Comparative quality assessment of the spring and autumn – harvest spruce sprouts
By: Klinta Karklina and Liene Ozola

References
- Aalto, J., Porcar‐Castell, A., Atherton, J., Kolari, P., Pohja, T., Hari, P., Nikinmaa, E., PetÄJÄ, T., & Bäck, J. (2015). Onset of photosynthesis in spring speeds up monoterpene synthesis and leads to emission bursts. Plant, Cell & Environment, 38(11), 2299–2312. https://doi.org/10.1111/pce.12550
- Adouni, K., Mekhelfi, T., Zaoui-Djelloul Daouadji, M., & Achour, L. (2018). Decoction, Infusion and Ethanolic Extract of Juncus acutus Rhizome: Phytochemical Content and Antioxidant Properties. Retrieved http://scholarsresearchlibrary.com/archive.html]
- Amboulou Badinga, A.S, Enzonga Yoca, J.A, Mvoula Tsieri, M.D. (2023). Biochemical Characterization of Four Leafy Vegetables Collected in the Northern Zone of Brazzaville (Republic of Congo). EAS Journal of Nutrition and Food Sciences, 5(1), 1–12. https://doi.org/10.36349/easjnfs.2023.v05i01.001
- Bakó, E., Böszörményi, A., Vargáné Szabó, B., Engh, M.A., Hegyi, P., Ványolós, A., & Csupor, D. (2024). Chemometric analysis of monoterpenes and sesquiterpenes of conifers. Frontiers in Plant Science, 15. https://doi.org/10.3389/fpls.2024.1392539
- Bhuiyan, N.H., Selvaraj, G., Wei, Y., & King, J. (2009). Role of lignification in plant defense. Plant Signaling & Behavior, 4(2), 158–159. https://doi.org/10.4161/psb.4.2.7688
- Buyel, J.F. (2016). Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts. Journal of Visualized Experiments, (110). https://doi.org/10.3791/53940
- Caenn, R., Darley, H.C.H., & Gray, G.R. (2011). Equipment and Procedures for Evaluating Drilling Fluid Performance. In Composition and Properties of Drilling and Completion Fluids (pp. 91–135). Elsevier. https://doi.org/10.1016/B978-0-12-383858-2.00003-2
- Camargo, S.D., Fernandes, I.A., Do Nascimento, L.H., Puton, B.M.S., Cansian, R.L., Steffens, C., Zeni, J., & Paroul, N. (2025). Biological activities of essential oil and leaf extracts from Erva Baleeira (Cordia verbenacea DC). Food and Humanity, 4, 100598. https://doi.org/10.1016/j.foohum.2025.100598
- Chociej, P., Foss, K., Jabłońska, M., Ustarbowska, M., & Sawicki, T. (2024). The Profile and Content of Polyphenolic Compounds and Antioxidant and Anti-Glycation Properties of Root Extracts of Selected Medicinal Herbs. Plant Foods for Human Nutrition, 79(2), 468–473. https://doi.org/10.1007/s11130-024-01180-z
- Dikamu, M., Ezez, D., Birhanu, H., Mamo, A., & Tefera, M. (2025). Analysis of phytochemical constituents using GC–MS, evaluation of antioxidants and antibacterial activities of Maerua oblongifolia root bark extracts. Discover Applied Sciences, 7(5), 417. https://doi.org/10.1007/s42452-025-06939-w
- Ditmarová, Ľ., Kmeť, J., Leštianska, A., & Střelcová, K. (2008). Analysis of physiological parameters of spruce trees as indicators of spruce dieback in the Spiš region. 35(1).
- Dobravalskytė, D., Venskutonis, P.R., & Talou, T. (2012). Antioxidant properties and essential oil composition of Calamintha grandiflora L. Food Chemistry, 135(3), 1539–1546. https://doi.org/10.1016/j.foodchem.2012.05.094
- Effah, E., Svendsen, L., Barrett, D.P., & Clavijo McCormick, A. (2022). Exploring plant volatile-mediated interactions between native and introduced plants and insects. Scientific Reports, 12(1), 15450. https://doi.org/10.1038/s41598-022-18479-z
- Esposito, R., Lusini, I., Večeřová, K., Holišová, P., Pallozzi, E., Guidolotti, G., Urban, O., & Calfapietra, C. (2016). Shoot-level terpenoids emission in Norway spruce (Picea abies) under natural field and manipulated laboratory conditions. Plant Physiology and Biochemistry, 108, 530–538. https://doi.org/10.1016/j.plaphy.2016.08.019
- Fober, H. (1987). A study of pH gradients in crowns of spruce and pine relative to flowering. Forest Ecology and Management, 19(1–4), 129–134. https://doi.org/10.1016/0378-1127(87)90018-1
- Furnell, H., Wenger, J., Wingler, A., Kilcawley, K.N., Mannion, D.T., Skibinska, I., & Kammer, J. (2024). Identification of volatile organic compounds emitted by Sitka spruce and determination of their emission pathways and fluxes. https://doi.org/10.5194/egusphere-2024-154
- Gulcin, İ. (2025). Antioxidants: a comprehensive review. Archives of Toxicology, 99(5), 1893–1997. https://doi.org/10.1007/s00204-025-03997-2
- Haring, D.A., Suter, D., Amrhein, N., & Luscher, A. (2007). Biomass Allocation is an Important Determinant of the Tannin Concentration in Growing Plants. Annals of Botany, 99(1), 111–120. https://doi.org/10.1093/aob/mcl227
- Holzke, C., Hoffmann, T., Jaeger, L., Koppmann, R., & Zimmer, W. (2006). Diurnal and seasonal variation of monoterpene and sesquiterpene emissions from Scots pine (Pinus sylvestris L.). Atmospheric Environment, 40(17), 3174–3185. https://doi.org/10.1016/j.atmosenv.2006.01.039
- Ilek, A., Gąsecka, M., Magdziak, Z., Saitanis, C., & Siegert, C.M. (2024). Seasonality Affects Low-Molecular-Weight Organic Acids and Phenolic Compounds’ Composition in Scots Pine Litterfall. Plants, 13(10), 1293. https://doi.org/10.3390/plants13101293
- Juvany, M., Müller, M., & Munné-Bosch, S. (2013). Photo-oxidative stress in emerging and senescing leaves: a mirror image? Journal of Experimental Botany, 64(11), 3087–3098. https://doi.org/10.1093/jxb/ert174
- Karklina, K. (2024). Evaluation of energy drink based on spruce sprout, fruit juice and cold brew coffee: master thesis food and beverage tehnology Latvia University of Life sciences and technologies Food technology faculty. Jelgava pp.-67 (in English).
- Karklina, K. (2022). Spruce and pine bud development: bachelor thesis for degree in food and bevergae technology. Latvia University of Life sciences and technologies Food technology faculty. Jelgava pp.-34 (in Latvian).
- Karklina, K., & Ozola, L. (2023). Evaluation of Pine Cone Syrups and Changes in Physical Parameters during Storage. Rural Sustainability Research, 49(344). https://doi.org/10.2478/plua-2023-0007
- Karklina, K., Ozola, L., & Ibrahim, M.N. (2024). Development of innovative energy drink based on cold brew-spruce sprout and its comparison to commercial energy drinks. Agronomy Research, 22(Special Issue 1). https://doi.org/10.15159/AR.24.024
- Karlsson, T., Klemedtsson, L., Rinnan, R., & Holst, T. (2021). Leaf-Scale Study of Biogenic Volatile Organic Compound Emissions from Willow (Salix spp.) Short Rotation Coppices Covering Two Growing Seasons. Atmosphere, 12(11), 1427. https://doi.org/10.3390/atmos12111427
- Katrevics, J., Neimane, U., Dzerina, B., Kitenberga, M., Jansons, J., & Jansons, A. (2018). Environmental factors affecting formation of lammas shoots in young stands of Norway spruce (Picea abies Karst.) in Latvia. IForest -Biogeosciences and Forestry, 11(6), 809–815. https://doi.org/10.3832/ifor2539-011
- Korolyova, N., & Turčáni, M. (2024). The factors behind spruce resistance to bark beetle attack. Online. Doctoral theses, Dissertations. Praha: Czech University of Life Sciences Prague, Faculty of Forestry and Wood Sciences. Czech Epublic, Praha, pp.138.. Available from: https://theses.cz/id/gkyrna/.
- Li, Y., Sun, Y., Jiang, J., & Liu, J. (2019). Spectroscopic determination of leaf chlorophyll content and color for genetic selection on Sassafras tzumu. Plant Methods, 15(1), 73. https://doi.org/10.1186/s13007-019-0458-0
- Linkosalo, T., Heikkinen, J., Pulkkinen, P., & Mäkipää, R. (2014). Fluorescence measurements show stronger cold inhibition of photosynthetic light reactions in Scots pine compared to Norway spruce as well as during spring compared to autumn. Frontiers in Plant Science, 5. https://doi.org/10.3389/fpls.2014.00264
- Luoranen, J. (2018). Autumn versus spring planting: the initiation of root growth and subsequent field performance of Scots pine and Norway spruce seedlings. Silva Fennica, 52(2). https://doi.org/10.14214/sf.7813
- Lybeer, B., Koch, G., Van Ackef, J., Goetghebeur, P. (2006). Lignification and Cell Wall Thickening in Nodes of Phyllostachys viridiglaucescens and Phyllostachys nigra. Annals of Botany, 97(4), 529–539. https://doi.org/10.1093/aob/mcl016
- Michelberger, T., Mezzadrelli, E., Bellan, A., Perin, G., & Morosinotto, T. (2025). The xanthophyll cycle balances photoprotection and photosynthetic efficiency in the seawater alga Nannochloropsis oceanica. Plant Physiology, 198(3). https://doi.org/10.1093/plphys/kiaf301
- Montejano-Ramírez, V., Ávila-Oviedo, J.L., Campos-Mendoza, F.J., & Valencia-Cantero, E. (2024). Microbial Volatile Organic Compounds: Insights into Plant Defense. Plants, 13(15), 2013. https://doi.org/10.3390/plants13152013
- Morais, M.C., Cabral, J.A., & Gonçalves, B. (2022). Seasonal Variation in Selected Biochemical Traits in the Leaves of Co-Occurring Invasive and Native Plant Species under Mediterranean Conditions. Plants, 11(9), 1171. https://doi.org/10.3390/plants11091171
- Muflihah, Y.M., Gollavelli, G., & Ling, Y.-C. (2021). Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs. Antioxidants, 10(10), 1530. https://doi.org/10.3390/antiox10101530
- Nisca, A., Ștefănescu, R., Stegăruș, D.I., Mare, A.D., Farczadi, L., & Tanase, C. (2021). Phytochemical Profile and Biological Effects of Spruce (Picea abies) Bark Subjected to Ultrasound Assisted and Microwave-Assisted Extractions. Plants, 10(5), 870. https://doi.org/10.3390/plants10050870
- Paciolla, C., Fortunato, S., Dipierro, N., Paradiso, A., De Leonardis, S., Mastropasqua, L., & de Pinto, M.C. (2019). Vitamin C in Plants: From Functions to Biofortification. Antioxidants, 8(11), 519. https://doi.org/10.3390/antiox8110519
- Pastori, G.M., Kiddle, G., Antoniw, J., Bernard, S., Veljovic-Jovanovic, S., Verrier, P.J., Noctor, G., & Foyer, C.H. (2003). Leaf Vitamin C Contents Modulate Plant Defense Transcripts and Regulate Genes That Control Development through Hormone Signaling[W]. The Plant Cell, 15(4), 939–951. https://doi.org/10.1105/tpc.010538
- Pei, D., Wang, A., Shen, L., & Wu, J. (2025). Research on the Emission of Biogenic Volatile Organic Compounds from Terrestrial Vegetation. Atmosphere, 16(7), 885. https://doi.org/10.3390/atmos16070885
- Pozzo, L., Raffaelli, A., Ciccone, L., Zabini, F., Vornoli, A., Calderone, V., Testai, L., & Meneguzzo, F. (2025). Conifer By-Products Extracted Using Hydrodynamic Cavitation as a Convenient Source of Phenolic Compounds and Free Amino Acids with Antioxidant and Antimicrobial Properties. Molecules, 30(13), 2722. https://doi.org/10.3390/molecules30132722
- Pramsohler, M., Lichtenberger, E., & Neuner, G. (2022). Seasonal Xylem Sap Acidification Is Governed by Tree Phenology, Temperature and Elevation of Growing Site. Plants, 11(15), 2058. https://doi.org/10.3390/plants11152058
- Puoci, F., Iemma, F., Spizzirri, U.G., Restuccia, D., Pezzi, V., Sirianni, R., Manganaro, L., Curcio, M., Parisi, O.I., Cirillo, G., & Picci, N. (2011). Antioxidant Activity of a Mediterranean Food Product: “Fig Syrup.” Nutrients, 3(3), 317–329. https://doi.org/10.3390/nu3030317
- Qasim, M., Islam, W., Rizwan, M., Hussain, D., Noman, A., Khan, K.A., Ghramh, H.A., & Han, X. (2024). Impact of plant monoterpenes on insect pest management and insect-associated microbes. Heliyon, 10(20), e39120. https://doi.org/10.1016/j.heliyon.2024.e39120
- Resente, G., & Crivellaro, A. (2025). Environmental Impacts on Plant Cell Wall Lignification. Journal of Bioresources and Bioproducts, 10(1), 4–6. https://doi.org/10.1016/j.jobab.2024.11.001
- Rumpf, J., Burger, R., & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules, 233, 123470. https://doi.org/10.1016/j.ijbiomac.2023.123470
- Sá, C., Brígido, C., Fidalgo, C., Pires, A., Alves, A., Figueira, E., & Cardoso, P. (2025). Influence of Plant Developmental Phase and Irrigation Level on Cultivable Microbiome of Maize Root. Biology, 14(12), 1694. https://doi.org/10.3390/biology14121694
- Safari-Khozani, A., Rezaei, M., Tunç, Y., & Khadivi, A. (2025). Biochemical, antioxidant activity, and phenological diversity among Berberis progenies in the F1 population. Scientific Reports, 15(1), 24967. https://doi.org/10.1038/s41598-025-11083-x
- Salminen, J.-P., Roslin, T., Karonen, M., Sinkkonen, J., Pihlaja, K., & Pulkkinen, P. (2004). Seasonal Variation in the Content of Hydrolyzable Tannins, Flavonoid Glycosides, and Proanthocyanidins in Oak Leaves. Journal of Chemical Ecology, 30(9), 1693–1711. https://doi.org/10.1023/B:JOEC.0000042396.40756.b7
- Schoss, K., Kočevar Glavač, N., & Kreft, S. (2023). Volatile Compounds in Norway Spruce (Picea abies) Significantly Vary with Season. Plants, 12(1), 188. https://doi.org/10.3390/plants12010188
- Segliņa, D. (2007). Smiltsērkšķu augļi un to pārstrādes produkti: promocijas darba kopsavilkums Inženierzinātņu doktora zinātniskā grāda iegūšanai Pārtikas zinātnē (in latvian). Jelgava, Latvia, pp.46.
- Semeniuc, C.A., Rotar, A., Stan, L., Pop, C.R., Socaci, S., Mireşan, V., & Muste, S. (2016). Characterization of pine bud syrup and its effect on physicochemical and sensory properties of kefir. CyTA - Journal of Food, 14(2), 213–218. https://doi.org/10.1080/19476337.2015.1085905
- Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2019). Response of Phenylpropanoid Pathway and the Role of Polyphenols in Plants under Abiotic Stress. Molecules, 24(13), 2452. https://doi.org/10.3390/molecules24132452
- Siddiqui, N., Rauf, A., Latif, A., & Mahmood, Z. (2017). Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa ( Nepeta bracteata Benth). Journal of Taibah University Medical Sciences, 12(4), 360–363. https://doi.org/10.1016/j.jtumed.2016.11.006
- Sims, D.A., & Gamon, J.A. (2002). Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages. Remote Sensing of Environment, 81(2–3), 337–354. https://doi.org/10.1016/S0034-4257(02)00010-X
- Skrøppa, T., & Fundova, I. (2025). Lammas shoots in Norway spruce families in short-term trials; Genetic variation, inheritance patterns and implications of micro-environmental influence. Silvae Genetica, 74(1), 1–10. https://doi.org/10.2478/sg-2025-0001
- Sudhakar, P., Latha, P., & Reddy, P.V. (2016). Plant pigments. In Phenotyping Crop Plants for Physiological and Biochemical Traits (pp. 121–127). Elsevier. https://doi.org/10.1016/B978-0-12-804073-7.00015-6
- Sutinen, S., Roitto, M., & Repo, T. (2015). Vegetative buds, needles and shoot growth of Norway spruce are affected by experimentally delayed soil thawing in the field. Forest Ecology and Management, 336, 217–223. https://doi.org/10.1016/j.foreco.2014.10.029
- Teterovska, R., Sile, I., Paulausks, A., Kovalcuka, L., Koka, R., Mauriņa, B., & Bandere, D. (2023). The Antioxidant Activity of Wild-Growing Plants Containing Phenolic Compounds in Latvia. Plants, 12(24), 4108. https://doi.org/10.3390/plants12244108
- Tienaho, J., Fidelis, M., Perämäki, A., Hed, L., Brännström, H., Rudolfsson, M., Liimatainen, J., Haapakoski, M., Marjomäki, V., Korpinen, R., Jyske, T., Matisons, M., & Kilpeläinen, P. (2026). Chemical and bioactivity profiles of needle-rich Norway spruce logging residue fractions. Industrial Crops and Products, 241, 122696. https://doi.org/10.1016/j.indcrop.2026.122696
- Tomsone, L., & Kruma, Z. (2019). Spectrophotometric analysis of pigments in horseradish by using various extraction solvents. 210–215. https://doi.org/10.22616/foodbalt.2019.023
- Ueda, H., Kikuta, Y., & Matsuda, K. (2012). Plant communication. Plant Signaling & Behavior, 7(2), 222–226. https://doi.org/10.4161/psb.18765
- Urbanovich, E.A., Afonnikov, D.A., & Nikolaev, S.V. (2021). Determination of the quantitative content of chlorophylls in leaves by reflection spectra using the random forest algorithm. Vavilov Journal of Genetics and Breeding, 25(1), 64–70. https://doi.org/10.18699/VJ21.008
- Vanhakylä, S., & Salminen, J.-P. (2023). Seasonal Variation in Plant Polyphenols and Related Bioactivities across Three Years in Ten Tree Species as Visualized by Mass Spectrometric Fingerprint Mapping. Molecules, 28(16), 6093. https://doi.org/10.3390/molecules28166093
- Wang, A., Zhang, W., & Wei, X. (2019). A review on weed detection using ground-based machine vision and image processing techniques. Computers and Electronics in Agriculture, 158, 226–240. https://doi.org/10.1016/j.compag.2019.02.005
- Wang, Y., Shao, Q., Yang, X., Su, K., Li, Z., Yang, Y., Yuan, X., & Chen, R. (2024). Diversity in Pyracantha fortuneana fruits maturity stages enables discrepancy in the phenolic compounds, antioxidant activity, and tyrosinase inhibitory activity. Journal of Food Science, 89(6), 3469–3483. https://doi.org/10.1111/1750-3841.17106
- Wilhelmy, C., Pavez, C., Bordeu, E., & Brossard, N. (2021). A Review of Tannin Determination Methods Using Spectrophotometric Detection in Red Wines and Their Ability to Predict Astringency. South African Journal of Enology and Viticulture, 42(1). https://doi.org/10.21548/42-1-3852
- Wong, C.Y.S., D’Odorico, P., Bhathena, Y., Arain, M.A., & Ensminger, I. (2019). Carotenoid based vegetation indices for accurate monitoring of the phenology of photosynthesis at the leaf-scale in deciduous and evergreen trees. Remote Sensing of Environment, 233, 111407. https://doi.org/10.1016/j.rse.2019.111407
- Xie, J., & Schaich, K.M. (2014). Re-evaluation of the 2,2-Diphenyl-1-picrylhydrazyl Free Radical (DPPH) Assay for Antioxidant Activity. Journal of Agricultural and Food Chemistry, 62(19), 4251–4260. https://doi.org/10.1021/jf500180u
- Zeppetzauer, F., Süss, R., Nadányi, R., Putz, R.F., Lisý, A., Paulik, C., Šurina, I., Strižincová, P., Huemer, K., & Kamm, B. (2023). Environmentally Friendly Extraction from Picea Abies Bark as an Approach to Accessing Valuable Antioxidants in Biorefineries. Processes, 11(7), 2145. https://doi.org/10.3390/pr11072145
- Zhai, L., Yang, J., Lu, M., Sun, T., Wang, Y., Tang, G., Wu, D., & Xu, L. (2025). Effects of Leaf Structure, Physiological Characteristics, and Chemical Properties on Phyllosphere Microorganisms Associated with Four Forage Crops in Fallow Land. Microbial Ecology, 89(1), 18. https://doi.org/10.1007/s00248-025-02638-6
- Zhang, L., Ye, G., Lin, Y., Zhou, H., & Zeng, Q. (2009). Seasonal changes in tannin and nitrogen contents of Casuarina equisetifolia branchlets. Journal of Zhejiang University SCIENCE B, 10(2), 103–111. https://doi.org/10.1631/jzus.B0820217
- Zhang, L., Zhang, S., Ye, G., & Qin, X. (2020). Seasonal variation and ecological importance of tannin and nutrient concentrations in Casuarina equisetifolia branchlets and fine roots. Journal of Forestry Research, 31(5), 1499–1508. https://doi.org/10.1007/s11676-019-00991-0
- Zhu, K., Aykas, D.P., & Rodriguez-Saona, L.E. (2022). Pattern Recognition Approach for the Screening of Potential Adulteration of Traditional and Bourbon Barrel-Aged Maple Syrups by Spectral Fingerprinting and Classical Methods. Foods, 11(15), 2211. https://doi.org/10.3390/foods11152211
- Zlobin, I.E., Kartashov, A.V., Ivanov, Y.V., Ivanova, A.I., Pashkovskiy, P.P., Gorshkova, E.N., Ashikhmina, D.A., Tatarkina, P. P., Abramova, A. A., & Kuznetsov, V. V. (2024). Auxins differentially affect growth in Scots pine and Norway spruce in spring and autumn. Environmental and Experimental Botany, 225, 105848. https://doi.org/10.1016/j.envexpbot.2024.105848
DOI: https://doi.org/10.2478/plua-2026-0005 | Journal eISSN: 2256-0939 (formerly 2255-8535) | Journal ISSN: 1407-4427 (formerly 2255-8535)
Language: English
Page range: 44 - 56
Submitted on: Apr 15, 2026
Accepted on: Jun 19, 2026
Published on: Aug 29, 2026
Published by: Latvia University of Life Sciences and Technologies
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© 2026 Klinta Karklina, Liene Ozola, published by Latvia University of Life Sciences and Technologies
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