Skip to main content
Have a personal or library account? Click to login
Genetic identification of wood-destroying fungi in weakened Scots pine (Pinus sylvestris L.) forests using DNA barcoding Cover

Genetic identification of wood-destroying fungi in weakened Scots pine (Pinus sylvestris L.) forests using DNA barcoding

Open Access
|Jun 2026

References

  1. Ageev DV, Bulkonova TM (2025) Dichomitus squalens - Mushrooms of Siberia [online]. Available at <https://mycology.su/dichomitus-squalens.html> [cited 06/09/2025].
  2. Badotti F, de Oliveira FS, Garcia CF, Vaz ABM, Fonseca PLC, Nahum LA, G Oliveira, Goes-Neto A (2017) Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi). BMC Microbiology 17(1):42. https://doi.org/10.1186/s12866-017-0958-x
  3. Begum HA, Ahmad W, Rafiq N, Ali H, Hussain S, Ali B, Ullah I, Ahmed I, Khan B, Khan A (2023) Exploring the pharmacological potential of Trametes hirsuta (White Rot Fungi): Analgesic, anti-Inflammatory, antispasmodic and antimicrobial activities. Pure and Applied Biology 12(2):1183-1193. https://doi.org/10.19045/bspab.2023.120121
  4. Biek D (1984) The mushrooms of northern California. Redding, CA: Spore Prints. ISBN 0-9612020-0-9. OCLC 10870632
  5. BLAST: Basic Local Alignment Search Tool [online]. Available at <https://blast.ncbi.nlm.nih.gov/Blast.cgi> [cited 09/06/2025]
  6. Boczoń A, Hilszczańska D, Wrzosek M, Szczepkowski A, Sierota Z (2021) Drought in the forest breaks plant–fungi interactions. European Journal of Forest Research 140:1301–1321. https://doi.org/10.1007/s10342-021-01409-5
  7. BOLD – The Barcode of Life Data Systems [online]. Available at <https://boldsystems.org> [cited 09/06/2025]
  8. Breitenbach J and F Kranzlin (1984) Fungi of Switzerland. Volume 1: Ascomycetes. Luzern, Switzerland: Verlag Mykologia, 313 p, ISBN-13 978-3856040116
  9. Brichta J, Vacek S, Vacek Z, Cukor J, Mikeska M, Bilek L, Šimůnek V, Gallo J, Brabec P (2023) Importance and potential of Scots pine (Pinus sylvestris L.) in 21st century. Central European Forestry Journal 69(1):3-20. https://doi.org/10.2478/forj-2022-0020
  10. Camarero J, Gazol A, Sanguesa-Barreda G, Vergarechea M, Sanchez RA, Cattaneo N, Vicente-Serrano SM (2021) Tree growth is more limited by drought in rear-edge forests most of the times. Forest Ecosystems 8:25. https://doi.org/10.1186/s40663-021-00303-1
  11. Ćelepirović N, Novak Agbaba S, Karija Vlahović M (2020) DNA barcoding of fungi in the forest ecosystem of the Psunj and Papuk Mountains in Croatia. South- East European forestry 11(2):145-152. https://doi.org/10.15177/seefor.20-17
  12. Cho Y, Kim JS, Dai Y, Gafforov Y, Lim YW (2021) Taxonomic evaluation of Xylodon (Hymenochaetales, Basidiomycota) in Korea and sequence verification of the corresponding species in GenBank. PeerJ 9:e12625. https://doi.org/10.7717/peerj.12625
  13. Das S, Deb B (2015) DNA barcoding of fungi using ribosomal ITS marker for genetic diversity analysis: a review International journal of pure & Applied bioscience 3(3):160-167
  14. Garbelotto M, Gonthier P (2013) Biology, epidemiology, and control of Heterobasidion species worldwide. Annual Review of Phytopathology 51:39-59. https://dx.doi:10.1146/annurev-phyto-082712-102225
  15. GBIF Secretariat (2023). GBIF Backbone Taxonomy. Available at <https://doi.org/10.15468/39omei> [cited 17/06/2025]
  16. Gong S, Ding Y, Wang Y, Jiang G, Zhu C (2018) Advances in DNA barcoding of toxic marine organisms. International Journal of Molecular Sciences 19:2931. https://doi.org/10.3390/ijms19102931
  17. Guo M, Yuan C, Tao L, Cai Y, Zhang W (2022) Life barcoded by DNA barcodes. Conservation Genetics Resources 14:351–365. https://doi.org/10.1007/s12686-022-01291-2
  18. Hallenberg N (1984) A taxonomic analysis of the Sistotrema brinkmannii complex (Corticiaceae, Basidiomycetes). Mycotaxon 21:389-411.
  19. Ji Y, Huotari T, Roslin T, Schmidt NS, Wang J, Yu DWW, Ovaskainen O (2020) Spikepipe: a metagenomic pipeline for the accurate quantification of eukaryotic species occurrences and intraspecific abundance change using DNA barcodes or mitogenomes. Molecular Ecology Resources 20:256–267. https://doi.org/10.1111/1755-0998.13057
  20. Jurc D, Jurc M, Sieber TN, Bojovic S (2000) Endophytic Cenangium ferruginosum (Ascomycota) as a reservoir for an epidemic of Cenangium dieback in Austrian pine. Phyton – Annales Rei Botanicae (Horn, Austria) 40(4):103-108. Available at <https://www.researchgate.net/profile/Thomas-Sieber-2/publication/279662682_Endophytic_Cenangium_ferruginosum_Ascomycota_as_a_reservoir_for_an_epidemic_of_Cenangium_dieback_in_Austrian_pine/links/560d5abf08aeed9d13751f5a/Endophytic-Cenangium-ferruginosum-Ascomycota-as-a-reservoir-for-an-epidemic-of-Cenangium-dieback-in-Austrian-pine.pdf> [cited 17/09/2025]
  21. Kirk PM, Paul F, Cannon D, Minter W, Stalpers JA (2008) Dictionary of the Fungi. CAB International Publishing, 784 p. ISBN-10 0851998267
  22. Koukol O (2012) A new species of Infundichalara from pine litter. Mycotaxon 120:343–352. https://doi.org/10.5248/120.343
  23. Kwaśna H, Mazur A, Łabędzki A, Kuźmiński R, Łakomy P (2016) Communities of fungi in decomposed wood of oak and pine. Forest Research Papers 77:261-275. https://doi.org/10.1515/frp-2016-0028
  24. Lincoff G, Mitchel D (1977) Toxic and hallucinogenic mushroom poisoning. New York: Van Nostrand Reinhold Publishing, 267 p. ISBN-10 0442245807
  25. Lipka ON, Korzukhin MD, Zamolodchikov DG, Dobrolyubov NYu, Krylenko SV, Bogdanovich AYu, Semenov SM (2021) A Role of Forests in Natural Systems Adaptation to Climate Change. Russian Journal of Forest Science (Lesovedenie) 5:531-546. https://doi.org/10.31857/S0024114821050077 (In Russian with English abstract)
  26. Liu SL, He SH, Wang XW, May TW, He G, Chen SL, Zhou LW (2022) Trechisporales emended with a segregation of Sistotremastrales ord. nov. (Basidiomycota). Mycosphere 13(1):862–954. https://dx.doi.org/10.5943/mycosphere/13/1/11
  27. Lopez S, Theelen B, Manserra S, Issak TY, Rytioja J, Makela MR, de Vries RP (2017) Functional diversity in Dichomitus squalens monokaryons. IMA Fungus 8:17–25. https://doi.org/10.5598/imafungus.2017.08.01.02
  28. Musievsky AL (2022) Trends in the dynamics of the forest fund of the Central forest- steppe of the European part of Russia for the period 1966-2017. Proceedings of the Saint Petersburg Forestry Research Institute 2:58-69. https://dx.doi.org/10.21178/2079-6080.2022.2.58 (In Russian with English Abstract)
  29. Nagy LG, Riley R, Tritt A, Adam C, Daum C, Floudas D, Sun H, Yadav JS, Pangilinan J, Larsson K-H, Matsuura K, Barry K, Labutti K, Kuo R, Ohm RA, Bhattacharya SS, Shirouzu T, Yoshinaga Y, Martin FM, Grigoriev IV, Hibbett DS (2016) Comparative genomics of early-diverging mushroom-forming fungi provides insights into the origins of lignocellulose decay capabilities. Molecular Biology and Evolution 33(4):959-970. https://dx.doi:10.1093/molbev/msv337
  30. Neklyaev SE, Larina GE, Seraya LG (2023) Features of active mycogenic xylolysis on Scots pine in the zone of coniferous-deciduous forests. Izvestiya Sankt-Peterburgskoj Lesotexnicheskoj Akademii 244:164-183. DOI: 10.21266/2079-4304.2023.244.164-183 (in Russian with English Abstract)
  31. Nguyen N, Song Z, Bates S, Branco S, Tedersoo L, Menke J, Schilling J, Kennedy P (2016) FUNGuild: an open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology 20:241-248. https://doi.org/10.1016/j.funeco.2015.06.006
  32. Purty RS, Chatterjee S (2016) DNA barcoding: an effective technique in molecular taxonomy. Austin Journal of Biotechnology & Bioengineering 3(1):1059. Available at <https://austinpublishinggroup.com/biotechnology-bioengineering/fulltext/ajbtbe-v3-id1059.php> [cited 25/10/2025]
  33. Rosleskhoz (2024) Rosleskhoz: ploshchadʹ zemelʹ lesnogo fonda za 2023 god vyrosla na 107 tys. ga [Press release]. Federal Forestry Agency of Russia, 21 May 2024. Available at: <https://rosleshoz.gov.ru/news/federal/rosleskhozploshchad-zemel-lesnogo-fonda-za-2023-god-vyrosla-na-107-tysyachgektarov-n10998> [cited 26/10/2025] (In Russian)
  34. Ryu M, Mishra RC, J Jeon, Lee SK, Bae H (2018) Drought-induced susceptibility for Cenangium ferruginosum leads to progression of Cenangium-dieback disease in Pinus koraiensis. Scientific Reports 8:16368. https://dx.doi.org/10.1038/s41598-018-34318-6
  35. Safonov MA, Safonova TI (2023a) Wood-destroying fungi observed on tree and shrub introduced species in the steppe zone of the Southern Urals (Orenburg oblast). Journal of Agriculture and Environment 11(39):1-5. https://doi.org/10.23649/JAE.2023.39.2
  36. Safonov MA, Safonova TI (2023b) Xylotrophic fungi on major forest-forming species in Orenburg oblast. International Research Journal 4(130):1-6. https://doi.org/10.23670/IRJ.2023.130.66 (In Russian with English Abstract)
  37. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, List E, Bolchacova K, Voigt PW, Crous AN, Miller MJ, Wingfield MC, Aime K, An F, Bai RW, Barreto D and D. Schindel (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proceedings of the National Academy of Sciences of the United States of America 109(16):6241-6246, https://doi.org/10.1073/pnas.1117018109 (2012)
  38. Shen S, Zhang X, Jian S (2024) The distributional range changes of European Heterobasidion under future climate change. Forests 15(11):1863. https://dx.doi.org/10.3390/f15111863
  39. Space Observatory: Forests of Russia from space [online]. Available at http://carbon.cepl.rssi.ru/maps> [cited 06/09/2025]
  40. Spirin V, Volobuev S, Viner I, Miettinen O (2021) On Sistotremastrum and similar-looking taxa (Trechisporales, Basidiomycota). Mycological Progress 20:453–476. https://doi.org/10.1007/s11557-021-01682-z
  41. Stokland J, Larsson K-H (2011) Legacies from natural forest dynamics: different effects of forest management on wood-inhabiting fungi in pine and spruce forests. Forest Ecology and Management (261):707-1721. https://dx.doi.org/10.1016/j.foreco.2011.01.003
  42. Storozhenko VG (2015) The structure of tree stands and wood-destroying fungi of native pine biogeocoenoses of the Russian plain. Siberian Forest Journal 4:30-39. https://dx.doi.org/10.15372/SJFS20150403 (In Russian with English Abstract)
  43. Zamolodchikov D, Shvidenko A, Bartalev S, Kulikova E, Held A, Valentini R, Lindner M (2020) State of Russian forests and forestry. Chapter 2 in: Leskinen P, Lindner M, Verkerk PJ, Nabuurs GJ, Van Brusselen J, Kulikova E, Hassegawa M, Lerink B (eds.) Russian forests and climate change. What Science Can Tell Us 11. European Forest Institute, p. 21. https://doi.org/10.36333/wsctu11
  44. Telleria M, Duenas D, Martina M (2014) Sistotremastrum chilensis (Trechisporales, Basidiomycota), a new species from Chilean Patagonia. Phytotaxa 158(1):93–98. https://doi.org/10.11646/phytotaxa.158.1.7
  45. Telleria M, Melo I, Duenas M, Salcedo I (2013) Sistotremastrum guttuliferum: a new species from the Macaronesian islands. Mycological Progress 12:687–692. https://doi.org/10.1007/s11557-012-0876-0
  46. Trudell S, Ammirati J (2009) Mushrooms of the Pacific Northwest. Timber Press Field Guides. Portland, OR: Timber Press, p. 264. https://doi.org/10.1016/j.enzmictec.2006.09.015
  47. Valentini A, Pompanon F, Taberlet P (2009) DNA barcoding for ecologists. Trends in Ecology and Evolution 24:110-7. https://dx.doi.org/10.1016/j.tree.2008.09.011
  48. Varentsova EY, Mamaev NA, Martirova MB (2023) Scots pine and Norway spruce phytopathological condition in forest stands of the Leningrad region. Izvestia Sankt-Peterburgskoj Lesotexnicheskoj Akademii 244:131-149. https://doi.org:10.21266/2079-4304.2023.244.131-149 (In Russian with English Abstract)
  49. Wu W, Diao Y (2023) The chalara‑like anamorphs of Leotiomycetes. Fungal Diversity 119:213–490. https://doi.org/10.1007/s13225-023-00515-6
  50. Xu J (2016) Fungal DNA barcoding. Genome 59(11):913-932. https://doi.org/10.1139/gen-2016-0046
  51. Zmitrovich I (2004) Genus Athelia Pers. in Russia. Turczaninowia 7 (4):22-46. (In Russian)
  52. Zmitrovich I, Volobuev S, Dudka V, Zhukova E, Sidelnikova M, Bondartseva M (2019) Ganoderma applanatum (Polyporales, Basidiomycota) at the Saint Petersburg area. Mycology and Phytopathology (Mikologiya i Fitopatologiya) 53(6):354-362. https://doi.org/10.1134/s0026364819060084
DOI: https://doi.org/10.2478/sg-2026-0007 | Journal eISSN: 2509-8934 | Journal ISSN: 0037-5349
Language: English
Page range: 54 - 65
Published on: Jun 2, 2026
In partnership with: Paradigm Publishing Services

© 2026 Аnna А. Popova, Mikhail Yu. Syromyatnikov, Igor V. Lykov, Maria I. Gladkikh, Ekaterina Y. Nesterova, Оlga А. Fedorova, Konstantin V. Krutovsky, published by Johann Heinrich von Thünen Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.