Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Vadlejch, J.; Langrová, I.; Borovský, M.; Sedmíková, M.; Jankovská, I.; Fechtner, J.; Lytvynets, A.

doi:10.2478/s11687-006-0013-1

Abstract

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio-Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200–220 kDa) decreased in range 32.3–35.4 % and the amount of six polypeptides (20–28 kDa) increased in range 20.0–27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysis were observed between 21 and 34 kDa.

References

[1] Almería, S., LLorente, M. M., Uriarte, J. (1996): Monthly fluctuations of worm burdens and hypobiosis of gastrointestinal nematodes of calves in extensive management systems in the Pyrenees (Spain). Vet. Parasitol., 67: 225–236 http://dx.doi.org/10.1016/S0304-4017(96)01037-010.1016/S0304-4017(96)01037-0
Search in Google Scholar
[2] Armour, J., Jennings, F. W., Urguhart, G. M. (1969): Inhibition of Ostertagia ostertagi at the early fourth stage. The influence of environment on host or parasite. Res. Vet. Sci., 10: 238–244
Search in Google Scholar
[3] Armour, J., Bruce, R. G. (1974): Inhibited development in Ostertagia ostertagi infections — a diapause phenomenon in a nematode. Parasitology, 69: 161–174 10.1017/S0031182000048009
Search in Google Scholar
[4] Connan, R. M. (1971): The Seasonal Incidence of Inhibition of Development in Haemonchus contortus. Res. Vet. Sci., 12: 272–274
Search in Google Scholar
[5] De Cock, H., Knox, D. P., Claerebout, E. DE Graaf, D. C. (1993): Partial characterisation of proteolytic enzymes in different developmental stages of Ostertagia ostertagi. J. Helminthol., 67: 271–278 http://dx.doi.org/10.1017/S0022149X0001326210.1017/S0022149X00013262
Search in Google Scholar
[6] Dopchiz, M. C., Parma, A. E., Fiel, C. A. (2000): Hypobiosis induction alters the protein profile of Ostertagia ostertagi (Nematoda: Trichostrongylidae), Folia Parasitol., 47: 135–140
Search in Google Scholar
[7] Eysker, M. (1993): The role of inhibited development in the epidemiology of Ostertagia infections, Vet. Parasitol., 46: 259–269 http://dx.doi.org/10.1016/0304-4017(93)90063-S10.1016/0304-4017(93)90063-S
Search in Google Scholar
[8] Gamble, H. R., Mansfield, L. S. (1996): Characterisation of excretory — secretory products from larval stages of Haemonchus contortus. Vet. Parasitol., 62: 291–305 http://dx.doi.org/10.1016/0304-4017(95)00871-310.1016/0304-4017(95)00871-3
Search in Google Scholar
[9] Geldhof, P., Claerebout, E., Knox, D. P., Agneessens, J., Vercruysse, J. (2000): Proteinases released in vitro by the parasitic stages of the bovinne abomasal nematode Ostertagia ostertagi. Parasitology, 121: 639–647 http://dx.doi.org/10.1017/S003118200000680610.1017/S0031182000006806
Search in Google Scholar
[10] Hadas, E., Stankiewicz, M. (1997): Proteolytic enzymes of infective larvae and adults of Trichostrongylus colubriformis and Haemonchus contortus. Parasitol. Res., 83: 47–51 http://dx.doi.org/10.1007/s00436005020610.1007/s004360050206
Search in Google Scholar
[11] Healer, J., Ashall, F., Maizels, R. M. (1991): Characterisation of proteolytic enzymes from larval and adult Nippostrongylus brasiliensis. Parasitology, 103: 305–314 http://dx.doi.org/10.1017/S003118200005958810.1017/S0031182000059588
Search in Google Scholar
[12] Jankovská, I., Langrová, I., Vadlejch, J. (2003a): Arrested development of Trichostrongylus colubriformis in experimentally infected rabbits. Effect of acquired resistance, single, multiple doses and size of doses on rise of hypobiosis. Scientia Agriculturae Bohemica, 34: 58–62.
Search in Google Scholar
[13] Jankovská, I., Langrová, I., Fučíková, A., Borovský, M. (2003b): Effect of parasitic nematodes Trichostrongylus colubriformis on haematological parameters of model host (Oryctolagus cuniculus f. domestica). Scientia Agriculturae Bohemica, 34: 94–98
Search in Google Scholar
[14] Kooyman, F. N. J., Eysker, M. (1995): Analysis of proteins related to conditions for arrested development and differentiation in Haemonchus contortus by two — dimmensional gel electrophoresis. Int. J. Parasitol., 25: 561–568 http://dx.doi.org/10.1016/0020-7519(94)00161-G10.1016/0020-7519(94)00161-G
Search in Google Scholar
[15] Langrová, I., Jankovská, I. (2004): Arrested development of Trichostrongylus colubriformis in experimentally infected rabbits. Effect of decreasing photoperiod, low temperature and desiccation. Helminthologia, 41: 85–90
Search in Google Scholar
[16] McKerrow, J. H., Brindley, P., Brown, M., Gam, A., Staunton, C., Neva, A. F. (1990): Strongyloides stercoralis: Identification of a Protease that facilitates Penetration of Skin by the Infective Larva. Exp. Parasitol., 70: 134–143 http://dx.doi.org/10.1016/0014-4894(90)90094-S10.1016/0014-4894(90)90094-S
Search in Google Scholar
[17] Tort, J., Brindley, P. J., Knox, D., Wolfe, K. H., Dalton, J. P. (1999): Proteinases and associated genes of parasitic helminths. Adv. Parasit., 43: 161–166 http://dx.doi.org/10.1016/S0065-308X(08)60243-210.1016/S0065-308X(08)60243-2
Search in Google Scholar

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

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