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Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae) Cover

Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)

By: Zhaohong Ding and  Jianbo Lu  
Open Access
|Mar 2019

Abstract

Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.

DOI: https://doi.org/10.2478/sg-2019-0005 | Journal eISSN: 2509-8934 | Journal ISSN: 0037-5349
Language: English
Page range: 26 - 28
Published on: Mar 26, 2019
Published by: Johann Heinrich von Thünen Institute
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2019 Zhaohong Ding, Jianbo Lu, published by Johann Heinrich von Thünen Institute
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.