Polyphenols from Three Invasive Reynoutria Species: Extraction, Characterization, Application
References
- International Union for Conservation of Nature (IUCN). 100 of the World’s Worst Invasive Alien Species [Online]. 2023. [Accessed 28.01.2026]. Available: https://www.iucngisd.org/gisd/100_worst.php
- Commission Implementing Regulation (EU) 2025/1422 of 10 July 2025 establishing a list of invasive alien species of Union concern pursuant to Regulation (EU) No 1143/2014 of the European Parliament and of the Council. Official Journal of the European Union 2025: L 2025/1422.
- Papamatthaiakis N., Laine A., Haapala A., Ikonen R., Kuittinen S., Pappinen A., Mola-Yudego B. New energy crop alternatives for Northern Europe: Yield, chemical and physical properties of giant knotweed (Fallopia sachalinensis var. ‘Igniscum’) and Virginia mallow (Sida hermaphrodita). Fuel 2021:304:121349. https://doi.org/10.1016/j.fuel.2021.121349
- Lavoie C. The impact of invasive knotweed species (Reynoutria spp.) on the environment: review and research perspectives. Biological Invasions 2017:19(8):2319–2337. https://doi.org/10.1007/s10530-017-1444-y
- Purmalis O., Grīnberga L., Kļaviņš L., Kļaviņš M. Quality of lake ecosystems and its role in the spread of invasive species. Environmental and Climate Technologies 2021:25(1):676–687. https://doi.org/10.2478/rtuect-2021-0051
- Bohm V., Castaldi A., Forey E., Chauvat M., Cardinael P., Peulon-Agasse V. Metabolomic fingerprint of Reynoutria japonica (Polygonaceae) using suspect screening with LC-HRMS and untargeted approach with GC-HRMS. Analytical and Bioanalytical Chemistry 2025:418:1203–1217. https://doi.org/10.1007/s00216-025-06273-5
- Mikulic-Petkovsek M., Veberic R., Hudina M., Misic E. HPLC-DAD-MS identification and quantification of phenolic components in Japanese knotweed and American pokeweed extracts and their phytotoxic effect on seed germination. Plants 2022:11(22):3053. https://doi.org/10.3390/plants11223053
- Liu S., Zhang R., Zhang X., Zhu S., Liu S., Yang J., Hu H. The invasive species Reynoutria japonica Houtt. as a promising natural agent for cardiovascular and digestive system illness. Frontiers in Pharmacology 2022:13:863707. https://doi.org/10.3389/fphar.2022.863707
- Zhang Z. L., Li Y. Z., Wu G. Q., Zhang D. D., Deng C., Wang Z. M., Wang W. A comprehensive review of traditional uses, phytochemistry and pharmacology of Reynoutria genus. Journal of Pharmacy and Pharmacology 2022:74(12):1718–1742. https://doi.org/10.1093/jpp/rgac068
- Wang Z. J., Trill J., Tan L. L., Chang W. J., Zhang Y., Willcox M., Hu X. Y. Reynoutria japonica Houtt for acute respiratory tract infections in adults and children: a systematic review. Frontiers in Pharmacology 2022:13:787032. https://doi.org/10.3389/fphar.2022.787032
- European Pharmacopoeia. 9th ed. Strasbourg: Council of Europe. 2017:1481–1483.
- Jiang L., Zhang J., Zhu B., Bao X., Tian J., Li Y., Long F. The aqueous extract of Reynoutria japonica ameliorates damp-heat ulcerative colitis in mice by modulating gut microbiota and metabolism. Journal of Ethnopharmacology 2025:338:119042. https://doi.org/10.1016/j.jep.2024.119042
- Borska E., Kviesis J., Ansone-Bertina L., Klavins M. Invasive Plant Biomass as a Source of Lipids for Bioeconomy. Environmental and Climate Technologies 2025:29(1):233–242. https://doi.org/10.2478/rtuect-2025-0016
- Razgonova M. P., Cherevach E. I., Kirilenko N. S., Demidova E. N., Golokhvast K. S. Determining the polyphenol complex in Reynoutria japonica Houtt. by tandem mass spectrometry method. Russian Journal of Plant Physiology 2024:71(3):99. https://doi.org/10.1134/S1021443724606049
- Lee H., Kim Y. J., Park K., Heo H., Lee J., Kim J. Y., Cho J. S. Evaluation of biological activities depending on different part and harvesting time of Reynoutria sachalinensis. Horticulture, Environment, and Biotechnology 2025:66:1689–1701. https://doi.org/10.1007/s13580-025-00764-1
- Fan L., Wang Y., Xie P., Zhang L., Li Y., Zhou J. Copigmentation effects of phenolics on color enhancement and stability of blackberry wine residue anthocyanins: Chromaticity, kinetics and structural simulation. Food Chemistry 2019:275:299–308. https://doi.org/10.1016/j.foodchem.2018.09.103
- Sun Y., Huang F., Chen Y., Ning N., Hao G., Bi X. The effect of high-pressure processing on the copigmentation and storage stability of polyphenols with anthocyanin monomers. Foods 2024:13(23):3756. https://doi.org/10.3390/foods13233756
- Tan C., Dadmohammadi Y., Lee M. C., Abbaspourrad A. Combination of copigmentation and encapsulation strategies for the synergistic stabilization of anthocyanins. Comprehensive Reviews in Food Science and Food Safety 2021:20(4):3164–3191. https://doi.org/10.1111/1541-4337.12772
- Wang J., Zhao Y., Sun B., Yutong Y., Wang S., Feng Z., Li J. The structure of anthocyanins and the copigmentation by common micromolecular copigments: A review. Food Research International 2024:176:113837. https://doi.org/10.1016/j.foodres.2023.113837
- Yücetepe M., Özaslan Z. T., Karakuş M. Ş., Akalan M., Karaaslan A., Karaaslan M., Başyiğit B. Unveiling the multifaceted world of anthocyanins: Biosynthesis pathway, natural sources, extraction methods, copigmentation, encapsulation techniques, and future food applications. Food Research International 2024:187:114437. https://doi.org/10.1016/j.foodres.2024.114437
- Azman E. M., Yusof N., Chatzifragkou A., Charalampopoulos D. Stability enhancement of anthocyanins from blackcurrant (Ribes nigrum L.) pomace through intermolecular copigmentation. Molecules 2022:27(17):5489. https://doi.org/10.3390/molecules27175489
- Zihare L., Muizniece I., Blumberga D. New Vision on Invasive Alien Plant Management System. Environmental and Climate Technologies 2019:23(2):166–186. https://doi.org/10.2478/rtuect-2019-0062
- Attard E. A rapid microtiter plate Folin–Ciocalteu method for the assessment of polyphenols. Open Life Sciences 2013:8(1):48–53. https://doi.org/10.2478/s11535-012-0107-3
- Purmalis O., Klavins L., Niedrite E., Mezulis M., Klavins M. Invasive plants as a source of polyphenols with high radical scavenging activity. Plants 2025:14(3):467. https://doi.org/10.3390/plants14030467
- Fukumoto L. R., Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of Agricultural and Food Chemistry 2000:48(8):3597–3604. https://doi.org/10.1021/jf000220w
- Lachowicz S., Oszmiański J. Profile of bioactive compounds in the morphological parts of wild Fallopia japonica (Houtt) and Fallopia sachalinensis (F. Schmidt) and their antioxidative activity. Molecules 2019:24(7):1436. https://doi.org/10.3390/molecules24071436
- Dini I., Grumetto L. Recent advances in natural polyphenol research. Molecules 2022:27(24):8777. https://doi.org/10.3390/molecules27248777
- Chaves J. O., De Souza M. C., Da Silva L. C., Lachos-Perez D., Torres-Mayanga P. C., Machado A. P. D. F., Forster- Carneiro T., Vázquez-Espinosa M., González-de-Peredo A. V., Barbero G. F., Rostagno M. A. Extraction of flavonoids from natural sources using modern techniques. Frontiers in Chemistry 2020:8:507887. https://doi.org/10.3389/fchem.2020.507887
- Ermi Hikmawanti N. P., Fatmawati S., Asri A. W. The effect of ethanol concentrations as the extraction solvent on antioxidant activity of Katuk (Sauropus androgynus (L.) Merr.) leaves extracts. IOP Conference Series: Earth and Environmental Science 2021:755(1):012060. https://doi.org/10.1088/1755-1315/755/1/012060
- Ngo T. V., Scarlett C. J., Bowyer M. C., Ngo P. D., Vuong Q. V. Impact of different extraction solvents on bioactive compounds and antioxidant capacity from the root of Salacia chinensis L. Journal of Food Quality 2017:2017(1):9305047. https://doi.org/10.1155/2017/9305047
- Zuo A. R., Dong H. H., Yu Y. Y., Shu Q. L., Zheng L. X., Yu X. Y., Cao S. W. The antityrosinase and antioxidant activities of flavonoids dominated by the number and location of phenolic hydroxyl groups. Chinese Medicine 2018:13(1):51. https://doi.org/10.1186/s13020-018-0206-9
- Muceniece R., Revina B. L., Kviesis J., Jansons A., Kopiks K., Jekabsons K., Saleniece K., Namniece J., Grigale-Sorocina Z., Jansone B. Nettle leaf water extracts for hepatoprotection: Insights into bioactivity and mitochondrial function. Plants 2025:14:992. https://doi.org/10.3390/plants14070992
- Suprun A. R., Kiselev K. V., Aleynova O. A., Manyakhin A. Y., Ananev A. A. Analysis of Phenolic Compounds of Reynoutria sachalinensis and Reynoutria japonica Growing in the Russian Far East. Plants 2024:13(23):3330. https://doi.org/10.3390/plants13233330
- Nawrot-Hadzik I., Granica S., Domaradzki K., Pecio Ł., Matkowski A. Isolation and determination of phenolic glycosides and anthraquinones from rhizomes of various Reynoutria species. Planta Medica 2018:84(15):1118–1126. https://doi.org/10.1055/a-0605-3857
- Bensa M., Glavnik V., Vovk I. Flavan-3-ols and proanthocyanidins in Japanese, Bohemian and Giant knotweed. Plants 2021:10(2):402. https://doi.org/10.3390/plants10020402
- Do D. T., Harbourne N., Ellis A. Anthocyanins: Anthocyanidins, berries, colorants, and copigmentation. Handbook of Food Bioactive Ingredients: Properties and Applications. Cham: Springer International Publishing, 2023:341–364. https://doi.org/10.1007/978-3-031-28109-9_9
- Wang X., Cheng J., Zhu Y., Li T., Wang Y., Gao X. Intermolecular copigmentation of anthocyanins with phenolic compounds improves color stability in the model and real blueberry fermented beverage. Food Research International 2024:190:114632. https://doi.org/10.1016/j.foodres.2024.114632
- Idir S., Achat S., Cruz L., Dangles O. Anthocyanin-rich extracts: Susceptibility to color loss by hydration and thermal degradation, influence of metal ions and endogenous copigments. Food Chemistry 2025:481:144004. https://doi.org/10.1016/j.foodchem.2025.144004
- Perdani A. W., Setiowati A. D., Purwono B., Supriyadi S. Color enhancement of rambutan peel anthocyanins extracts using co-pigmentation with gallic acid for pH-sensitives dye. Trends in Sciences 2025:22(4):9518. https://doi.org/10.48048/tis.2025.9518
- Chen Y., Belwal T., Xu Y., Ma Q., Li D., Li L., Luo Z. Updated insights into anthocyanin stability behavior from bases to cases: Why and why not anthocyanins lose during food processing. Critical Reviews in Food Science and Nutrition 2023:63(27):8639–8671. https://doi.org/10.1080/10408398.2022.2063250
DOI: https://doi.org/10.2478/rtuect-2026-0037 | Journal eISSN: 2255-8837 (formerly 2255-8845) | Journal ISSN: 1691-5208
Language: English
Page range: 542 - 557
Submitted on: Apr 7, 2026
Accepted on: May 29, 2026
Published online: Oct 28, 2026
Published in print: Jan 1, 2026
Published by: Riga Technical University
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open
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© 2026 Linda Ansone-Bertina, Linards Klavins, Jorens Kviesis, Evelina Niedrite, Alise Zommere, Maris Klavins, published by Riga Technical University
This work is licensed under the Creative Commons Attribution 4.0 License.