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Comparative analysis of the impact of a high-fat, high-carbohydrate diet on two generations of naïve mice Cover

Comparative analysis of the impact of a high-fat, high-carbohydrate diet on two generations of naïve mice

Open Access
|Feb 2026

References

  1. Lindasy M J, Vandevijvere S, Pan A, McGowan CJ, Wallace C, Imamura F, et al. The obesity transition: stages of the global epidemic. Lancet Diabetes Endocrinol. 2019;7(3):231-40. DOI: 10.1016/S2213-8587(19)30026-9
  2. Thomsen MN, Skytte MJ, Samkani A, Carl MH, Weber P, Astrup A, et al. Dietary carbohydrate restriction augments weight loss-induced improvements in glycaemic control and liver fat in individuals with type 2 Diabetes: a randomised controlled trial. Diabetologia. 2022;65(3):506-17. DOI: 10.1007/s00125-021-05628-8
  3. Castro-Perez JM, Roddy TP, Shah V, McLaren DG, Wang SP, Jensen K, et al. Identifying static and kinetic lipid phenotypes by high resolution UPLC-MS: unraveling diet-induced changes in lipid homeostasis by coupling metabolomics and Fluxomics. J Proteome Res.2011;10:4281-90. DOI: 10.1021/pr200480g
  4. Moore R, Anturaniemi J, Velagapudi V, Nandania J, Barrouin-Melo SM, and Hielm-Björkman A. Targeted metabolomics with Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) highlights metabolic differences in healthy and atopic Staffordshire bull terriers fed two different diets, a pilot study. Front Vet Sci. 2020; 7:554296. DOI: 10.3389/fvets.2020.554296
  5. Longo M, Zatterale, F, Naderi J, Parrillo L, Formisano P, Raciti GA, et al. Adipose Tissue Dysfunction as Determinant of Obesity-Associated Metabolic Complications. Int J Mol Sci. 2019;20:2358. DOI: 10.3390/ijms20092358
  6. Mozaffarian D. Dietary and Policy Priorities for Cardiovascular Disease, Diabetes, and Obesity: A Comprehensive Review. Circulation 2016;133:187-225. DOI: 10.1161/CIRCULATIONAHA.115.018585
  7. Whytock KL, Shepherd SO, Cocks M, Wagenmakers A, Strauss JA. Young, healthy males and females present cardiometabolic protection against the detrimental effects of a 7-day high-fat high-calorie diet. Eur J Nutr. 2021;60:1605-17. DOI: 10.1007/s00394-020-02357-3
  8. Bisschop P, Bandsma R, Stellaard F, ter Harmsel A, Meijer A, Sauerwein H, et al. Low-fat, high-carbohydrate and high-fat, low-carbohydrate diets decrease primary bile acid synthesis in humans. Am. J. Clin. Nutr. 2004;79:570-576. DOI: 10.1093/ajcn/79.4.570
  9. Misra B, Puppala S, Comuzzie A, Mahaney M, VandeBerg J, Olivier M, et al. Analysis of serum changes in response to a high fat high cholesterol diet challenge reveals metabolic biomarkers of atherosclerosis. PLoS ONE. 2019;14: e0214487. DOI: 10.1371/journal.pone.0214487
  10. Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, et al. Fast food diet mouse: Novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest. Liver Physiol. 2011;301:G825-34. DOI: 10.1152/ajpgi.00145.2011
  11. Tomasello G, Mazzola M, Leone A, Sinagra E, Zummo G, Farina F, et al. Nutrition, oxidative stress and intestinal dysbiosis: Influence of diet on gut microbiota in inflammatory bowel diseases. Biomed. Pap. Med. Fac. Univ. Palacky. Olomouc Czech Repub. 2016;160:461-6. DOI: 10.5507/bp.2016.052
  12. Chen Q, Yan M, Cao Z, Li X, Zhang Y, Shi J, et al. Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder. Science. 2016;351:397-400. DOI: 10.1126/science.aad7977
  13. Agarwal A, Majzoub A. Free Radicals in Andrology. Springer; 2017. DOI: 10.1007/978-3-319-41749-3_1
  14. Kubandova J, Fabiana D, Burkusa J, Čikosa S, Czikkova S, Mozes S, et al. Two-Generation Diet-Induced Obesity Model Producing Mice With Increased Amount of Body Fat in Early Adulthood. Physiol. Res. 2014;63:103-13. DOI: 10.33549/physiolres.932547
  15. Kurien B, Everds N, Scofield RH. Experimental animal urine collection: a review. Laboratory Animals. 2004;38:333-61. DOI: 10.1258/0023677041958945
  16. Ayman Alhazmi. Commiphora gileadensis ameliorate Infertility and Erectile Dysfunction in Diabetic Male Mice. Open Med (Wars). 2025;20(1):20251166. DOI: 10.1515/med-2025-1166
  17. Wick MR. The hematoxylin and eosin stain in anatomic pathology-An often-neglected focus of quality assurance in the laboratory. Seminars in Diagnostic Pathology. 2019;36(5):303-11. DOI: 10.1053/j.semdp.2019.06.003
  18. Bastías-Pérez M, Serra D, Herrero L. Dietary Options for Rodents in the Study of Obesity. Nutrients. 2020;12(11):3234. DOI: 10.3390/nu12113234
  19. Picó C, Palou M, Pomar CA, Rodríguez AM, Palou A, et al. Leptin as a key regulator of the adipose organ. Rev Endocr Metab Disord. 2021;23(1):13-30. DOI: 10.1007/s11154-021-09687-5
  20. Baldelli S, Aiello G, Mansilla Di Martino E, Campaci D, Fares Muthanna MS, Lombardo M, et al. The Role of Adipose Tissue and Nutrition in the Regulation of Adiponectin. Nutrients. 2024;16(15):2436. DOI: 10.3390/nu16152436
  21. Franco JG, Fernandes TP, Rocha CP, Calviño C, Pazos-Moura CC, Lisboa PC, et al. Maternal high-fat diet induces obesity and adrenal and thyroid dysfunction in male rat offspring at weaning. J Physiol. 2012;590(21):5503-18. DOI: 10.1113/jphysiol.2012.240655
  22. Gregorio BM, Souza-Mello V, Carvalho JJ, Mandarim-de-Lacerda CA, Aguila MB, et al. Maternal high-fat intake predisposes nonalcoholic fatty liver disease in C57BL/6 offspring. Am J Obstet Gynecol. 2010;203:e491-e498. DOI: 10.1016/j.ajog.2010.06.042
  23. Jesús Tejero, Sruti Shiva, Mark T. Gladwin. Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation. 2019;99(1):311-79. DOI: 10.1152/physrev.00036.2017
  24. Rooban S, Arul Senghora KA, Vinodhini VM, Kumar JS. Adropin: A crucial regulator of cardiovascular health and metabolic balance. Metabol Open. 2024 Jun 27;23:100299. DOI: 10.1016/j. metop.2024.100299
  25. Kostov K. The Causal Relationship between Endothelin-1 and Hypertension: Focusing on Endothelial Dysfunction, Arterial Stiffness, Vascular Remodeling, and Blood Pressure Regulation. Life. 2021;11(9):986. DOI: 10.3390/life11090986
  26. Meza-Alvarado JC, Page RA, Mallard B, Bromhead C, Palmer BR. VEGF-A related SNPs: a cardiovascular context. Front Cardiovasc Med. 2023;10:1190513. DOI: 10.3389/fcvm.2023.1190513
  27. Caturano A, D’Angelo A, Mormone A, Russo V, Mollica MP, Salvatore T, et al. Oxidative Stress in Type 2 Diabetes: Impacts from Pathogenesis to Lifestyle Modifications. Curr Issues Mol Biol. 2023 Aug 12;45(8):6651-66. DOI: 10.3390/cimb45080420
  28. Zhang Q, Xiao X, Zheng J, Li M, Yu M, Ping F, et al. A Maternal High-Fat Diet Induces DNA Methylation Changes That Contribute to Glucose Intolerance in Offspring. Front Endocrinol (Lausanne). 2019;10:871. DOI: 10.3389/fendo.2019.00871
DOI: https://doi.org/10.2478/rrlm-2026-0002 | Journal eISSN: 2284-5623 | Journal ISSN: 1841-6624
Language: English
Page range: 47 - 54
Submitted on: Jul 7, 2025
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Accepted on: Dec 2, 2025
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Published on: Feb 5, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 Ayman Alhazmi, Mamdouh Allahyani, published by Romanian Association of Laboratory Medicine
This work is licensed under the Creative Commons Attribution 4.0 License.