FIGURE 1.
Diagram of the BCL2/JH t(14;18) translocation breakpoints. Relative positions of major breakpoint region (MBR), 3′MBR subcluster, 5′mcr subcluster and minor cluster region (mcr) are shown according to the report of van Dongen JJM et al.16
TABLE 1.
Sequences of primers used for detection of the t(14;18)(q32;q21) translocation. Relative positions of primers are indicated downstream of the first nucleotide of corresponding reference sequence
| t(14;18) MBR primers | |||
|---|---|---|---|
| primer name | NCBI accession no. | position | primer sequence |
| MBR1 | AY220759.1 | (+193443) | 5′-GACCAGCAGATTCAAATCTATGG-3′ |
| MBR2 | AY220759.1 | (+192940) | 5′-ACTCTGTGGCATTATTGCATTATAT-3′ |
| t(14;18) 3′MBR primers | |||
| primer name | NCBI accession no. | position | primer sequence |
| 3′MBR1 | AH010747.2 | (+717) | 5′-GCACCTGCTGGATACAACACTG-3′ |
| 3′MBR2 | AH010747.2 | (+1530) | 5′-GGTGACAGAGCAAAACATGAACA-3′ |
| 3′MBR3 | AH010747.2 | (+1787) | 5′-GTAATGACTGGGGAGCAAATCTT-3′ |
| 3′MBR4 | AH010747.2 | (+2718) | 5′-ACTGGTTGGCGTGGTTTAGAGA-3′ |
| t(14;18) mcr primers | |||
| primer name | NCBI accession no. | position | primer sequence |
| mcr1 | AF275873.1 | (+1961) | 5′-TAGAGCAAGCGCCCAATAAATA-3′ |
| mcr2 | AF275873.1 | (+2407) | 5′-TGAATGCCATCTCAAATCCAA-3′ |
| 5′mcr | AH010747.2 | (+15849) | 5′-CCTTCTGAAAGAAACGAAAGCA-3′ |
| Consensus JH primer | |||
| primer name | NCBI accession no. | position | primer sequence |
| JH | OL807663.1 | (+239) | 3′-CCAGTGGCAGAGGAGTCCATTC-5′ |
[i] AF275873.1 = homo sapiens BCL2 gene, exon 3 and breakpoint region; AH010747.2 = homo sapiens genomic sequence downstream of BCL2; AY220759.1 = homo sapiens B-cell CLL/lymphoma 2 (BCL2) gene, complete coding sequence; MBR = major breakpoint region; mcr = minor cluster region; NCBI = National Center for Biotechnology Information; OL807663.1 = homo sapiens clone J6 immunoglobulin heavy chain variable region gene, partial coding sequence
TABLE 2.
Comparison of clinical features at diagnosis between the breakpoint-site groups (MBR, 3′MBR, mcr) using Fisher's exact test
| MBR (N = 63) | 3′MBR (N = 4) | mcr (N = 17) | p1 | p2 | |
|---|---|---|---|---|---|
| Male sex | 24 (38%) | 1 (25%) | 4 (24%) | 0.571 | 0.391 |
| Grade* 3 | 11 (20%) | 2 (50%) | 4 (25%) | 0.303 | 0.729 |
| B-symptoms | 23 (37%) | 2 (50%) | 8 (47%) | 0.641 | 0.576 |
| Bulky disease** | 17 (27%) | 1 (25%) | 2 (12%) | 0.497 | 0.335 |
MBR = major breakpoint region; mcr = minor cluster region; p1 = significance comparing all 3 groups; p2 = significance comparing the MBR and mcr groups only;
TABLE 3.
Comparison of clinical features at diagnosis between the breakpoint-site groups (major breakpoint region [MBR], 3′MBR, mcr)
| MBR (N = 63) | 3′MBR (N = 4) | mcr (N = 17) | p1 | p2 | |
|---|---|---|---|---|---|
| Median (mean) stage | 4 (3.70) | 4 (3.75) | 4 (3.94) | 0.361 | 0.023 |
| Median (mean) FLIPI | 2 (2.51) | 3 (2.75) | 3 (3.00) | 0.226 | 0.094 |
| Median (mean) age | 61 (60.25) | 62 (63.25) | 64 (63.71) | 0.423 | 0.218 |
[i] p1 = significance comparing all 3 groups using 1 way Analysis of Variance (ANOVA) (df = 2); p2 = significance comparing the major breakpoint region (MBR) and minor cluster region (mcr) groups using Independent-Samples T-test
FIGURE 2.
Comparison of progression-free survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.157. Log Rank (Mantel-Cox) significance comparing the MBR and mcr group: 0.235.
MBR = major breakpoint region; mcr = minor cluster region
FIGURE 3.
Comparison of overall survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.426. Log Rank (Mantel-Cox) significance comparing the MBR and mcr group: 0.351.
MBR = major breakpoint region; mcr = minor cluster region
FIGURE 4.
Comparison of lymphoma-specific overall survival between the 3′MBR (blue), MBR (red) and mcr (green) groups. Censored cases are marked as vertical lines on their respective curves. Log Rank (Mantel-Cox) significance: 0.409. Log Rank (Mantel-Cox) significance comparing the MBR and mcr groups: 0.301.
MBR = major breakpoint region, mcr = minor cluster region