Pratylenchus neglectus (Nematoda: Pratylenchidae) under the rhizosphere of Brassica napus

S. Kumari

doi:10.2478/s11687-012-0019-9

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Pratylenchus neglectus (Nematoda: Pratylenchidae) under the rhizosphere of Brassica napus

Helminthologia

Volume 49 (2012): Issue 2 (June 2012)

By: S. Kumari

Open Access

|Apr 2012

Abstract

Soil samples under the rhizosphere of Brasicca napus were collected from three localities (Bílé Podolí, Prague, Kylešovice). Two localities Prague and Kylešovice were positive for the presence of Pratylenchus neglectus. The species was identified using morphological features and the morphological identification was verified by using published species-specific primers and by sequencing 18S and 28S genes of ribosomal DNA.

References

[1] Al-Banna, L., Ploeg, A. T., Williamson, V. M., Kaloshian, I. (2004): Discrimination of six Pratylenchus species using PCR and species-specific primers. J. Nematol., 36: 142–146
Search in Google Scholar
[2] Andrásy, I. (2007): Free-living nematodes of hungary (Nematoda Errantia). II. Pedozoologica Hungarica, Budapest.
Search in Google Scholar
[3] Castillo, P., Vovlas, N. (2008): Pratylenchus (Nematoda, Pratylenchidae): Diagnosis, biology, pathogenicity and management. Nematol. Monogr. Perspect., 6: 1–530
Search in Google Scholar
[4] de Ley, P., Félix, M. A., Frisse, L. M., Nadler, S. A., Sternberg, P. W., Thomas, W. K. (1999): Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1: 591–612 http://dx.doi.org/10.1163/15685419950855910.1163/156854199508559
Search in Google Scholar
[5] Holterman, M., Karssen, G., van Den Elsen, S., van Megen, H., Bakker, J., Helder, J. (2009): Small subunit rDNA-based phylogeny of the Tylenchida sheds light on relationships among some high-impact plant-parasitic nematodes and the evolution of plant feeding. Phytopathology, 99: 227–235. DOI: 10.1094/PHYTO-99-3-0227 http://dx.doi.org/10.1094/PHYTO-99-3-022710.1094/PHYTO-99-3-022719203274
Search in Google Scholar
[6] Holterman, M., van Der Wurff, A., van Den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J., Helder, J. (2006): Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Mol. Biol. Evol., 23: 1792–1800. DOI: 10.1093/molbev/mls044 http://dx.doi.org/10.1093/molbev/msl044
Search in Google Scholar
[7] Jatala, P., Bridge, J. (1990): Nematode parasites of root and tuber crops. In: Luc, M., Sikora, R. A., and Bridge, J. (eds), Plant parasitic nematodes in subtropical and tropical agriculture, 137–180. CABI, Wallingford, UK.
Search in Google Scholar
[8] Lasserre, F., R. Rivoal, R. Cook (1994): Interactions between Heterodera avenae and Pratylenchus neglectus on wheat. J. Nematol., 26: 336–344
Search in Google Scholar
[9] Nicol, J., R. Rivoal, S. Taylor, M. Zaharieva (2004): Global importance of cyst (Heterodera spp.) and lesion nematodes (Pratylenchus spp.) on cereals: distribution, yield loss, use of host resistance and integration of molecular tools. Nematol. Monogr. Perspect., 2, 233–251. 10.1163/9789004475236_027
Search in Google Scholar
[10] Smiley, R. W., Machado, S. (2009): Pratylenchus neglectus reduces yield of winter wheat in dryland cropping systems. Plant Dis., 93: 263–271. DOI: 10.1094/PDIS- 93-3-0263 http://dx.doi.org/10.1094/PDIS-93-3-0263
Search in Google Scholar
[11] Smiley, R. W., Whittaker, R. G., Gourlie, J. A., Easley, S. A. (2005): Suppression of wheat growth and yield by Pratylenchus neglectus in the Pacific Northwest. Plant Dis., 89: 958–968. DOI: 10.1094/PD-89-0958 http://dx.doi.org/10.1094/PD-89-095810.1094/PD-89-095830786629
Search in Google Scholar
[12] Stanton, J. M., Mcnicol, C. D., Steele, V. (1998): Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australas. Plant Path., 27: 112–115. DOI: 10.1071/AP98014 http://dx.doi.org/10.1071/AP9801410.1071/AP98014
Search in Google Scholar
[13] Subbotin, S. A., Ragsdale, E. J., Mullens, T., Roberts, P. A., Mundo-ocampo, M., Baldwin, J. G. (2008): A phylogenetic framework for root lesion nematodes of the genus Pratylenchus (Nematoda): Evidence from 18S and D2-D3 expansion segments of 28S ribosomal RNA genes and morphological characters. Mol. Phylogenet. Evol., 48: 491–505. DOI: 10.1016/j.ympev.2008.04.028 http://dx.doi.org/10.1016/j.ympev.2008.04.02810.1016/j.ympev.2008.04.02818514550
Search in Google Scholar
[14] Taylor, S. P., Vanstone, V. A., Ware, A. H., Mckay, A. C., Szot, D., Russ, M. H. (1999): Measuring yield loss in cereals caused by root lesion nematodes (Pratylenchus neglectus and P. thornei) with and without nematicide. Aust. J. Agric. Res., 50: 617–622. DOI: 10.1071/A98103 http://dx.doi.org/10.1071/A9810310.1071/A98103
Search in Google Scholar
[15] Webb, R. M. (1990): Effects of the nematode Pratylenchus fallax on roots of oilseed rape (Brassica napus var. oleifera). Rev. Nématol., 13: 115–117
Search in Google Scholar
[16] Webb, R. M. (1996): In vitro studies of six species of Pratylenchus (Nematoda: Pratylenchidae) on four cultivars of oilseep rape (Brassica napus var. oleifera). Nematologica, 42: 89–95 http://dx.doi.org/10.1163/187529296X0009410.1163/187529296X00094
Search in Google Scholar
[17] Yan, G. P., Smiley, R. W., Okubara, P. A., Skantar, A., Easley, S. A., Sheedy, J. G., Thompson, A. L (2008): Detection and discrimination of Pratylenchus neglectus and P. thornei in DNA extracts from soil. Plant Dis., 92: 1480–1487. DOI: 10.1094/PDIS-92-11-1480 http://dx.doi.org/10.1094/PDIS-92-11-148010.1094/PDIS-92-11-148030764446
Search in Google Scholar

Articles in this issue

DOI: https://doi.org/10.2478/s11687-012-0019-9 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

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Language: English

Page range: 92 - 95

Published on: Apr 11, 2012

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Pratylenchus neglectus,

PCR,

Related subjects:

Zoology,

Ecology,

Life sciences, other,

Medicine,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2012 S. Kumari, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 49 (2012): Issue 2 (June 2012)