Stock plant etiolation and immersion in IBA solution stimulate rooting capacity of Pinus patula cuttings
References
- Abdel-Mohsen MAA, Rashedy AAH (2023) Stock plant etiolation reduces rooting of sub-terminal olive cuttings by reducing total sugars, IAA, indole/phenol ratio, and IAA/GA ratio. Acta Physiologiae Plantarum 45(9): 104. DOI: https://doi.org/10.1007/s11738-023-03582-z
- Agulló-Antón MÁ, Ferrández-Ayela A, Fernández-García N, Nicolás C, Albacete A, Pérez-Alfocea F, Sánchez-Bravo J, Pérez-Pérez JM, Acosta M (2014) Early steps of adventitious rooting: Morphology, hormonal profiling and carbohydrate turnover in carnation stem cuttings. Physiologia Plantarum 150(3): 446–462. DOI: https://doi.org/10.1111/ppl.12114
- Agulló-Antón MÁ, Sánchez-Bravo J, Acosta M, Druege U (2011) Auxins or sugars: what makes the difference in the adventitious rooting of stored carnation cuttings? Journal of Plant Growth Regulation 30(1): 100–113. DOI: https://doi.org/10.1007/s00344-010-9174-8
- Aloni R. 2013. Role of hormones in controlling vascular differentiation and the mechanism of lateral root initiation. Planta 238(5): 819–830. DOI: https://doi.org/10.1007/s00425-013-1927-8
- Apine I, Freidenfelds K, Megre D, Dokane K, Kondratovics U. (2013) The effect of stock plant etiolation on rooting and overwinter survival of Deciduous azalea cuttings. Acta Horticultura 990: 465-472. DOI: https://doi.org/10.17660/ActaHortic.2013.990.60
- Araujo-Viera de Souza JC, Bender AG, Reutemann AG, Perreta MG, Córdoba MS, Tivano JC, Barroso DG, Gariglio NF, Mroginski LA, Vegetti AV. (2021) Influencia del grado de lignificación de los propágulos de jardín y minijardín clonal en el enraizamiento de estacas y miniestacas de Prosopis alba Griseb. Re-vista FAVE-Ciencias Agrarias 20(1): 287–304.
- Basuchaudhuri P. (2021) Auxins in rooting of cuttings. Indian Journal of Plant Sciences 10: 69–85. http://www.cibtech.org/jps.htm
- Bautista-Ojeda GI, Vargas-Hernández JJ, Jiménez-Casas M, López-Peralta MCG. (2022) Manejo de planta y aplicación de IBA en el enraizado de estacas de Pinus patula. Madera y Bosques 28(1): 1–11.
- Bhatla SC, Lal MA. (2023) Plant Physiology, Development and Metabolism (Second Edition). Springer Nature.
- Blommaert KLJ. (1954) Growth and inhibit substances in relation to the rest period of the potato tuber. Nature 174: 970–972. DOI: https://doi.org/10.1038/174970b0
- da Costa CT, de Almeida MR, Ruedell CM, Schwambach J, Maraschin FS, Fett-Neto AG. (2013) When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings. Frontiers in Plant Science 4: 133. DOI: https://doi.org/10.3389/fpls.2013.00133
- Damodaran S, Strader LC. (2019) Indole 3-butyric acid metabolism and transport in Arabidopsis thaliana. Frontiers in Plant Science 10: 851. DOI: https://doi.org/10.3389/fpls.2019.00851
- de Almeida MR, Schwambach J, Silveira V, Heringer AS, Fett JP, Fett-Neto AG. (2020) Proteomic profiles during adventitious rooting of Eucalyptus species relevant to the cellulose industry. New Forests 51(2): 213–241. DOI: https://doi.org/10.1007/s11056-019-09728-7
- Druege U, Hilo A, Pérez-Pérez JM, Klopotek Y, Acosta M, Shahinnia F, Zerche S, Franken P, Hajirezaei MR. (2019) Molecular and physiological control of adventitious rooting in cuttings: Phytohormone action meets resource allocation. Annals of Botany 123(6): 929–949. DOI: https://doi.org/10.1093/aob/mcy234
- Druege U, Kadner R. (2008) Response of post-storage carbohydrate levels in pelargonium cuttings to reduced air temperature during rooting and the relationship with leaf senescence and adventitious root formation. Postharvest Biology and Technology 47(1): 126–135. DOI: https://doi.org/10.1016/j.postharvbio.2007.06.008
- Dumroese RK, Luna T, Landis TD (Eds.) (2009) Nursery manual for native plants: a guide for tribal nurseries. US Department of Agriculture, Forest Service.
- Dvorak, W.S.; Hodge, G.R.; Kietzka, J.E.; Malan, F.; Osorio, L.F.; Stanger, T.K. Pinus patula. In Conservation and Testing of Tropical & Subtropical Forest Tree Species; The CAMCORE Cooperative-College of Natural Resources, NCSU: Raleigh, NC, USA, 2000; pp. 149–173.
- Eliyahu A, Duman Z, Sherf S, Genin O, Cinnamon Y, Abu-Abied M, Weinstain R, Dag A, Sadot E. (2020) Vegetative propagation of elite Eucalyptus clones as food source for honeybees (Apis mellifera); adventitious roots versus callus formation. Israel Journal of Plant Sciences 67(1–2): 83–97. DOI: https://doi.org/10.1163/22238980-20191112
- Elmongy MS, Cao Y, Zhou H, Xia Y. (2018) Root development enhanced by using indole-3-butyric acid and naphthalene acetic acid and associated biochemical changes of in vitro Azalea Microshoots. Journal of Plant Growth Regulation 37(3): 813–825. DOI: https://doi.org/10.1007/s00344-017-9776-5
- Escamilla-Hernández N, Aldrete A, Vargas-Hernández JJ, López-López MÁ. (2020) Propagación vegetativa de Pinus patula Schiede ex Schltdl. et Cham. en diferentes sustratos. Revista Fitotecnia Mexicana 43(2): 215. DOI: https://doi.org/10.35196/rfm.2020.2.215
- Evert RF. (2006) Esau’s plant anatomy. Meristems, cells, and tissues of the plant body: their structure, function, and development (Third Edition). John Wiley & Sons.
- Frick EM, Strader LC. (2018) Roles for IBA-derived auxin in plant development. Journal of Experimental Botany 69(2): 169–177. DOI: https://doi.org/10.1093/jxb/erx298
- Geiss G, Gutierrez L, Bellini C. (2009) Adventitious root formation: New insights and perspectives. Annual Plant Reviews: Root Development 37: 127–156 Gonin M, Bergougnoux V, Nguyen TD, Gantet P, Champion A. (2019) What makes adventitious roots? Plants 8(7): 240. DOI: https://doi.org/10.3390/plants8070240
- Hamann A. (1998) Adventitious root formation in cuttings of loblolly pine (Pinus taeda L): developmental sequence and effects of maturation. Trees - Structure and Function 12: 175–180.
- Harshita GT, Singh G. (2022) The use of auxin and media for rooting of citrus stem cuttings: A review. The Pharma Innovation Journal 11(3): 1268–1273. http://www.thepharmajournal.com
- Hartmann H, Kester D, Davies F, Geneve R. (2014) Plant propagation: Principles and practices. Prentice Hall. DOI: https://doi.org/10.1016/0305-1978(90)90018-B
- Henrique A, Campinhos EN, Ono EO, De Pinho SZ. (2006) Effect of plant growth regulators in the rooting of Pinus cuttings. Brazilian Archives of Biology and Technology 49(2): 189–196. DOI: https://doi.org/10.1590/S1516-89132006000300002
- Husen A. (2008) Stock-plant etiolation causes drifts in total soluble sugars and anthraquinones and promotes adventitious root formation in teak (Tectona grandis L. f.) coppice shoots. Plant Growth Regulation 54(1): 13–21. DOI: https://doi.org/10.1007/s10725-007-9222-y
- Kanmegne G, Mbouobda HD, Fotso Mbakop CN, Omokolo DN. (2017) Influence of stockplant fertilization on tissue concentrations of nitrogen, carbohydrates and amino acids and rooting of leafy stem cuttings of Cola anomala K. Schum (Malvaceae). New Forests 48(1): 17–31. DOI: https://doi.org/10.1007/s11056-016-9553-5
- Korasick DA, Enders TA, Strader LC. (2013) Auxin biosynthesis and storage forms. Journal of Experimental Botany 64(9): 2541–2555. DOI: https://doi.org/10.1093/jxb/ert080
- Kreiser M, Giblin C, Murphy R, Fiesel P, Braun L, Johnson G, Wyse D, Cohen JD. 2016. Conversion of Indole-3-Butyric Acid to Indole-3-Acetic Acid in shoot tissue of Hazelnut (Corylus) and Elm (Ulmus). Journal of Plant Growth Regulation 35(3): 710–721. DOI: https://doi.org/10.1007/s00344-016-9574-5
- Legué V, Rigal A, Bhalerao RP. (2014) Adventitious root formation in tree species: Involvement of transcription factors. Physiologia Plantarum 151(2): 192–198. DOI: https://doi.org/10.1111/ppl.12197
- Lira G DE. (2020) Guía Básica de Pinos Mexicanos. Boskia: México. Loewus FA. (1952) Improvement in anthrone method for determination of carbohydrates. Analytical Chemistry 24(1): 219.
- Ludwig-Müller J. (2000) Indole-3-butyric acid in plant growth and development. Plant Growth Regulation 32: 219–230.
- Majada J, Martínez-Alonso C, Feito I, Kidelman A, Aranda I, Alía R. (2011) Mini-cuttings: An effective technique for the propagation of Pinus pinaster Ait. New Forests 41(3): 399–412. DOI: https://doi.org/10.1007/s11056-010-9232-x
- Pacholczak A, Jędrzejuk A, Sobczak M. (2017) Shading and natural rooting biostimulator enhance potential for vegetative propagation of dogwood plants (Cornus alba L.) via stem cuttings. South African Journal of Botany 109: 34–41. DOI: https://doi.org/10.1016/j.sajb.2016.12.009
- Pacholczak A, Szydło W, Łukaszewska A. (2005) The effect of etiolation and shading of stock plants on rhizogenesis in stem cuttings of Cotinus coggygria. Acta Physiologiae Plantarum 27(4): 417–428. DOI: https://doi.org/10.1007/s11738-005-0046-y
- Pacurar DI, Perrone I, Bellini C. (2014) Auxin is a central player in the hormone cross-talks that control adventitious rooting. Physiologia Plantarum 151(1): 83-96. DOI: https://doi.org/10.1111/ppl.12171
- Pan X, Welti R, Wang X. (2010) Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry. Nature Protocols 5(6): 986–992. DOI: https://doi.org/10.1038/nprot.2010.37
- Park Y S. (2002) Implementation of conifer somatic embryogenesis in clonal forestry: technical requirements and deployment considerations. Annals of Forest Science, 59(5-6): 651-656. DOI: https://doi.org/10.1051/forest:2002051
- Pizarro A, Díaz-Sala C. (2020) Effect of polar auxin transport and gibberellins on xylem formation in pine cuttings under adventitious rooting conditions. Israel Journal of Plant Sciences 67(1–2): 27–39. DOI: https://doi.org/10.1163/22238980-20191120
- Riov J, Foxa H, Attiasa R, Shklar G, Farkash-Haim L, Sitbon R, Moshe Y, Abu-Abied M, Sadot E, David-Schwartz R. (2020) Improved method for vegetative propagation of mature Pinus halepensis and its hybrids by cuttings. Israel Journal of Plant Sciences 67(1–2): 5–15. DOI: https://doi.org/10.1163/22238980-20191118
- Rivera-Rodríguez M, Vargas-Hernández JJ, López-Upton J, Villegas-Monter Á, Jiménez-Casas M. (2016) Enraizamiento de estacas de Pinus patula. Revista Fitotecnia Mexicana 39(4): 385-392. DOI: https://doi.org/10.35196/rfm.2020.2.215
- Statical Analysis System. (2013) SAS 9.4 [Computer software]. Institute Inc. NC USA. 5121p
- Steffens B, Rasmussen A. (2016) The physiology and molecular biology of adventitious root formation. Plant Physiology 170(2): 603–617. DOI: https://doi.org/10.1104/pp.15.01360
- Strader LC, Bartel B. (2011) Transport and metabolism of the endogenous auxin precursor indole-3-butyric acid. Molecular Plant 4(3): 477–486. DOI: https://doi.org/10.1093/mp/ssr006
- Wei H, Zhao H, Chen X, He X. (2020) Secondary metabolites, carbohydrate accumulation, and nutrient uptake in Aralia elata (Miq.) Seem seedlings exposed to shoot cutting and different LED spectra. Acta Physiologiae Plantarum 42(11):162. DOI: https://doi.org/10.1007/s11738-020-03149-2
- White, T. L., Adams, W. T., & Neale, D. B. (Eds.). (2007). Forest genetics. Cabi. Wright, J. A., Osorio, L. F., & Dvorak, W. S. (1996). Realised and predicted genetic gain in the Pinus patula breeding program of Smurfit Carton de Colombia. South African Forestry Journal, 175(1), 19-22.
- Zavaleta-Mancera HA, Engleman EM. (1994) Anatomy of the ovule and seed of Manilkara zapota (L.) Van Royen (Sapotaceae). Phytomorphology 44: 169–175.
- Zerche S, Haensch KT, Druege U, Hajirezaei MR. (2016) Nitrogen remobilisation facilitates adventitious root formation on reversible dark-induced carbohydrate depletion in Petunia hybrida. BMC Plant Biology 16(1): 219. DOI: https://doi.org/10.1186/s12870-016-0901-6
- Zhou Y, Wei K, Hao X, Wang L, Li N, Zhang W, Tang S, Li D, Zhang W. (2025) Differential rooting efficacy of growth regulators in Camellia sinensis Cuttings: A physiological and biochemical analysis. Horticulturae 11(3): 289. DOI: https://doi.org/10.3390/horticulturaT11030289
Language: English
Page range: 66 - 75
Published on: Aug 8, 2026
Published by: Johann Heinrich von Thünen Institute
In partnership with: Paradigm Publishing Services
Related subjects:
© 2026 Georgina Irasema Bautista-Ojeda, J. Jesús Vargas-Hernández, Marcos Jiménez-Casas, Arnulfo Aldrete, María Cristina Guadalupe López-Peralta, F. Víctor Conde-Martínez, e Hilda Araceli Zavaleta-Mancera, published by Johann Heinrich von Thünen Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.