Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic

S. Kumari; M. Lišková

doi:10.2478/s11687-009-0025-8

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Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic

Helminthologia

Volume 46 (2009): Issue 2 (June 2009)

By: S. Kumari and M. Lišková

Open Access

|Jun 2009

Abstract

Identification of the nematode Xiphinema italiae relies mainly on time-consuming morphological and morphometrical studies. A polymerase chain reaction protocol has been used for the reliable and specific identification of X. italiae. Moreover, four independently evolving molecular markers (cox1- cytochrome c oxidase subunit 1; ITS2-second internal transcribed spacer; 18S gene and D2/D3 expansion segments of 28S gene) were amplified and sequenced in both directions.

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Articles in this issue

DOI: https://doi.org/10.2478/s11687-009-0025-8 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

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Language: English

Page range: 131 - 134

Published on: Jun 21, 2009

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Xiphinema italiae,

PCR,

ITS2,

cox1,

18S,

28S

Related subjects:

Life sciences,

Zoology,

Ecology,

Life sciences, other,

Medicine,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2009 S. Kumari, M. Lišková, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 46 (2009): Issue 2 (June 2009)