Comparison of NF-κB, Heat Shock Proteins, and Galectin-10 Expression in Cleft-Affected Hard Tissues Between Different Facial Sides
By: Māris Puzāks, Ilze Akota and Māra Pilmane
References
- Ackerman, S. J., Weil, G. J., Gleich, G. J. (1982). Formation of Char-cot-Leyden crystals by human basophils. J. Exp. Med., 155 (6), 1597–1609.
- Berger, E., Rath, E., Yuan, D., Waldschmitt, N., Khaloian, S., Allgäuer, M., et al. (2016). Mitochondrial function controls intestinal epithelial stemness and proliferation. Nat. Commun., 7, 13171.
- Biancalana, M., Natan, E., Lenardo, M. J., Fersht, A. R. (2021). NF-κB Rel subunit exchange on a physiological timescale. Protein Sci., 30 (9), 1818–1832. DOI: 10.1002/pro.4134. PMID: 34089216; PMCID: PMC8376415.
- Boyce, B. F., Xiu, Y., Li, J., Xing, L., Yao, Z. (2015). NF-κB-mediated regulation of osteoclastogenesis. Endocrinol. Metab. (Seoul), 30 (1), 35–44. DOI: 10.3803/EnM.2015.30.1.35. PMID: 25827455; PMCID: PMC4384681.
- Bukau, B., Horwich, A. L. (1998). The Hsp70 and Hsp60 chaperone machines. Cell, 92 (3), 351–366. DOI: 10.1016/s0092-8674(00)80928-9. PMID: 9476895.
- Butterfield, J. H., Ackerman, S. J., Scott, R. E., Pierre, R. V., Gleich, G. J. (1984). Evidence for secretion of human eosinophil granule major basic protein and Charcot-Leyden crystal protein during eosinophil maturation. Exp. Hematol., 12 (3), 163–170.
- Carlson, B. (2004). Human Embryology and Developmental Biology. 3rd ed. Mosby. 512 pp.
- Caruso Bavisotto, C., Alberti, G., Vitale, A. M., Paladino, L., Campanella, C., Rappa, F., et al., (2020). Hsp60p post-translational modifications: Functional and pathological consequences. Front. Mol. Biosci., 7, 95.
- Chun, J. N., Choi, B., Lee, K. W., Kang, D. H., Lee, J. Y., Song, I. S. et al. (2010). Cytosolic Hsp60 is involved in the NF-kappaB-dependent survival of cancer cells via IKK regulation. PLoS One, 5 (3), e9422.
- Derijcke, A., Eerens, A., Carels, C. (1996). The incidence of oral clefts: A review. Brit. J. Oral Maxillofac. Surg., 34 (6), 488–494.
- Hsu, S. M., Raine, L., Fanger, H. (1981). The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics. Amer. J. Clin. Pathol., 75 (6), 816–821.
- Iotsova, V., Caamaño, J., Loy, J., Yang, Y., Lewin, A., Bravo, R. (1997). Osteopetrosis in mice lacking NF-kappaB1 and NF-kappaB2. Nat Med., 3 (11), 1285–1289. DOI: 10.1038/nm1197-1285. PMID: 9359707.
- Itoh, A., Nonaka, Y., Nakakita, S. I., Yoshida, H., Nishi, N., Nakamura, T., Kamitori, S. (2020). Structures of human galectin-10/monosaccharide complexes demonstrate potential of monosaccharides as effectors in forming Charcot-Leyden crystals. Biochem. Biophys. Res. Commun., S0006-291X(20)30303-X. DOI: 10.1016/j.bbrc.2020.02.037. PMID: 32081418.
- de Kovel, C. G. F., Lisgo, S., Karlebach, G., Ju, J., Cheng, G., Fisher, S. E, Francks, C. (2017). Left-right asymmetry of maturation rates in human embryonic neural development. Biol. Psychiatry, 82 (3), 204-212. DOI: 10.1016/j.biopsych.2017.01.016. PMID: 28267988.
- Kumada, K., Fuse, N., Tamura, T., Okamori, C., Kurata, S. (2019). HSP70/DNAJA3 chaperone/cochaperone regulates NF-kappaB activity in immune responses. Biochem. Biophys. Res. Commun., 513 (4), 947–951.
- Lian, W. S., Ko, J. Y., Chen, Y. S., Ke, H. C., Wu, S. L., Kuo, C.W., Wang, F. S. (2018). Chaperonin 60 sustains osteoblast autophagy and counteracts glucocorticoid aggravation of osteoporosis by chaperoning RPTOR. Cell Death Dis., 9 (10), 938.
- Lin, F. Y., Hsiao, F. P., Huang, C. Y., Shih, C. M., Tsao, N. W.,Tsai, C. S. et al. (2014). Porphyromonas gingivalis GroEL induces osteoclastogenesis of periodontal ligament cells and enhances alveolar bone resorption in rats. PLoS One, 9 (7), e102450.
- Lewsey, J. (2006). Medical statistics: A guide to data analysis and critical appraisal. Ann. R. Coll. Surg. Engl., 88 (6), 603. DOI: 10.1308/003588406X117098g. PMCID: PMC1963749.
- Mastrianni, D. M., Eddy, R. L., Rosenberg, H. F., Corrette, S. E., Shows, T. B., Tenen, D. G., Ackerman, S. J., et al. (1992). Localization of the human eosinophil Charcot-Leyden crystal protein (lysophospholipase) gene (CLC) to chromosome 19 and the human ribonuclease 2 (eosinophil-derived neurotoxin) and ribonuclease 3 (eosinophil cationic protein) genes (RNS2 and RNS3) to chromosome 14. Genomics, 13 (1), 240–242.
- McCerery, R., Hall, L., Omanakuttan, J., Chai, H. Z., Lawrence, J., Woodward, J., Pearce, D A. (2025). Preparation protocol for epifluorescence microscopy when working close to the detection limit. Lett. Appl. Microbiol., 78 (30), ovaf026. DOI: 10.1093/lambio/ovaf026.
- Meghji, S., Lillicrap, M., Maguire, M., Gaston, J. S. H., Poole, S., Henderson, B. (2003). Human chaperonin 60 (Hsp60) stimulates bone resorption: Structure/function relationships. Bone, 33 (3), 419–425.
- Melo, R.C.N., Wang, H., Silva, T. P., Imoto, Y., Fujieda, Fukuchi, M., et al. (2020). J. Leukoc. Biol., 108 (1), 139–149.
- Moorthy, A. K., Savinova, O. V., Ho, J. Q., Wang, V. Y., Vu, D., Ghosh, G. (2006). The 20S proteasome processes NF-kappaB1 p105 into p50 in a translation-independent manner. EMBO J., 25 (9), 1945–1956. DOI: 10.1038/sj.emboj.7601081. PMID: 16619030; PMCID: PMC1456938.
- Oeckinghaus, A., Ghosh, S. (2009). The NF-kappaB family of transcription factors and its regulation. Cold Spring Harb Perspect Biol., 1 (4):a000034. DOI: 10.1101/cshperspect.a000034. PMID: 20066092; PMCID: PMC2773619.
- Ozola, L., Pilmane, M. (2025). Characterization of factors associated with tissue immunity, cellular activity and angiogenesis in children with unilateral cleft lip and palate before and during primary dentition: A comparative cross-sectional study. J. Clin. Med., 14 (14), 4952. DOI: https://doi.org/10.3390/jcm14144952
- Perkins, N. D. (2007). Integrating cell-signalling pathways with NF-kappaB and IKK function. Nat. Rev. Mol. Cell Biol., 8 (1), 49–62.
- Pilmane, M., Shine, J., Iismaa, T. P. (1998). Distribution of galanin immunoreactivity in the bronchi of humans with tuberculosis. Ann. NY Acad. Sci., 863, p. 445–449.
- Rajion, Z. A., Alwi, Z. (2007). Genetics of cleft lip and palate: A review. Malays. J. Med. Sci., 14 (1), 4–9.
- Ran, R., Lu, A., Zhang, L., Tang, Y., Zhu, H., Xu, H., Feng, Y., Han, C., Zhou, G., Rigby, A. C., Sharp, F. R. (2004). Hsp70 promotes TNF-mediated apoptosis by binding IKK gamma and impairing NF-kappa B survival signaling. Genes Dev., 18 (12), 1466–1481. DOI: 10.1101/gad.1188204. PMID: 15198984; PMCID: PMC423196.
- The Human Protein Atlas. RELA protein expression summary. https://www.proteinatlas.org/ENSG00000173039-RELA (accessed 18.06.2025).
- Selvarajah, G. T., Bonestroo, F. A. S., Kirpensteijn, J., Kik, M. J. L., van der Zee, R., van Eden, Y. (2013). Heat shock protein expression analysis in canine osteosarcoma reveals HSP60 as a potentially relevant therapeutic target. Cell Stress Chaperones, 18 (5), 607–622.
- Smarius, B., Loozen, C., Manten, W., Bekker, M., Pistorius, L., Breugem, C. (2017). Accurate diagnosis of prenatal cleft lip/palate by understanding the embryology. World J. Methodol., 7 (3), 93–100.
- Su, J. (2018). A brief history of Charcot-Leyden crystal protein/Galectin-10 research. Molecules, 23 (11), 29321.
- Suvarna, K. S., Layton, C., Bancroft, J. D. (2019). Bancroft’s Theory and Practice of Histological Techniques. Elsevier, New York, NY, USA.
- Tomizawa, H., Yamada, Y., Arima, M., Miyabe, Y., Fukuchi, M., Hikichi, H., et al. (2022). Galectin-10 as a potential biomarker for eosinophilic diseases. Biomolecules, 12 (10), 1385.
- Vaira, S., Alhawagri, M., Anwisye, I., Kitaura, H., Faccio, R., Novack, D. V., et al., (2008). RelA/p65 promotes osteoclast differentiation by blocking a RANKL-induced apoptotic JNK pathway in mice. J. Clin. Invest., 118 (6), 2088–2097.
- Vayssier, C., Mayrand, D., Grenier, D. (1994). Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by western immunoblotting analysis. FEMS Microbiol. Lett., 121 (3), 303–307. DOI: 10.1111/j.1574-6968.1994.tb07117.x. PMID: 7926686.
- Vyas, T., Gupta, P., Kumar, S., Gupta, R., Gupta, T., Singh, H. P. (2020). Cleft of lip and palate: A review. J. Family Med. Prim. Care, 9 (6), 2621–2625. DOI: 10.4103/jfmpc.jfmpc_472_20. PMID: 32984097; PMCID: PMC7491837.
- Wang, J., Jin, L., Li, X., Deng, H., Chen, Y., Lian, Q., Ge, R., Deng, H. (2013). Gossypol induces apoptosis in ovarian cancer cells through oxidative stress. Mol. Biosyst., 9 (6), p. 1489–1497.
- Wang, W. J., Wang, C. L.,Ding, S. L. (2021). Advances in immuno-logical research of amphioxus. Dev. Comp. Immunol., 118, 103992.
- Yilmaz, H. N., Ozbilen, E.O., Ustun, T. (2019). The prevalence of cleft lip and palate patients: A single-centre experience for 17 years. Turk. J. Orthod., 32 (3), 139–144.
DOI: https://doi.org/10.2478/prolas-2026-0030 | Journal eISSN: 2255-890X (formerly 1407-009X) | Journal ISSN: 1407-009X
Language: English
Page range: 289 - 299
Submitted on: Jun 21, 2026
Accepted on: Sep 14, 2026
Published online: Nov 3, 2026
Published in print: Oct 1, 2026
Published by: Latvian Academy of Sciences
In partnership with: Paradigm Publishing Services
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© 2026 Māris Puzāks, Ilze Akota, Māra Pilmane, published by Latvian Academy of Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.