Selected properties of soil humus horizon after the first and second year of the experiment
| Experimental objects | pHKCl | Hh | CEC | V | Corg | N-tot | C:N |
|---|---|---|---|---|---|---|---|
| mmol(+)·kg−1 | % | g·kg−1 | |||||
| After first year of cultivation | |||||||
| Control | 4.79 | 20.6 | 67.6 | 76.6 | 6.02 | 0.61 | 9.84 |
| SMS | 5.11 | 22.5 | 90.3 | 80.3 | 7.65 | 0.79 | 9.68 |
| After second year of cultivation | |||||||
| Control | 4.61 | 24.0 | 64.5 | 72,9 | 6.09 | 0.58 | 10.5 |
| SMS | 4.69 | 24.4 | 79.2 | 76,4 | 7.65 | 0.63 | 12.1 |
The nitrogen in separate fractions by acid hydrolysis in the A horizon of the field experiment objects
| Experimental objects | NOS | NOEH | NODH | NONH | ||||
|---|---|---|---|---|---|---|---|---|
| g·kg−1 | % N-tot | g·kg−1 | % N-tot | g·kg−1 | % N-tot | g·kg−1 | % N-tot | |
| First year of cultivation | ||||||||
| Control | 1.33 | 2.18 | 12.0 | 19.7 | 19.6 | 32.1 | 28.1 | 46.1 |
| SMS | 1.68 | 2.13 | 14.5 | 18.4 | 25.4 | 32.2 | 37.4 | 47.3 |
| Second year of cultivation | ||||||||
| Control | 1.20 | 2.07 | 11.5 | 19.8 | 16.8 | 28.9 | 28.5 | 49.1 |
| SMS | 1.82 | 2.90 | 12.0 | 19.0 | 20.5 | 32.5 | 28.2 | 44.8 |
Selected properties of spent mushroom substrate used in the experiment
| Dry matter (DM) | Corg | N-tot | C:N | pHH20 |
|---|---|---|---|---|
| g·kg−1 | ||||
| 309 | 278 | 23.3 | 11.9 | 7.25 |
Nitrogen forms and the methods of their recovery [Kalembasa, 1995; Becher, Pakuła, 2014]
| Nitrogen form (operational) | Method of recovery |
|---|---|
| N-NH4 – ammonium form | distillation from 0.25 M KCl extract after alkalization with MgO |
| N-NOx – nitrate forms (III and V): N-NO3 and N-NO2 | distillation from 0.25 M KCl extract after alkalization with N-NH4 and application of Devards reductive mixture |
| NMIN – nitrogen in mineral compounds | NMIN = N-NH4 +N-NOx |
| NORG – nitrogen in organic compounds | NORG =N-tot. – NMIN |
| NKCl | measured in 0.25 M KCl extract after mineralization of the solution |
| NOS – soluble organic nitrogen | NOS =NKCl – NMIN |
| NOEH – easily hydrolysing organic nitrogen | measured after hydrolysis in 0.25 M H2SO4 and mineralization of the solution |
| NODH – organic nitrogen difficult to hydrolyse | measured after hydrolysis in 2.5 M H2SO4 and mineralization of the solution |
| NONH – not hydrolysing organic nitrogen | measured after post-extraction and mineralization of the residues concentrated H2SO4 |
Mineral forms of nitrogen in separate fractions from the A horizon of the field experimental objects
| Experimental objects | N-NH4 | N-NO3 and N-NO2 | NMIN | |||
|---|---|---|---|---|---|---|
| g·kg−1 | % N-tot | g·kg−1 | % N-tot | g·kg−1 | % N-tot | |
| First year of cultivation | ||||||
| Control | 1.68 | 2.75 | 1.19 | 1.95 | 2.87 | 4.70 |
| SMS | 1.75 | 2.22 | 1.05 | 1.33 | 2.80 | 3.55 |
| Second year of cultivation | ||||||
| Control | 1.54 | 2.65 | 0.98 | 1.69 | 2.52 | 4.34 |
| SMS | 1.96 | 3.13 | 1.10 | 1.58 | 3.06 | 4.68 |
The coefficients of correlation between the nitrogen content in different fractions and selected properties of A horizon of the investigated soils
| Parameter | N-NH4 | N-NOx | NOS | NOEH | NODH | NONH |
|---|---|---|---|---|---|---|
| N-tot | 0.35 | 0.13 | 0.68* | 0.82* | 0.80* | 0.14 |
| Corg | 0.50 | 0.20 | 0.78* | 0.39 | 0.54 | 0.13 |
| C:N | 0.26 | 0.10 | 0.24 | −0.44 | −0.21 | −0.03 |
| pHKCl | −0.23 | −0.48 | 0.21 | 0.33 | 0.57 | −0.51 |
| Hh | 0.36 | 0.31 | 0.31 | 0.20 | −0.25 | −0.71* |
| CEC | 0.58* | 0.20 | 0.67* | 0.72* | 0.70* | −0.37 |
| V | 0.25 | −0.02 | 0.38 | 0.43 | 0.73* | −0.79* |
The mineral nitrogen forms in the investigated spent mushroom substrate
| N-NH4 | N-NO3 and N-NO2 | NMIN | |||
|---|---|---|---|---|---|
| g·kg−1 | % N-tot | g·kg−1 | % N-tot | g·kg−1 | % N-tot |
| 0.800 | 3.43 | 0.095 | 0.40 | 0.895 | 3.84 |
The nitrogen organic compounds in separate fractions by acid hydrolysis in the investigated spent mushroom substrate
| NOS | NOEH | NODH | NONH | ||||
|---|---|---|---|---|---|---|---|
| g·kg−1 | % N-tot | g·kg−1 | % N-tot | g·kg−1 | % N-tot | g·kg−1 | % N-tot |
| 2.81 | 12.0 | 4.29 | 18.4 | 4.15 | 17.8 | 11.5 | 47.8 |