Induction of Polyploidy in Giant Miscanthus (Miscanthus × Giganteus Greef Et Deu.)

Melnychuk, Oleksandr V.; Ozheredov, Serhiy; Rakhmetov, Dzhamal B.; Shysha, Olena O.; Rakhmetova, Svitlana O.; Yemets, Alla I.; Blume, Yaroslav B.

doi:10.2478/prolas-2020-0032

Abstract

Results of obtaining miscanthus polyploid lines using both classic and new dinitroanilines to improve productivity of biomass as well as quality for biofuel production are represented. All of compounds applied in the study appeared to be able to induce poyploidy in Miscanthus × giganteus.It was found that new antimitotics have a lower phytotoxicity level compared to classic dinitroani-lines. Ploidy level of obtained lines was confirmed by cytological studies using light microscopy. These lines are acclimatised for growing in open soil conditions. Currently, morphometric parameters of selected lines are being studied and analysed.

References

Armstrong, C. L., Green, C. E., Phillips, R. L., Stucker, R. E. (1985). Genetic control of plant regeneration from maize tissue cultures. Maize Genet. Cooperation Newslett., 92–93.
Search in Google Scholar Back to article
Banerjee, M., Roy, D., Bhattacharyya, B., Basu, G. (2007). Differential colchicine-binding across eukaryotic families: The role of highly conserved Pro268b and Ala248b residues in animal tubulin. FEBS Lett., 581, 5019–5023.10.1016/j.febslet.2007.09.04717927988
Search in Google Scholar Back to article
Birchler, J. A., Auger, D. L., Riddle, N. C. (2003). In search of the molecular basis of heterosis. Plant Cell., 15, 2236–2239.10.1105/tpc.15103054026914523245
Search in Google Scholar Back to article
Bilandzija, N., Vocan, N., Leto, J., Jurisic, V., Grubor, M., Matin, A., Gersic, A., Krisca, T. (2018). Yield and biomass composition of Miscanthus x giganteus in the mountain area of Croatia. J. Transactions FAMENA,42, 51–60.10.21278/TOF.42Si105
Search in Google Scholar Back to article
Blakeslee, A., Avery, G. (1937). Methods of inducing doubling of chromosomes in plants, J. Heredity,28, 393–411.10.1093/oxfordjournals.jhered.a104294
Search in Google Scholar Back to article
Britsun, V. M., Yemets, A. I., Lozinskii, M. O., Blume, Ya. B. (2009). 2,6-Dinitroanilines: synthesis, herbicidal and antiprotozoan properties. Ukr. Bioorg. Acta,1, 16–27 (in Ukrainian)
Search in Google Scholar Back to article
Chramiec-Glabik, A., Grabowska-Joashimiak, A., Sliwinska, E., Legutko, J., Kula, A. (2012). Cytogenetic analysis of Miscanthus × giganteus and its parent forms. Caryologia, 65, 234–242.10.1080/00087114.2012.740192
Search in Google Scholar Back to article
Christian, D. G., Haase, E. (2001). Agronomy of Miscanthus. In: Miscanthus for Energy and Fibre. James &James, London, pp. 21–45.
Search in Google Scholar Back to article
Clifton-Brown, J., Chiang, Y. C., Hodkinson, T. R. (eds.) (2008). Miscanthus: Genetic resources and breeding potential to enhance bioenergy production. In: Aspects of Applied Biology. Biomass and Energy Crops. Association of Applied Biologists, Warwick, University of York, UK., pp. 273–294.
Search in Google Scholar Back to article
Dalton S. J. (2013). Biotechnology of Miscanthus. In: Biotechnology of Neglected and Underutilized Crops. Springer Science Business Media, Dordrecht, pp. 243–294.10.1007/978-94-007-5500-0_11
Search in Google Scholar Back to article
Dalton, S. J., Bettany, A. J., Timms, E., Morris, P. (1999). Co-transformed, diploid Lolium perenne (perennial ryegrass), Lolium multiflorum (Italian ryegrass) and Lolium temulentum (darnel) plants produced by micro-projectile bombardment. Plant Cell Rep., 18, 721–726.10.1007/s002990050649
Search in Google Scholar Back to article
Glowacka, K., Jezowski, S. (2009). Genetic and nongenetic factors influencing callus induction in Miscanthus sinensis (Anderss.) anther cultures. J. Appl. Genet., 50, 341–345.10.1007/BF0319569219875884
Search in Google Scholar Back to article
Glowacka, K., Jezowski, S., Kaczmarek, Z., (2010a). The effects of geno-type, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species. Plant Cell, Tiss. Org. Cult., 102, 79–86.10.1007/s11240-010-9708-6
Search in Google Scholar Back to article
Głowacka K., Jeżowski S., Kaczmarek Z. (2010b). In vitro induction of polyploidy by colchicine treatment of shoots and preliminary characterisation of induced polyploids in two Miscanthus species. Indust. Crops Prod., 32 (2), 88–96.10.1016/j.indcrop.2010.03.009
Search in Google Scholar Back to article
Gubisova, M., Gubis, J., Zofajova, A., Mihalik, D., Kraic, J. (2013). Enhanced in vitro propagation of Miscanthus × giganteus. Indust. Crop Prod., 41, 279–282.10.1016/j.indcrop.2012.05.004
Search in Google Scholar Back to article
Holme, I. B., Petersen, K. K. (1996). Callus induction and plant regeneration from different explants types of Miscanthus × ogiformis Honda ‘Giganteus’. Plant Cell Tiss. Org. Cult., 45, 43–52.10.1007/BF00043427
Search in Google Scholar Back to article
Holme, I., Krogstrup, P., Hansen, J. (1997). Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus × ogiformis Honda ‘Giganteus’ as affected by proline. Plant Cell Tiss. Org. Cult., 50, 203–210.10.1023/A:1005981300847
Search in Google Scholar Back to article
Kundelchuk, O. P., Tarasenko, L. V., Blume, Ya. B. (2002). Influence of amiprophosmethyl on the root cell structure in the herbicide-sensitive and resistant lines of Nicotiana plumbaginifolia. Russ. J. Plant Physiol., (3), 381–386.10.1023/A:1015501304476
Search in Google Scholar Back to article
Lewandowski, I. (1997). Micropropagation of Miscanthus × giganteus. Biotech. Agr. Forest., 39, 241–255.10.1007/978-3-662-07774-0_16
Search in Google Scholar Back to article
Melnychuk, O. V., Ozheredov, S. P., Sekan, O. S., Bayer, G. Ya., Shysha, O. M., Yemets, A. I. (2015). Development and application of method for miscanthus in vitro culture establishment. Factors Exp. Evol. Organisms, 17, 209–212 (in Ukrainian).
Search in Google Scholar Back to article
Melnychuk, O. V., Ozheredov, S. P., Rakhmetov, D. B., Yemets, A. I., Blume, Ya. B. (2016). Screening of nitroanilines for their affinity to miscanthus α-tubulin for further in vitro polyploidization of miscanthus species. Factors Exp. Evol. Organisms, 18, 212–216 (in Ukrainian).
Search in Google Scholar Back to article
Morejohn, L. C., Fosket, D. E. (1991). The biochemistry of compounds with anti-microtubule activity in plant cells. Pharm. Ther., 51, 217–230.10.1016/0163-7258(91)90078-Z
Search in Google Scholar Back to article
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15, 473–497.10.1111/j.1399-3054.1962.tb08052.x
Search in Google Scholar Back to article
Ozheredov, S. P., Yemets, A. I., Brytsun, V. M., Ozheredova, I. P., Lozynskiy, M. O., Blume, Ya. B. (2009). Screening of new 2,4- and 2,6-dinitroaniline derivates on phytotoxicity and antimitotic activity. Cytol. Genetics, 43 (5), 297–304.10.3103/S0095452709050016
Search in Google Scholar Back to article
Ozheredov, S. P., Yemets, A. I., Lytvyn, D. I., Brytsun, V. M., Schwartau, V. V., Lozynskiy, M. O., Blume, Ya. B. (2010). New 2,6-dinitroaniline compounds with antimitotic mode of action and their synergistic activity in compositions with graminicides. Cytol. Genetics, 5, 306–312.10.3103/S0095452710050087
Search in Google Scholar Back to article
Pausheva, Z. P. (1988). Workshop on Plant Cytology [Паушева 3. П. Пра-ктикум no цитологии растений]. Agropromizdat, Moscow. 271 pp. (in Russian)
Search in Google Scholar Back to article
Petersen, K. (1997). Callus induction and plant regeneration in Miscanthus × ogiformis Honda ‘Giganteus’ as influenced by benzyladenine. Plant Cell Tiss. Org. Cult., 49, 137–140.10.1023/A:1005808329685
Search in Google Scholar Back to article
Petersen, K., Hagberg, P., Kristiansen, K. (2002). In vitro chromosome doubling of Miscanthus sinensis. Plant Breed., 121, 445–450.10.1046/j.1439-0523.2002.738314.x
Search in Google Scholar Back to article
Petersen, K., Hagberg, P., Kristiansen K. (2003). Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis. Plant Cell Tiss. Org. Cult., 73, 137–146.10.1023/A:1022854303371
Search in Google Scholar Back to article
Plazek, A., Dubert, F., Zur, I., Waligorski, P. (2007). In vitro culture of Miscanthus × giganteus. Zeszyty Problemowe Postepow Nauk Rolniczych., 523, 175–184.
Search in Google Scholar Back to article
Plazek, A., Dubert, F. (2010). Improvement of medium for Miscanthus ×giganteus callus induction and plant regeneration. Acta Biol. Cracov. Ser. Bot., 52 (1), 105×110.10.2478/v10182-010-0013-9
Search in Google Scholar Back to article
Rayburn, A., Crawford, J., Rayburn, C., Juvik, J. (2009). Genome size of three Miscanthus species. Plant Mol. Biol. Rep., 27, 184–188.10.1007/s11105-008-0070-3
Search in Google Scholar Back to article
Rhodes, C. A., Green, C. E., Phillips, R. L. (1986). Factors affecting tissue culture initiation from maize tassel. Plant Sci., 46, 225–232.10.1016/0168-9452(86)90196-2
Search in Google Scholar Back to article
Robinson, J., Engelborghs, Y. (1982). Tubulin polymerization in dimethyl sulfoxide. J. Biol. Chem., 257 (10), 5367–5371.10.1016/S0021-9258(19)83785-7
Search in Google Scholar Back to article
Somerville, S., Youngs, H., Tailor, S., Davis, S. C., Long, S. P. (2010). Feedstocks for lignocellulosic biofuels. Science, 329 (5993), 790–792.10.1126/science.1189268
Search in Google Scholar Back to article
Spasevska I., Ayoub A. T., Winter P., Preto J., Wong G. K.-S., Dumontet C., Tuszynski J. A. (2017). Modeling the Colchicum autumnale tubulin and a comparison of its interaction with colchicine to human tubulin. Int. J. Mol. Sci.1676, 18.10.3390/ijms18081676557806628767055
Search in Google Scholar Back to article
Thomas, H. (1993). Chromosome manipulation and polyploidy. In: Chromo-some Manipulation and Polyploidy in Plant Breeding: Principles and Prospects. Hayward, M. D., Bosemark, N. O., Romagosa, T. (eds.). Chapman and Hall, London, pp. 79–92.10.1007/978-94-011-1524-7_7
Search in Google Scholar Back to article
Tytko, R., Kalilichenko, V. (2010). Renewable Energy Sources (Poland experience for Ukraine). OWG, Warshaw. 533 pp. (in Ukrainian).
Search in Google Scholar Back to article
Weijde, T., Alvim Kamei, C. L., Torres, A. F., Vermerris, W., Dolstra, O., Visser, R. G., Trindade, L. M. (2013). The potential of C4 grasses for cellulosic biofuel production. Front. Plant Sci., 4, 107.10.3389/fpls.2013.00107
Search in Google Scholar Back to article
Weijde, T., Kiesel, A., Iqbal, Y., Muylle, H., Dolstra, O., Visser, R.G., Lewandowski, I., Trindade, L. M. (2017), Evaluation of Miscanthus sinensis biomass quality as feedstock for conversion into different bioenergy products. GCB Bioenergy, 9, 176–190.10.1111/gcbb.12355
Search in Google Scholar Back to article
Wan, Y., Ducan, D. R., Rayburn, A. L., Widholm, J. M. (1991). The use of antimicrotubule herbicides for the production of doubled haploid plants from anther culture derived maize callus. Theor. Appl. Genetics.,81, 205–211.10.1007/BF0021572424221204
Search in Google Scholar Back to article
Wang, X., Yamada, T., Kong, F. J., Abe, Y., Hoshino, Y., Sato, H., Takamizo, T., Kanazawa, A., Yamada, T. (2011). Establishment of an efficient in vitro culture and particle bombardment-mediated transformation systems in Miscanthus sinensis Anderss., a potential bioenergy crop. GCB Bioenergy, 3 (4), 322–332.10.1111/j.1757-1707.2011.01090.x
Search in Google Scholar Back to article
Yemets, A. I., Blume, Ya. B. (1999). Plant resistance to herbicides with antimicrotubule mode of action: From natural mutants to gene transfer. Russ. J. Plant Physiol., 46 (6), 899–907 (in Russian).
Search in Google Scholar Back to article
Yemets, A. I., Blume, Ya. B. (1999). Plant resistance to herbicides with antimicrotubule mode of action: From natural mutants to gene transfer [Емец, A. И., Блюм, Я. Б. Устойчивость растений к гербицидам с антимикротрубочковым механизмом действия: от природных мутантов до переноса генов]. Russ. J. Plant Physiol., 46 (6), 899–907 (in Russian).
Search in Google Scholar Back to article
Yemets, A. I., Blume, Ya. B. (2008). Progress in plant polyploidization based on antimicrotubular drugs. The Open Horticulture J., 1 (1), 15–20.10.2174/1874840600801010015
Search in Google Scholar Back to article
Yu, C. Y., Kim, H. S., Burn, A. L., Widholm, J. M., Juvik, J. A. (2009). Chromosome doubling of the bioenergy crop, Miscanthus × giganteus. GCB Bioenergy, 1, 404–412.10.1111/j.1757-1707.2010.01032.x
Search in Google Scholar Back to article
Zhang, Q. X., Sun, Y., Hu, H. K., Chen, B., Hong, C. T., Guo, H. P., Pan, Y. H., Zheng, B. S. (1991). Micropropagation and plant regeneration from embryogenic callus of Miscanthus sinensis. In Vitro Cell Dev. Biol. Plant., 48, 50–57.10.1007/s11627-011-9387-y
Search in Google Scholar Back to article
Zilbervarg, I. R., Mitrofanova, I. V., Mitrofanova, O. V., Rabotyagov, V. D., Blume, Ya. B. (1997). Using oryzalin and aminophosmethyl obtaining issope and nep polyploid forms in vitro. Cell Biol. Int.,21 (12), 914–915.
Search in Google Scholar Back to article
Zili, Y., Puhua, Z., Chengcai, C., Xiang, L., Wenzhong, T., Li, W., Shouyun, C., Zuoshun, T. (2004). Establishment of genetic transformation system for Miscanthus sacchariflorus and obtaining of its transgenic plants. High Tech. Lett., 10, 27–31.
Search in Google Scholar Back to article

Induction of Polyploidy in Giant Miscanthus (Miscanthus × Giganteus Greef Et Deu.)

Abstract

Paradigm

My account