article
23. L. Ziegler-Heitbrock, P. Ancuta, S. Crowe et al., Nomenclature of monocytes and dendritic cells in blood, Blood. 116 (2010) e74–e80.10.1182/blood-2010-02-25855820628149
Search in Google Scholar Back to article
24. M. Nahrendorf, F.K. Swirski, Monocyte and macrophage heterogeneity in the heart, Circulation research. 112 (2013) 1624–1633.10.1161/CIRCRESAHA.113.300890375368123743228
Search in Google Scholar Back to article
25. L. Ziegler-Heitbrock, Monocyte subsets in man and other species, Cellular immunology. 289 (2014) 135–139.10.1016/j.cellimm.2014.03.01924791698
Search in Google Scholar Back to article
26. F. Ginhoux, S. Jung, Monocytes and macrophages: developmental pathways and tissue homeostasis, Nat. Rev. Immunol. 14 (2014) 392–404.10.1038/nri367124854589
Open DOI Search in Google Scholar Back to article
27. A. Ohradanova-Repic, C. Machacek, M.B. Fischer et al., Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel, Clinical & translational immunology. 5 (2016) e55.10.1038/cti.2015.39473506126900469
Search in Google Scholar Back to article
28. S.V. Zubova, Y.V. Radzyukevich, I.R. Prokhorenko, The effect of various differentiating agents on the expression of TLR4, CD11b and CD14 receptors of THP-1 cells, Materials of the international conference “Receptors and intracellular signaling” May 22-25 2017, Push-chino, 123-127.
Search in Google Scholar Back to article
29. I.R. Prokhorenko, S.V. Grachev, S.V. Zubova, Y.V. Radziukevich, D.S. Kabanov, D.A. Serov, Lipopolysaccharide of the phototrophic bacterium Rhodobacter capsulatus PG is a factor enhanced the differentiating activity of 1α,25-dihydroxyvitamin D3, Application for invention RU 2016144084/10 20.12.2016.
Search in Google Scholar Back to article
30. Z.K. Makhneva, T.A. Vishnevetskaya, I.R. Prokhorenko, Effect of the isolation method on the yield and composition of lipopolysaccharides from photosynthetic bacteria, Prikl. biochem. microbiol. 32 (1996) 444-447.
Search in Google Scholar Back to article
31. A.D. Friedman, Transcriptional control of granulocyte and monocyte development, Oncogene. 26 (2007) 6816–6828.10.1038/sj.onc.121076417934488
Open DOI Search in Google Scholar Back to article
32. A. Poltorak, I. Smirnova, X. He et al., Genetic and physical mapping of the Lps locus: Identification of the Toll-like 4 receptor as a candidate gene in the critical region, Blood Cells Molecules Dis. 240 (1998) 340–355.10.1006/bcmd.1998.0201
Search in Google Scholar Back to article
33. E. Hailman, H.S. Lichenstein, M.M. Wurfel et al., Lipopolysaccharide (LPS)-binding protein accelerates the binding of LPS to CD14, J. Exp. Med. 179 (1994) 269–277.10.1084/jem.179.1.26921913447505800
Search in Google Scholar Back to article
34. J.-K. Ryu, S.J. Kim, S.-H. Rah et al., Reconstruction of LPS Transfer Cascade Reveals Structural Determinants within LBP, CD14, and TLR4-MD2 for Efficient LPS Recognition and Transfer, Immunity. 46 (2017) 1–13.10.1016/j.immuni.2016.12.01928099858
Search in Google Scholar Back to article
35. C. Han, J. Jin, S. Xu et al., Integrin CD11b negatively regulates TLR-triggered inflammatory responses by activating Syk and promoting degradation of MyD88 and TRIF via Cbl-b, Nat. Immunol. 11 (2010) 734–742.10.1038/ni.190820639876
Open DOI Search in Google Scholar Back to article
36. S. Akira, TLR signaling, Curr. Top. Microbiol. Immunol. 311 (2006) 1–16.10.1007/3-540-32636-7_1
Search in Google Scholar Back to article
37. O. Sharif, V.N. Bolshakov, S. Raines et al., Transcriptional profiling of the LPS induced NF-κB response in macrophages, BMC Immunol. 8 (2007)1–17.10.1186/1471-2172-8-1178146917222336
Search in Google Scholar Back to article
38. N. Mahajan, V. Dhawan, Inhibition of C-reactive protein induced expression of matrix metalloproteinases by atorvastatin in THP-1 cells, Mol. Cell. Biochem. 338 (2010) 77–86.10.1007/s11010-009-0340-x20091096
Search in Google Scholar Back to article
39. J. Schreiber, R.G. Jenner, H.L. Murray et al., Coordinated binding of NF-B family members in the response of human cells to lipopolysaccharide, PNAS. 103, (2006) 5899–5904.10.1073/pnas.0510996103
Search in Google Scholar Back to article
40. A. Moeenrezakhanlou, L. Shephard, L. Lam et al., Myeloid cell differentiation in response to calcitriol for expression CD11b and CD14 is regulated by myeloid zinc finger-1 protein downstream of phosphatidylinositol 3-kinase, J. Leukocyte Biol. 84 (2008) 519–528.10.1189/jlb.120783318495781
Search in Google Scholar Back to article
41. S.Y. Park, S.W. Lee, S.H. Baek et al., Suppression of PU.1-linked TLR4 expression by cilostazol with decrease of cytokine production in macrophages from patients with rheumatoid arthritis, British J. Pharmacology. 168 (2013) 1401–1411.10.1111/bph.12021359664523072581
Search in Google Scholar Back to article
42. M. Rehli, A. Poltorak, L. Schwarzfischer et al., PU.1 and interferon consensus sequence-binding protein regulate the myeloid expression of the human Toll-like receptor 4 genes, J. Biol. Chem. 275 (2000) 9773–9781.10.1074/jbc.275.13.977310734131
Search in Google Scholar Back to article
43. M. Lichtinger, R. Ingram, M. Hornef et al., Transcription factor PU.1 controls transcription start site positioning and alternative TLR4 promoter usage, J. Biol. Chem. 282 (2007) 26874–26883.10.1074/jbc.M70385620017623651
Search in Google Scholar Back to article
44. S. Seuter, A. Neme, C. Carlberg, Epigenomic PU.1-VDR crosstalk modulates vitamin D signaling, BBA. 1860 (2017) 405–415.10.1016/j.bbagrm.2017.02.005
Search in Google Scholar Back to article
45. S. Koschmieder, S. Agrawal, H.S. Radomska et al., Decitabine and Vitamin D3 differentially affect hematopoietic transcription factors to induce monocytic differentiation, Int. J. Oncology. 30 (2007) 349–355.
Search in Google Scholar Back to article

Effect of Various Agents on the Direction of THP-1 Cell Differentiation

Abstract

Paradigm

My account