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The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures Cover

The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures

Open Access
|Sep 2021

Figures & Tables

Fig. 1

The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the first experimental run.

Fig. 2

The radiation dose-dependent distribution of dry mycelium weight per 400 mL of culture, in the second experimental run.

Fig. 3

The radiation dose-dependent distribution of free radical scavenging potential of the mycelium extracts.

Fig. 4

The radiation dose-dependent distribution of total phenolic content of the mycelium extracts.

Fig. 5

The radiation dose-dependent distribution of total phenolic content of the fermented broths.

Fig. 6

The radiation dose-dependent distribution of total flavonoid content of the fermented broths.

Fig. 7

The radiation dose-dependent distribution of sinapinic acid content of the fermentation broths.

The one-way ANOVA of total phenolic content of the mycelium extracts

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy5439.421287.884298.09369.90424.4293
100 Gy4451.3970112.8492124.012611.13615.5680
200 Gy5632.8615126.572376.28788.73433.9061
300 Gy5684.2167136.8433417.065920.42229.1331
400 Gy5667.4257133.4851238.7058115.45016.9095

ANOVA single factor (α = 0.01)

Source of variationSSdfMSFP-valueF crit

Between groups7 904.4447841 976.11119510.167793120.000141914.500257699
Within groups3 692.65112319194.3500591
Total11 597.095923

The one-way ANOVA of total flavonoid content of the fermented broths

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy50.58250.11650.000070.0085390.003819
100 Gy50.91340.18260.009740.0987400.044158
200 Gy55.73761.14750.265960.5157210.230637
300 Gy55.93811.18760.104800.3237430.144782
400 Gy56.02721.20540.186520.4318860.193145

ANOVA single factor (α = 0.01)

Source of variationSSdfMSFP-valueF crit.

Between groups6.39258442641.59814610714.089871530.0000127344.430690161
Within groups2.268503447200.113425172
Total8.66108787324

The one-way ANOVA of free radical scavenging potential of the mycelium extracts

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy51.35620.27120.00190.0443820.019848
100 Gy41.64890.41220.00110.0341400.017070
200 Gy52.17220.43440.00080.0290300.012983
300 Gy52.43860.48770.00250.0502100.022455
400 Gy52.39530.47900.00140.0385880.017257

ANOVA single factor (α = 0.01)

Source of variationSSdfMSFP-valueF crit.

Between groups0.15209630940.03802407723.4662810.00000039654.500257699
Within groups0.030787046190.001620371
Total0.18288335523

The one-way ANOVA of total phenolic content of the fermented broths

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy573.630814.726104.27552.06780.9247
100 Gy572.108714.421700.44070.66390.2969
200 Gy5103.115520.623117.93042.81611.2594
300 Gy5111.809922.361993.96341.99080.8903
400 Gy5120.008524.0017015.63803.95451.7685

ANOVA single factor (α = 0.01)

Source of variationSSdfMSFP-valueF crit.

Between groups389.6157862497.40394655015.102201510.0000077154.430690161
Within groups128.9930431206.449652157
Total518.608829324

The one-way ANOVA of dry mycelium weight after irradiation and culture of the first experimental run

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy56.781.3560.004680.0684110.030594
100 Gy57.091.4180.003420.0584810.026153
200 Gy57.731.5460.007530.0867760.038807
300 Gy58.071.6140.008630.0928980.041545
400 Gy58.121.6240.004330.0658030.029428

ANOVA single factor (α = 0.01)

Source of variationSSdfMSFP-valueF crit.

Between groups0.28637640.07159412.520811470.0000291824.430690161
Within groups0.114360200.005718
Total0.40073624

The one-way ANOVA of the sinapinic acid content of the fermentation broths

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy540.00058.000125.73905.0733652.536682
100 Gy351.302217.100726.41715.1397653.634363
200 Gy258.785629.3928304.646920.61998714.580533
300 Gy391.133030.377618.10024.2544383.008342
400 Gy155.232355.2323000

ANOVA single factor (α= 0.01)

Source of variationSSdfMSFP-valueF crit.

Between groups2466.7216064616.680401511.175393110.0015327186.422085458
Within groups496.6378863955.18198737
Total2963.35949213

The one-way ANOVA of dry mycelium weight after irradiation and culture of the second experimental run

GroupsCountSumAverageVarianceStd. deviationStd. error
0 Gy59.201.8400.005350.0731440.032710
100 Gy59.991.9980.004520.0672310.030066
200 Gy510.492.0980.003520.0593300.026532
300 Gy510.732.1460.008430.0918150.041060
400 Gy510.352.0700.004900.070.031304

ANOVA single factor (α= 0.01)

Source of variationSSdfMSFP-valueF crit.

Between groups0.28401640.07100413.286676650.0000193032.866081402
Within groups0.106880200.0053440.10688
Total0.39089624
DOI: https://doi.org/10.2478/nuka-2021-0012 | Journal eISSN: 1508-5791 | Journal ISSN: 0029-5922
Language: English
Page range: 83 - 90
Submitted on: Feb 2, 2021
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Accepted on: Jun 7, 2021
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Published on: Sep 17, 2021
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2021 Alexandru Petre, Mihaela Ene, Daniel Constantin Negut, Florentina Gatea, Emanuel Vamanu, published by Institute of Nuclear Chemistry and Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.