An endemic area of Dirofilaria immitis seropositive dogs at the eastern foothills of Mt Olympus, Northern Greece

Lefkaditis, M.; Koukeri, S.; Cozma, V.

doi:10.2478/s11687-010-0001-3

Abstract

Following an examination conducted on a population of three hundred and forty-one dogs living on the eastern foothills of Mt Olympus in Northern Greece, 17.9 % were found to be seropositive for the Dirofilaria immitis specific antigen. These results indicate that the region is an endemic area for the above-mentioned parasitosis in dogs. Serum samples were examined using the PetChek kit Snap (IDDEX Laboratories, ELISA, Portland USA) according to the manufacturer’s instructions.

With respect to gender, age and type of coat, the statistical analysis has shown that the prevalence of seropositive results is significantly higher (P < 0.05) among male dogs, older dogs and short-haired dogs. As regards breed and size, the same study has recorded a higher prevalence in mixed breed and small-sized dogs.

References

[1] Anon (1997): American Heartworm Society recommended procedures for the diagnosis, prevention and management of heartworm (Dirofilaria immitis) infection in dogs. In Heartworm Symp. Proc. Canine Pract., 22: 8–15
Search in Google Scholar
[2] Aranda, C., Octavi, P., Eritja, R., Castella, J. (1998): Canine filariasis. Importance and transmission in the Baix Llobregat area, Barcelona (Spain). Vet. Parasitol., 77: 267–275. DOI: 10.1016/S0304-4017(98)00109-5 http://dx.doi.org/10.1016/S0304-4017(98)00109-510.1016/S0304-4017(98)00109-5
Search in Google Scholar
[3] Araujo, A. M. (1996): Canine and human Dirofilaria immitis infections in Portugal. A review. In Proc. VIIth EMOP. Parassitologia, 38(Suppl. 1–2), 366–371
Search in Google Scholar
[4] Bailey, T. S., Sohrabi, A., Roberts, S. S. (1990): Pulmonary coin lesions caused by Dirofilaria immitis. J. Surg. Oncol., 44: 268–272. DOI: 10.1002/jso.2930440415 http://dx.doi.org/10.1002/jso.293044041510.1002/jso.2930440415
Search in Google Scholar
[5] Bowman, D. D. (2003): Parasitology for veterinarians. Philadelphia, U. S. A., W. B. Saunders Company, 414 pp.
Search in Google Scholar
[6] Brglez, J., Senk, L. (1987): Dirofilaria immitis (Leidy, 1856) Railliet et Henry, 1911, in dog. In Zbor. Bioteh. Fak. Univ. E. Kardelja Ljubljani. Veterinarstvo, 24: 69–72
Search in Google Scholar
[7] Bucklar, H., Scheu, U., Mossi, R., Deplazes, P. (1998): Is dirofilariosis in dogs spreading in south Switzerland? Schweiz. Arch. Tierheilkd., 140: 255–260
Search in Google Scholar
[8] Calvert, C. A., Mandell, C. P. (1982): Diagnosis and management of feline heartworm disease. J. Am. Vet. Med. Assoc., 180: 550–552
Search in Google Scholar
[9] Courtney, C. H., Zeng, Q. Y. (2001): Comparison of heartworm antigen test kit performance in dogs having low heartworm burdens. Vet. Parasitol., 96: 317–322. DOI: 10.1016/S0304-4017(01)00374-0 http://dx.doi.org/10.1016/S0304-4017(01)00374-010.1016/S0304-4017(01)00374-0
Search in Google Scholar
[10] Cringoli, G., Rinaldi, L., Veneziano, V., Capelli, G. (2001): A prevalence survey and risk analysis of filariosis in dogs from the Mt. Vesuvius area of southern Italy. Vet. Parasitol., 102: 243–252. DOI: 10.1016/S0304-4017(01)00529-5 http://dx.doi.org/10.1016/S0304-4017(01)00529-510.1016/S0304-4017(01)00529-5
Search in Google Scholar
[11] Diaku, A. (2001): The prevalence of canine dirofilariosis in the region of Attiki. Bull. Hellenic. Vet. Med. Soc., 52: 152–156
Search in Google Scholar
[12] Doby, J. M., Guiguen, C., Lefevre, R. (1986): Présence de Dirofilaria immitis (Leidy, 1856) chez le chien en Bretagne. Bull. Soc. Franc. Parasitol., 4: 51–54
Search in Google Scholar
[13] Doganay, A., Biyikoglu, G. (1992): The Filarial Nematodes in dogs. Etlik. Vet. Mikrob. Derg., 7: 127–139 (In Turkish)
Search in Google Scholar
[14] Fan, C. K., Su, K. E., Lin, Y. H., Liao, C. W., Du, W. Y., Chiou, H. Y. (2001): Seroepidemiologic survey of Dirofilaria immitis infection among domestic dogs in Taipei City and mountain aboriginal districts in Taiwan (1998–1999). Vet. Parasitol., 102: 113–120. DOI: 10.1016/S0304-4017(01)00511-8 http://dx.doi.org/10.1016/S0304-4017(01)00511-810.1016/S0304-4017(01)00511-8
Search in Google Scholar
[15] Founta, A., Theodoridis, Y., Frydas, S., Chliounakis, S. (1999): The presence of filarial parasites of dogs in Serrae province. Bull. Hellenic. Vet. Med. Soc., 50: 315–320
Search in Google Scholar
[16] Genchi, C., Solari Bazano, F., Bandi, C., di Sacco, B., Venco, L., Vezzoni, A., Cancrini, G. (1995): Factors influencing the spread of heartworm in Italy; interaction between Dirofilaria immitis and Dirofilaria repens. In: Soll, M. D., Knight, D. H. (Eds) Proc. Heartworm Symp. ′95, American Heartworm Society, Batavia., IL: 65–71
Search in Google Scholar
[17] Genchi, C., Solari Basano, F., Marrone, R. V., Petruschke, G. (1998): Canine and feline heartworm infection with special emphasis on Italy. In: Soll, M. D., Knight, D. H. (Eds) Proc. Heartworm Symp. ′98, American Heartworm Society, Batavia: 75–82
Search in Google Scholar
[18] Georgieva, D. A., Ivanov, A. I., Prelesov, P. N. (1999): Studies on the parasitic fauna in stray dogs in the Stara Zagora region. Bulgarian J. Vet. Med., 2: 121–124
Search in Google Scholar
[19] Glickman, L. T, Grieve, R. B., Schantz, P. M. (1986): Serologic pattern of zoonotic pulmonary dirofilariasis. Am. J. Med.; 80: 161–164. DOI: 10.1016/0002-9343(86)90003-3 http://dx.doi.org/10.1016/0002-9343(86)90003-310.1016/0002-9343(86)90003-3
Search in Google Scholar
[20] Gortazar, C., Villafuerte, R., Lucientes, J., Fernandez-De-Luco, D. (1998): Habitat related differences in helminth parasites of red foxes in the Ebro valley. Vet. Parasitol., 15: 75–81. DOI: 10.1016/S0304-4017(98)00192-7 http://dx.doi.org/10.1016/S0304-4017(98)00192-710.1016/S0304-4017(98)00192-7
Search in Google Scholar
[21] Graham, J. M. (1974): Canine filariasis in North Eastern Kansas. J. Parasitol., 60: 322–326. DOI: 10.2307/3278475 http://dx.doi.org/10.2307/327847510.2307/3278475
Search in Google Scholar
[22] Greeve, R. B, Lok, J. B., Glickman, L. T. (1983): Epidemiology of canine heartworm infection. Epidemiol. Rev., 5: 220–246
Search in Google Scholar
[23] Guerrero, J., Ducos De La Hitte, J., Genchi, C., Rojo, F., Gomez-Bautista, M., Carvalho Valera, M., Labar-the, N., Bordini, E., Gonzales, G., Mancebo, O., Patino, F., Uribe, L. F., Samano, R. (1992): Update on the distribution of Dirofilaria immitis in dogs from Southern Europe and Latin America. In: Soll M. D. (Ed) Proc. Heartwom Symp. ′92, American Heartworm Society, Batavia, IL: 31–37
Search in Google Scholar
[24] Guerrero, J., Rodenas, A., Gutierez Galindo, J., Florit, F. (1995): The extension of the prevalence of Dirofilaria immitis in Cataluna, Spain. In: Soll, M. D., Knight, D. H. (Eds) Proc. Heartworm Symp. ′95, American Heartworm Society, Batavia., IL: 73–77
Search in Google Scholar
[25] Haralampidis, S. T. (2003): Parasitic diseases of animals and humans. University Studio Press. Thessaloniki.
Search in Google Scholar
[26] Hoover, J. P., Campbell, G. A., Claypool, P. L., Mullins, S. B. (1996): Comparison of eight diagnostic blood tests for heartworm infection in dogs. Canine Pract. 21: 11–19
Search in Google Scholar
[27] Kitagawa, H., Sasaki, Y., Ishihara, K., Hirano, Y. (1990): Contribution of live heartworms harboring in pulmonary arteries to pulmonary hypertension in dogs with dirofilariasis. Jpn. J. Vet. Sci., 52: 1211–1217
Search in Google Scholar
[28] Marks, C. A., Bloomfield, T. E. (1998): Canine heartworm (Dirofilaria immitis) detected in red foxes (Vulpes vulpes) in urban Melbourne. Vet. Parasitol., 78: 147–154. DOI: 10.1016/S0304-4017(98)00131-9 http://dx.doi.org/10.1016/S0304-4017(98)00131-910.1016/S0304-4017(98)00131-9
Search in Google Scholar
[29] Martin, T. E., Collins, G. H. (1985): Prevalence of Dirofilaria immitis and Dipetalonema reconditum in greyhounds. Aust. Vet. J., 62: 159–163. DOI: 10.1111/j.1751-0813.1985.tb07278.x http://dx.doi.org/10.1111/j.1751-0813.1985.tb07278.x10.1111/j.1751-0813.1985.tb07278.x3842800
Search in Google Scholar
[30] McCall, J. W., Guerrero, J., Genchi, C., Kamer, L. (2004): Recent advances in heartworm disease. Vet. Parasitol., 125: 105–130. DOI: 10.1016/j.vetpar.2004.05.008 http://dx.doi.org/10.1016/j.vetpar.2004.05.00810.1016/j.vetpar.2004.05.008
Search in Google Scholar
[31] Mehlhorn, H. (2001): Encyclopedic Reference of Parasitology. 2nd ed., Disease, Treatment, Therapy. Düsseldorf, Germany, 100–101
Search in Google Scholar
[32] Miterpáková, M., Antolová, D., Hurníková, Z., Dubinský, P. (2008): Dirofilariosis in Slovakia — a new endemic area in Central Europe. Helminthologia, 45,1: 20–23. DOI: 10.2478/s11687-008-0003-6 http://dx.doi.org/10.2478/s11687-008-0003-610.2478/s11687-008-0003-6
Search in Google Scholar
[33] Montoya, J. A., Morales, M., Ferrer, O., Molina, J. M., Corbera, J. A. (1998): The prevalence of Dirofilaria immitis in Gran Canaria, Canary Islands, Spain (1994–1996). Vet. Parasitol., 75: 221–226. DOI: 10.1016/S0304-4017(97)00175-1 http://dx.doi.org/10.1016/S0304-4017(97)00175-110.1016/S0304-4017(97)00175-1
Search in Google Scholar
[34] Muro, A., Genchi, C., Cordero, M., Simon, F. (1999): Human dirofilariasis in the European Union. Parasitol. Today, 15: 386–389. DOI: 10.1016/S0169-4758(99)01496-9 http://dx.doi.org/10.1016/S0169-4758(99)01496-910.1016/S0169-4758(99)01496-9
Search in Google Scholar
[35] Öge, H., Doganay, A., Öge, S., Yildirim, A. (2003): Prevalence and distribution of Dirofilaria immitis in domestic dogs from Ankara and vicinity in Turkey. Dtsch. Tierärztl. Wochenschr., 110: 69–72
Search in Google Scholar
[36] Olteanu, G. (1996): Dirofilariosis in man and animals in Romania. Parasitologia, 38: 360
Search in Google Scholar
[37] Ôncel, T., Vural, G. (2003): Seroprevalence of Dirofilaria immitis in Stray Dogs in Instabul and Izmir. Turk. J. Vet. Anim Sci., 29: 785–789
Search in Google Scholar
[38] Ortega-Mora, L. M., Gomez-Bautista, M., Rojovasquez, F., Rodenas, A., Guerrero, J. (1991): Survey on the prevalence of canine filariasis in Spain. Prev. Vet Med., 11: 63–68. DOI: 10.1016/S0167-5877(05)80045-5 http://dx.doi.org/10.1016/S0167-5877(05)80045-510.1016/S0167-5877(05)80045-5
Search in Google Scholar
[39] Otto, G. F. (1969): Geographical distribution, vectors and life-cycle of Dirofilaria immitis. J. Am. Vet. Med. Assoc., 154: 370–373
Search in Google Scholar
[40] Papazahariadou, M. G., Koutinas, A. F., Rallis, T. S., Haralampidis, S. T. (1994): Prevalence of microfilaemia in episodic weakness and clinically normal dogs belonging to hunting breeds. J. Helmintol., 68: 243–245. DOI: 10.1017/S0022149X00014413 http://dx.doi.org/10.1017/S0022149X0001441310.1017/S0022149X000144137829845
Search in Google Scholar
[41] Poglayen, G., Martini, M., Bomben, L., Roda, R. (1992): An updating of the occurrence of canine heartworm disease in northern Italy. In Proc. VIth EMOP: 114
Search in Google Scholar
[42] Quinn, P. J., Donnelly, W. J. C., Carter, M. E., Markey, B. K. J., Torgerson, P. R., Breathnach, R. M. S. (1997): Microbial and parasitic diseases of the dog and cat. U. S. A., W. B. Saunders Company.
Search in Google Scholar
[43] Rossi, L., Pollono, F., Meneguz, P. G., Gribaudo, L., Balbo, T. (1996): An epidemiological study of canine filarioses in north-west Italy: what has changed in 25 years? Vet. Res. Comm., 20: 308–315. DOI: 10.1007/BF00366536 http://dx.doi.org/10.1007/BF0036653610.1007/BF003665368865572
Search in Google Scholar
[44] Sacks, B. N. (1998): Increasing prevalence of canine heartworm in coyotes from California. J. Wild Dis., 34: 386–389
Search in Google Scholar
[45] Şahal, M., Özlem, M., Tanyel, B., Öcal, N., Sel, T. (1997): The biochemical changes in blood, urine and abdominal fluid in dogs infected by Dirofilaria immilis. Ankara Üniv. Vet. Fak. Derg., 44: 267–276
Search in Google Scholar
[46] Schrey, C. F. (1996): Epidemiologische Fallanalyse der kardiovaskularen Dirofilariose (Herzwurmerkrankung) bei Hunden in Deutschland. Dissertation for PhD in Veterinary Medicine, der Freien Universitat Berlin.
Search in Google Scholar
[47] Schrey, C. F., Trautvetter, E. (1998): Canine and Feline heartworm disease-diagnosis and therapy. Waltham Focus, 8: 23–30
Search in Google Scholar
[48] Segovia, J. M., Torres, J., Miquel, J., Llaneza, L., Feliu, C. (2001): Helminths in the wolf, Canis lupus, from north-western Spain. J. Helmint., 75: 183–192
Search in Google Scholar
[49] Selbey, L. A., Corwin, R. M., Hayes, H. M. (1980): Risk factors associated with canine heartworm infection. J. Am. Vet. Med. Assoc., 176: 33–35
Search in Google Scholar
[50] Shaw, E. S., Day, J. M. (2005): Arthropod-borne infectious diseases of the dog and cat. Manson Publishing Ltd. London. 10.1201/b15174
Search in Google Scholar
[51] Theis, J. H., Stevens, F., Theodoropoulos, G., Ziedins, A. C. (1999): Studies on the prevalence and distribution of filariasis in dogs from Los Angeles County, California (1996–1998). Canine Pract., 24: 8–16.
Search in Google Scholar
[52] Thrasher, J. P., Gould, K. G., Lynch, M. J., Harris, C. C. (1968): Filarial infections of dogs in Atlanta, Georgia. J. Am. Vet. Med. Assoc., 153: 1059–1062
Search in Google Scholar
[53] Weil, G. J., Malane, M. S., Powers, K. G., Blair, L. S. (1985): Monoclonal antibodies to parasite antigens found in the serum of Dirofilaria immitis-infected dogs. J. Immunol., 134: 1185–1191
Search in Google Scholar
[54] Zahler, M., Glaser, B., Gothe, R. (1997): Imported parasites in dogs: Dirofilaria repens and Dipetalonema reconditum. Tierarztl. Prax., 25: 388–392
Search in Google Scholar

An endemic area of Dirofilaria immitis seropositive dogs at the eastern foothills of Mt Olympus, Northern Greece

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