Inhibition of Streptococcus pneumoniae adenylate kinase by some 5-arylidene-thiazolidin-4-on-2-thione derivates

Miliaela Ileana Ionescu; Adriana Z. Costache; Ovidiu Oniga; Horia L. Banciu; Iulia Lupan

doi:10.2478/rrlm-2013-0022

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Inhibition of Streptococcus pneumoniae adenylate kinase by some 5-arylidene-thiazolidin-4-on-2-thione derivates

Revista Romana de Medicina de Laborator

Volume 21 (2013): Issue 1 (March 2013)

By: Miliaela Ileana Ionescu, Adriana Z. Costache, Ovidiu Oniga, Horia L. Banciu and Iulia Lupan

Open Access

|Aug 2013

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Abstract

Adenylat kinase is an ubiquitous enzyme found in prokaryotes and eukaryotes. In the present study we examined the inhibition of Streptococcus pneumoniae adenylate kinase (AKSP) by six 5-arylidene-thiazolidin-4- on-2-thione derivates using 2, 4-dinitrophenylhydrazine colorimetric assay. Inhibition of AKSP activity with synthetic compounds was performed against recombinant enzyme over-expressed in E. coli. The compound C₁₀H₆BrNOS₂ with the bromine atom in -ortho position has shown the most efficient inhibitory activity; I50 value (the inhibitor concentration that leads to 50% activity inhibition) was 0.067mM.

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Articles in this issue

DOI: https://doi.org/10.2478/rrlm-2013-0022 | Journal eISSN: 2284-5623 | Journal ISSN: 1841-6624 (formerly 2284-5623)

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Language: English, Romanian

Page range: 93 - 99

Published online: Aug 8, 2013

Published in print: Mar 1, 2013

Published by: Romanian Association of Laboratory Medicine

In partnership with: Paradigm Publishing Services

Keywords:

adenylate kinase,

5-arylidene-thiazolidin-4-on-2-thione derivates,

inhibitory activity

Related subjects:

Life sciences,

Molecular biology

© 2013 Miliaela Ileana Ionescu, Adriana Z. Costache, Ovidiu Oniga, Horia L. Banciu, Iulia Lupan, published by Romanian Association of Laboratory Medicine
This work is licensed under the Creative Commons License.

Volume 21 (2013): Issue 1 (March 2013)