Have a personal or library account? Click to login
Expression of DNA-damage response and repair genes after exposure to DNA-damaging agents in isogenic head and neck cells with altered radiosensitivity Cover

Expression of DNA-damage response and repair genes after exposure to DNA-damaging agents in isogenic head and neck cells with altered radiosensitivity

Radiology and Oncology
Volume 56 (2022): Issue 2 (June 2022)
By:
Open Access
|Apr 2022

Figures & Tables

Figure 1

Overall expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001
Overall expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001

Figure 2

2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.
2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.

Figure 3

Deregulated expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05; ** p < 0.01; *** p < 0.001
Deregulated expression of DNA-damage signaling genes in (parental, HPV-negative) FaDu cells and (HPV-positive) 2A3 cells after exposure to cisplatin (CDDP), radiation (IR) or their combination (CDDP+IR). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05; ** p < 0.01; *** p < 0.001

Figure 4

Overall gene expression in (parental, HPV-negative) FaDu cells and (radioresistant) FaDu-RR cells in response to cisplatin (CDDP) and bleomycin (BLEO). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001
Overall gene expression in (parental, HPV-negative) FaDu cells and (radioresistant) FaDu-RR cells in response to cisplatin (CDDP) and bleomycin (BLEO). Symbols are mean gene expression for each of the 84 DNA-damage signaling genes included on the Human DNA Damage Signaling Pathway RT2 ProfilerTM PCR Array from 3 independent experiments. The lines indicate the median gene expression of all tested genes.* p < 0.05; *** p < 0.001; **** p < 0.0001

Figure 5

2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.
2-D clustergram analysis of the expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Clusters observed at ¼ dendrogram branch height are shown as blocks of different color.

Figure 6

Deregulated expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05
Deregulated expression of DNA-damage signaling genes in (parental) FaDu cells and (radioresistant) FaDu-RR cells after exposure to cisplatin (CDDP) or bleomycin (BLEO). Only significantly deregulated genes are shown. Symbols are the mean and SEM from three independent experiments.* p < 0.05
DOI: https://doi.org/10.2478/raon-2022-0014 | Journal eISSN: 1581-3207 | Journal ISSN: 1318-2099
Journal RSS Feed
Language: English
Page range: 173 - 184
Submitted on: Feb 25, 2022
Accepted on: Mar 16, 2022
Published on: Apr 7, 2022
Published by: Association of Radiology and Oncology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
head and neck cancer,
squamous cell carcinoma,
radioresistance,
irradiation,
DNA-damaging agents,
gene expression profiles
Related subjects:
Medicine,
Clinical medicine,
Internal medicine,
Haematology, oncology,
Radiology

© 2022 Vesna Todorovic, Blaz Groselj, Maja Cemazar, Ajda Prevc, Martina Niksic Zakelj, Primoz Strojan, Gregor Sersa, published by Association of Radiology and Oncology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 56 (2022): Issue 2 (June 2022)Next article