Analysis of Latvian and Belarusian Oak (Quercus robur L.) Population Provenance and Genetic Structure Using Chloroplast Markers

Padutov, Vladimir E.; Baranov, Oleg Y.; Kagan, Dmitry I.; Razumova, Olga A.; Veinberga, Ilze; Baumanis, Imants; Ruņģis, Dainis Edgars

doi:10.2478/prolas-2021-0037

Abstract

In this study, oak specific chloroplast simple sequence repeat (SSR) markers were used to analyse Latvian and Belarusian oak (Quercus robur L.) population provenance and genetic structure. Chloroplast haplotypes were compared between Latvian and Belarusian pedunculate oak, and several common haplotypes were identified. The SSR haplotypes were compared to previously reported PCR-RFLP haplotypes, and haplotypes from the A (eastern European) and C (central European) lineages were identified. C lineage haplotypes were only found in the western region of Latvia. Haplotypes shared between Latvia and Belarus were all from the A lineage. Despite the much smaller population size of oak in Latvia in comparison to Belarus, the level of genetic diversity identified using the chloroplast SSR markers is similar. Provenance trials will need to be established in order to determine the suitability of Belarusian oak reproductive material for deployment in Latvia. Based on the results from this comparison of chloroplast haplotypes, as well as climatic similarities, it is probable that Belarusian oak material will be better suited to the eastern regions of Latvia compared to the western regions of Latvia.

References

Bergot, M., Cloppet, E., Perarnaud, V., Deque, M., Marcais, B., Desprez-Loustau, M.-L. (2004). Simulation of potential range expansion of oak disease caused by Phytophthoracinnamomi under climate change. Global Change Biology, 10, 1539–1552.10.1111/j.1365-2486.2004.00824.x
Search in Google Scholar Back to article
Brewer, S., Cheddadi, R., deBeaulieu, J. L., Reille, M. (2002). The spread of deciduous Quercus throughout Europe since the last glacial period. Forest Ecol. Manag., 156, 27–48.10.1016/S0378-1127(01)00646-6
Search in Google Scholar Back to article
Csaikl, U. M., Glaz, I., Baliuckas, V., Petit, R. J., and Jensen, J. S. (2002a). Chloroplast DNA variation of white oak in the Baltic countries and Poland. Forest Ecol. Manag., 156, 211–222.10.1016/S0378-1127(01)00644-2
Search in Google Scholar Back to article
Csaikl, U. M., Burg, K., Fineschi, S., König, A. O., Mátyás, G., Petit, R. J. (2002b). Chloroplast DNA variation of white oaks in the Alpine region. Forest Ecol. Manag., 156, 131–145.10.1016/S0378-1127(01)00639-9
Search in Google Scholar Back to article
Deguilloux, M.-F, Dumolin-Lapègue, S., Gielly, L., Grivet, D., Petit, R. J. (2003). A set of primers for the amplification of chloroplast microsatellites in Quercus. Mol. Ecol. Notes, 3, 24–27.10.1046/j.1471-8286.2003.00339.x
Search in Google Scholar Back to article
Deguilloux, M.-F., Pemonge, M.-H., Petit, R. J. (2004). Use of chloroplast microsatellites to differentiate oak populations. Ann. Forest Sci., 61, 825–830.10.1051/forest:2004078
Search in Google Scholar Back to article
Demesure, B., Sodzi, N., Petit, R. J. (1995). A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol. Ecol., 4, 129–131.10.1111/j.1365-294X.1995.tb00201.x7711952
Search in Google Scholar Back to article
Doyle, J. J., Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.
Search in Google Scholar Back to article
Excoffier, L., Laval, G., Schneider, S. (2005). Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evol. Bioinform., 1, 47–50.10.1177/117693430500100003
Search in Google Scholar Back to article
Gailis, A., Smaukstelis, E. (1998). Oak and beech resources in Latvia. In: Turok, J., Kremer, A., deVries, S. (comp.). First EUFORGEN Meeting on Social Broadleaves, Bordeaux, France, 23–25 October 1997. International Plant Genetic Resources Institute, Rome, pp. 121–126.
Search in Google Scholar Back to article
Hewitt, G. M. (1999). Post-glacial re-colonization of European biota. Biol. J. Linnean Soc., 68, 87–112.10.1111/j.1095-8312.1999.tb01160.x
Search in Google Scholar Back to article
Jurkšienė, G., Baranov, O. Y., Kagan, D. I., Kovalevič-Razumova, O. A., Baliuckas, V. (2020). Genetic diversity and differentiation of pedunculate (Quercus robur) and sessile (Q. petraea) oaks. J. Forestry Res., 31, 2445–2452.10.1007/s11676-019-01043-3
Search in Google Scholar Back to article
Kovalevich, O. A., Kagan D. I., Padutov V. E. (2010). Genetic structure and gene geography of oak stands in south part of Belarus. Proc. Natl. Acad. Sci. Belarus, 4, 16–19.
Search in Google Scholar Back to article
König, A. O., Ziegenhagen, B., van Dam, B. C., Csaikl, U. M., Coart, E., Degen, B., Burg, K., de Vries, S. M. G., Petit, R. J. (2001). Chloroplast DNA variation of oaks in western Central Europe and the genetic consequences of human influences. Forest Ecol. Manag., 156, 147–166.10.1016/S0378-1127(01)00640-5
Search in Google Scholar Back to article
Peakall, R., Smouse, P. E. (2012). GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research: An update. Bioinformatics, 28, 2537–2539.
Search in Google Scholar Back to article
Perrier, X., Jacquemoud-Collet, J. P. (2006). DARwin software. http://darwin.cirad.fr/darwin (accessed 14.07.2021).
Search in Google Scholar Back to article
Petit, R. J., Csaikl, U. M., Bordács, S., Burg, K., Coart, E., Cottrell, J., Van Dam, B., Deans, J. D., Dumolin-Lapčgue, S., Fineschi, S. et al. 2002a. Chloroplast DNA variation in European white oaks phylogeography and patterns of diversity based on data from over 2600 populations. Forest Ecol. Manag., 156, 5–26.
Search in Google Scholar Back to article
Petit, R. J., Brewer, S., Bordacs, S., Burg, K., Cheddadi, R., Coart, E., Cottrell, J., Csaikl, U. M., van Dam, B. C., Deans, J. D. et al. 2002b. Identification of refugia and postglacial colonization routes of European white oaks based on chloroplast DNA and fossil pollen evidence. Forest Ecol. Manag., 156, 49–74.
Search in Google Scholar Back to article
Pliura, A., Rungis, D., Baliuckas, V. (2009). Population structure of pedunculate oak (Quercus robur L. ) in Lithuania based on analysis of chloroplast DNA haplotypes and adaptive traits. Baltic Forestry, 15, 2–12.
Search in Google Scholar Back to article
Provan, J., Powell, W., Hollingsworth, P. M. (2001). Chloroplast microsatellites: New tools for studies in plant ecology and evolution. Trends Ecol. Evol., 16 (3), 142–147.10.1016/S0169-5347(00)02097-8
Search in Google Scholar Back to article
Ruņģis, D., Korica, A., Pušpure, I., Veinberga, I. (2016). Analysis of the genetic diversity and population structure of Latvian ash (Fraxinus excelsior L. ) stands using nuclear and chloroplast SSR markers. Proc. Latvian Acad. Sci. Section B, 70 (3), 101–108.
Search in Google Scholar Back to article
Sáenz-Romero, C., O'Neill, G., Aitken, S. N., Lindig-Cisneros, R. (2021). Assisted migration field tests in Canada and Mexico: Lessons, limitations, and challenges. Forests, 12 (1), 9.
Search in Google Scholar Back to article
Stewart, J. R., Lister, A. M. (2001). Cryptic northern refugia and the origins of the modern biota. Trends Ecol. Evol., 16, 608–613.10.1016/S0169-5347(01)02338-2
Search in Google Scholar Back to article
Taberlet, P., Gielly, L., Pautou, G., Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol., 17, 1105–1109.10.1007/BF000371521932684
Search in Google Scholar Back to article
Teacher A. G. F., Griffiths D. J. (2011). HapStar: Automated haplotype network layout and visualisation. Mol. Ecol. Res., 11 (1), 151–153.10.1111/j.1755-0998.2010.02890.x21429113
Search in Google Scholar Back to article
Zunde, M. (1999). Mežainuma un koku sugu sastāva pārmaiņu dinamika un to galvenie ietekmējošie faktori Latvijas teritorijā [Dynamics of changes in forest coverage and species and the major factors influencing these in the territory of Latvia]. In: Strods, H. (ed.). Latvijas mežu vēsture līdz 1940. gadam [History of Latvian Forests until 1940]. World Wildlife Fund, Rīga, 111.–114. lpp. (in Latvian).
Search in Google Scholar Back to article

Analysis of Latvian and Belarusian Oak (Quercus robur L.) Population Provenance and Genetic Structure Using Chloroplast Markers

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