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The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures Cover

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The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures

Volume 66 (2021): Issue 3 (September 2021)

By: Alexandru Petre , Mihaela Ene , Daniel Constantin Negut , Florentina Gatea and Emanuel Vamanu

Open Access

|Sep 2021

Figures & tables

Figures & Tables

Table 1

The one-way ANOVA of dry mycelium weight after irradiation and culture of the first experimental run

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	6.78	1.356	0.00468	0.068411	0.030594
100 Gy	5	7.09	1.418	0.00342	0.058481	0.026153
200 Gy	5	7.73	1.546	0.00753	0.086776	0.038807
300 Gy	5	8.07	1.614	0.00863	0.092898	0.041545
400 Gy	5	8.12	1.624	0.00433	0.065803	0.029428
ANOVA single factor (α = 0.01)
Source of variation	SS	df	MS	F	P-value	F crit.
Between groups	0.286376	4	0.071594	12.52081147	0.000029182	4.430690161
Within groups	0.114360	20	0.005718
Total	0.400736	24

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the first experimental run.

Table 2

The one-way ANOVA of dry mycelium weight after irradiation and culture of the second experimental run

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	9.20	1.840	0.00535	0.073144	0.032710
100 Gy	5	9.99	1.998	0.00452	0.067231	0.030066
200 Gy	5	10.49	2.098	0.00352	0.059330	0.026532
300 Gy	5	10.73	2.146	0.00843	0.091815	0.041060
400 Gy	5	10.35	2.070	0.00490	0.07	0.031304
ANOVA single factor (α= 0.01)
Source of variation	SS	df	MS	F	P-value	F crit.
Between groups	0.284016	4	0.071004	13.28667665	0.000019303	2.866081402
Within groups	0.106880	20	0.005344	0.10688
Total	0.390896	24

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the second experimental run.

Table 3

The one-way ANOVA of free radical scavenging potential of the mycelium extracts

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	1.3562	0.2712	0.0019	0.044382	0.019848
100 Gy	4	1.6489	0.4122	0.0011	0.034140	0.017070
200 Gy	5	2.1722	0.4344	0.0008	0.029030	0.012983
300 Gy	5	2.4386	0.4877	0.0025	0.050210	0.022455
400 Gy	5	2.3953	0.4790	0.0014	0.038588	0.017257
ANOVA single factor (α = 0.01)
Source of variation	SS	df	MS	F	P-value	F crit.
Between groups	0.152096309	4	0.038024077	23.466281	0.0000003965	4.500257699
Within groups	0.030787046	19	0.001620371
Total	0.182883355	23

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of free radical scavenging potential of the mycelium extracts.

Table 4

The one-way ANOVA of total phenolic content of the mycelium extracts

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	439.4212	87.8842	98.0936	9.9042	4.4293
100 Gy	4	451.3970	112.8492	124.0126	11.1361	5.5680
200 Gy	5	632.8615	126.5723	76.2878	8.7343	3.9061
300 Gy	5	684.2167	136.8433	417.0659	20.4222	9.1331
400 Gy	5	667.4257	133.4851	238.70581	15.4501	6.9095
ANOVA single factor (α = 0.01)
Source of variation	SS	df	MS	F	P-value	F crit
Between groups	7 904.44478	4	1 976.111195	10.16779312	0.00014191	4.500257699
Within groups	3 692.651123	19	194.3500591
Total	11 597.0959	23

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of total phenolic content of the mycelium extracts.

Table 5

The one-way ANOVA of total phenolic content of the fermented broths

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	73.6308	14.72610	4.2755	2.0678	0.9247
100 Gy	5	72.1087	14.42170	0.4407	0.6639	0.2969
200 Gy	5	103.1155	20.62311	7.9304	2.8161	1.2594
300 Gy	5	111.8099	22.36199	3.9634	1.9908	0.8903
400 Gy	5	120.0085	24.00170	15.6380	3.9545	1.7685
ANOVA single factor (α = 0.01)
Source of variation	SS	df	MS	F	P-value	F crit.
Between groups	389.6157862	4	97.403946550	15.10220151	0.000007715	4.430690161
Within groups	128.9930431	20	6.449652157
Total	518.6088293	24

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of total phenolic content of the fermented broths.

Table 6

The one-way ANOVA of total flavonoid content of the fermented broths

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	0.5825	0.1165	0.00007	0.008539	0.003819
100 Gy	5	0.9134	0.1826	0.00974	0.098740	0.044158
200 Gy	5	5.7376	1.1475	0.26596	0.515721	0.230637
300 Gy	5	5.9381	1.1876	0.10480	0.323743	0.144782
400 Gy	5	6.0272	1.2054	0.18652	0.431886	0.193145
ANOVA single factor (α = 0.01)
Source of variation	SS	df	MS	F	P-value	F crit.
Between groups	6.392584426	4	1.598146107	14.08987153	0.000012734	4.430690161
Within groups	2.268503447	20	0.113425172
Total	8.661087873	24

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of total flavonoid content of the fermented broths.

Table 7

The one-way ANOVA of the sinapinic acid content of the fermentation broths

Groups	Count	Sum	Average	Variance	Std. deviation	Std. error
0 Gy	5	40.0005	8.0001	25.7390	5.073365	2.536682
100 Gy	3	51.3022	17.1007	26.4171	5.139765	3.634363
200 Gy	2	58.7856	29.3928	304.6469	20.619987	14.580533
300 Gy	3	91.1330	30.3776	18.1002	4.254438	3.008342
400 Gy	1	55.2323	55.2323	0	0	0
ANOVA single factor (α= 0.01)
Source of variation	SS	df	MS	F	P-value	F crit.
Between groups	2466.721606	4	616.6804015	11.17539311	0.001532718	6.422085458
Within groups	496.6378863	9	55.18198737
Total	2963.359492	13

[i] SS, sum of squares; df, degrees of freedom; MS, mean square.

The radiation dose-dependent distribution of sinapinic acid content of the fermentation broths.

Articles in this issue

DOI: https://doi.org/10.2478/nuka-2021-0012 | Journal eISSN: 1508-5791 (formerly 0029-5922) | Journal ISSN: 0029-5922

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Language: English

Page range: 83 - 90

Submitted on: Feb 2, 2021

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Accepted on: Jun 7, 2021

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Published on: Sep 17, 2021

Published by: Institute of Nuclear Chemistry and Technology

In partnership with: Paradigm Publishing Services

Keywords:

Gamma irradiation,

Medicinal fungi,

Metabolite synthesis,

Bioactive compounds

Related subjects:

Nuclear chemistry

© 2021 Alexandru Petre, Mihaela Ene, Daniel Constantin Negut, Florentina Gatea, Emanuel Vamanu, published by Institute of Nuclear Chemistry and Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 66 (2021): Issue 3 (September 2021)