Molecular genotyping of Anisakis Dujardin, 1845 (Nematoda: Ascaridoidea: Anisakidae) larvae from marine fish of Balinese and Javanese waters, Indonesia

The genetic identification and distribution of Anisakis larvae in Indonesia is described. 110 Auxis rochei rochei and 45 Decapterus russellii were sampled from fish markets in North (Anturan) and South (Kedonganan) Bali. Nematode larvae from A. rochei rochei, Caesio cuning and Epinephelus areolatus from Kedonganan and from Coryphaena hippurus from Pelabuhan Ratu, South Java, were identified using sequence analysis of the internal transcribed spacers (ITS-1, ITS-2) and 5.8S region of rDNA. The larvae belonged to Anisakis typica with an identical sequence to this species from the spinner dolphin (Stenella longirostris) from Brazil, and to 2 further genotypes that differed from that sequence by 0.24–0.47 %. A. typica occurred in the migratory A. rochei rochei and C. hippurus, while Anisakis sp. 1 and 2 were isolated from the same fish species and the non-migratory C. cuning and E. areolatus. The latter genotype is distinguishable by 4 positions in the ITS-1 region (1.1 %), a genetic distance that indicates the presence of an Indonesian A. typica sibling species. The musculature infection in A. rochei rochei was low (2.5 %), indicating no major risk for the fish consumers. The much higher A. typica infection of fish intermediate hosts in the northern Bali coast is suggested to be dependent on the large dolphin population (nematode final hosts) in the waters off Lovina Beach (North Bali).