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Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry Cover

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Open Access
|Jan 2020

Abstract

In the event of an epidemic of Legionnaires’ disease, prompt and unambiguous identification of the source of infection and immediate undertaking of repair actions is a necessary condition to limit and minimise the effects of the developing epidemic. In the classical method for determining the level of Legionella bacteria in water samples, the effectiveness of the reparative action (increase of the water temperature in the water supply system to 600C, additional chlorination) can only be confirmed after 14 days!!! Only by using the IMMS&FCM method can Legionella’s determination time be reduced to 2-4 hours, which is the most important factor in limiting the development of an epidemic.

DOI: https://doi.org/10.2478/phr-2019-0013 | Journal eISSN: 2084-0535 | Journal ISSN: 1734-7009
Language: English
Page range: 71 - 92
Submitted on: Feb 22, 2019
Accepted on: Jul 23, 2019
Published on: Jan 10, 2020
Published by: Polish Hyperbaric Medicine and Technology Society
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2020 Zbigniew Dąbrowiecki, Małgorzata Dąbrowiecka, Romuald Olszański, Piotr Siermontowski, published by Polish Hyperbaric Medicine and Technology Society
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.