Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

J. Vadlejch; I. Langrová; M. Borovský; M. Sedmíková; I. Jankovská; J. Fechtner; A. Lytvynets

doi:10.2478/s11687-006-0013-1

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Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Helminthologia

Volume 43 (2006): Issue 2 (June 2006)

By: J. Vadlejch, I. Langrová, M. Borovský, M. Sedmíková, I. Jankovská, J. Fechtner and A. Lytvynets

Open Access

|Jun 2006

Abstract

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio-Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200–220 kDa) decreased in range 32.3–35.4 % and the amount of six polypeptides (20–28 kDa) increased in range 20.0–27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysis were observed between 21 and 34 kDa.

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Articles in this issue

DOI: https://doi.org/10.2478/s11687-006-0013-1 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605

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Language: English

Page range: 64 - 68

Published on: Jun 1, 2006

Published by: Slovak Academy of Sciences, Institute of Parasitology

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Trichostrongylus colubriformis,

hypobiosis,

protein,

enzymes,

SDS-PAGE,

API-ZYM

Related subjects:

Life sciences,

Zoology,

Ecology,

Life sciences, other,

Medicine,

Clinical medicine,

Microbiology, virology and infection epidemiology

© 2006 J. Vadlejch, I. Langrová, M. Borovský, M. Sedmíková, I. Jankovská, J. Fechtner, A. Lytvynets, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 43 (2006): Issue 2 (June 2006)