Clinical outcome of in vitro fertilization / intracytoplasmic sperm injection (IVF/ICSI) cycles according to the type of semen used_ Statistically significant difference is marked with an asterisk (p value < 0_05)
| Cryopreserved semen | Fresh semen | p values | |
|---|---|---|---|
| Number of all ETs | 109 | 67 | 0.45 |
| Number of ETs on day 3 (% per all ETs) | 27 (24.8%) | 15 (22.4%) | 0.72 |
| Mean number of transferred embryos (± SD) | 1.6 ± 0.5 | 1.6 ± 0.5 | 0.81 |
| Number of pregnancies (% per ET) | 39 (35.8%) | 18 (26.9%) | 0.22 |
| Number of pregnancies (% per oocyte aspiration) | 39 (30.0%) | 18 (21.4%) | 0.12 |
| Live births (% per ET) | 29 (26.6%) | 15 (22.4%) | 0.53 |
| Live births (% per aspiration) | 29 (22.3%) | 15 (17.9%) | 0.43 |
| Miscarriages | 10 (25.6%) | 2 (+(11.11 x EU) %) | 0.21 |
| Gestational age (all births) | 38.4 ± 2.6 | 36.0 ± 5.1 | 0.14 |
| Gestational age for singletons | 38.5 ± 2.7 | 36.6 ± 4.9 | 0.18 |
| Birth weight of singletons (g) | 3300 ± 589 | 2959 ± 672 | 0.12 |
| Twins | 2 (6.9%) | 1 (6.7%) | 1 |
| Cumulative number and rate of couples with at least one pregnancy | 43 (60.6%) | 20 (37.7%) | 0.012* |
| Cumulative number and rate of couples with at least one live birth | (+4 ongoing 32 (45.1pregnancies) %) | 18 (34.0%) | 0.21 |
The outcome of the in vitro fertilization / intracytoplasmic sperm injection (IVF/ICSI) cycles in terms of oocytes and embryos according to the type of semen used_ Statistically significant differences are marked with an asterisk (p value < 0_05)_
| Cryopreserved semen | Fresh semen | p value | |
|---|---|---|---|
| Rate of normally fertilized oocytes (per COC, IVF and ICSI together) | 46.3% | 50.4% | 0.06 |
| Rate of normally fertilized oocytes (per MII oocytes number) | 55.6% | 59.3% | 0.16 |
| Number and rate of immature oocytes (%) | 225 (17.7%) | 138 (16.6%) | 0.54 |
| Number and rate of degenerated oocytes (% per COC, IVF and ICSI together) | 171 (13.4%) | 91 (11.0%) | 0.10 |
| Number and rate of degenerated oocytes (% per MII oocytes number) | 166 (17.0%) | 88 (15.5%) | 0.43 |
| Number and rate of polyploidies (% per all COC retrieved) | 13 (1.0%) | 13 (1.6%) | 0.27 |
| Number and proportion of embryos (% per normally fertilized oocytes) | 560 (95.1%) | 403 (96.4%) | 0.31 |
| Mean number of embryos per cycle (mean number ± SD) | 4.3 ± 3.4 | 4.8 ± 3.8 | 0.33 |
| Number of embryos cultured until day 5/6 | 518 (92.5%) | 382 (94.8%) | 0.16 |
| Number and rate of blastocysts (% per embryos cultured until day 5/6) | 177 (34.2%) | 112 (29.3%) | 0.12 |
| Number and rate of embryo utilization (transferred plus frozen embryos) | 274 (48.9%) | 161 (40.0%) | 0.006* |
| Number of cycles with at least one blastocyst | 74 (56.9%) | 47 (56.0%) | 0.89 |
| Number and rate of cryopreserved embryos (% of all embryos) | 97 (17.3%) | 51 (12.7%) | 0.048* |
| Number and proportion of cycles with embryo cryopreservation | 39 (30.0%) | 16 (19.0%) | 0.07 |
| Number and proportion of cycles with freezing/without ET | 4 (3.1%) | 2 (2.4%) | 1 |
| Number and proportion of cycles without freezing/without ET | 17 (13.1%) | 15 (17.9%) | 0.34 |
Types of cancer in male patients, and the number of cases for each cancer type in the cryopreserved and fresh semen groups
| Cryopreserved semen (N) | Fresh semen(N) | |
|---|---|---|
| Testicular cancer | 39 | 36 |
| Hodgkin lymphoma | 16 | 7 |
| Non-Hodgkin lymphoma | 0 | 1 |
| Burkitt lymphoma | 1 | 0 |
| Leukemia | 7 | 5 |
| Plasmacytoma | 3 | 1 |
| Rectal cancer | 1 | 2 |
| Brain tumor | 1 | 0 |
| Malignant melanoma | 1 | 0 |
| Prostate cancer | 1 | 0 |
| Epipharynx tumor | 0 | 1 |
| Together | 70 | 53 |
The baseline characteristics of patients and in vitro fertilization / intracytoplasmic sperm injection (IVF/ICSI) cycles_ Statistically significant differences are marked with an asterisk (p value < 0_05)
| Cryopreserved semen | Fresh semen | p values | |
|---|---|---|---|
| Causes of female infertility | |||
| Tubal factor | 2 (1.5%) | 1 (1.2%) | 1 |
| Endometriosis | 2 (1.5%) | 2 (2.4%) | 1 |
| Endocrine disorders | 16 (12.3%) | 2 (2.4%) | 0.011* |
| Uterine factor | 9 (6.9%) | 11 (13.1%) | 0.13 |
| Cervical factor | 0 | 5 (6.0%) | 0.009* |
| Multiple causes of female infertility | 15 (11.5%) | 20 (23.8%) | 0.018* |
| No cause of female infertility | 86 (66.2%) | 43 (51.2%) | 0.029* |
| Ovarian stimulation protocols | |||
| Long agonist protocol | 74 (56.9%) | 53 (63.1%) | 0.37 |
| Short antagonist protocol | 55 (42.3%) | 30 (35.7%) | 0.33 |
| Others | 1 (0.8%) | 1 (1.2%) | 1 |
| Number of cycles | 130 | 84 | |
| Number of couples | 71 | 53 | |
| Female mean age (± SD) | 31.7±4.8 | 32.3±4.3 | 0.31 |
| Number and rate of ICSI cycles | 122 (93.8%) | 40 (84.5%) | 0.025* |
| Oocyte number (mean number per cycle) Number of MII oocytes (ICSI cycles only) | 1273 (9.8±5.2) 974 | 829 (9.9±6.7) 568 | 0.93 |