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Eco-friendly high-rate formation of silver nanoparticles in agave inulin and its bactericidal effect against Escherichia coli Cover

Eco-friendly high-rate formation of silver nanoparticles in agave inulin and its bactericidal effect against Escherichia coli

Open Access
|Jan 2024

Figures & Tables

Fig. 1.

Experimental setup for the synthesis of AgNPs. Variables were (1) agave inulin; (2) AgNO3; (3) temperature; (4) pH
Experimental setup for the synthesis of AgNPs. Variables were (1) agave inulin; (2) AgNO3; (3) temperature; (4) pH

Fig. 2.

UV-Vis absorbance of nanoparticles obtained using different agave inulin concentrations
UV-Vis absorbance of nanoparticles obtained using different agave inulin concentrations

Fig. 3.

UV-Vis absorbance of nanoparticles obtained using different AgNO3 concentration
UV-Vis absorbance of nanoparticles obtained using different AgNO3 concentration

Fig. 4.

UV-Vis absorbance of nanoparticles obtained using different temperatures
UV-Vis absorbance of nanoparticles obtained using different temperatures

Fig. 5.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a temperature of 75°C
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a temperature of 75°C

Fig. 6.

UV-Vis absorbance of nanoparticles obtained using different pH values
UV-Vis absorbance of nanoparticles obtained using different pH values

Fig. 7.

(a) UV-Vis spectra of nanoparticles obtained with a pH of 12 using different solutions in water. (b) Line calibration of the maximum absorbance versus the agave inulin concentration (mg/mL)
(a) UV-Vis spectra of nanoparticles obtained with a pH of 12 using different solutions in water. (b) Line calibration of the maximum absorbance versus the agave inulin concentration (mg/mL)

Fig. 8.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 11
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 11

Fig. 9.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 10
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using pH 10

Fig. 10.

(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a pH of 12
(a) Size distribution, fitted with a Log-normal model (dotted line) from several micrographs (for instance b, c, and d) of AgNPs obtained using a pH of 12

Fig. 11.

Bactericidal effect. LB medium was used to determine the bactericidal effect. (1) Positive control: LB medium; (2) Negative control: LB medium and 100 μL E. coli strain; (3) LB medium, 100 μL E. coli strain, and 100 μL agave inulin; (4) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S1bac); (5) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S2bac); (6) LB medium, 100 μL E. Coli strain, and 100 μL agave inulin
Bactericidal effect. LB medium was used to determine the bactericidal effect. (1) Positive control: LB medium; (2) Negative control: LB medium and 100 μL E. coli strain; (3) LB medium, 100 μL E. coli strain, and 100 μL agave inulin; (4) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S1bac); (5) LB medium, 100 μL of E. coli strain, and 100 μL of AgNPs (S2bac); (6) LB medium, 100 μL E. Coli strain, and 100 μL agave inulin

Parameters and values used in the preparation of samples to study agave inulin’s effect on the synthesis of AgNPs

SampleABCD
S1A61236.5
S2A121236.5
S3A181236.5
S4A241236.5

Parameters and values used in the preparation of samples to study pH effect on the synthesis of AgNPs

SampleABCD
S1D2012312
S2D2012311
S3D2012310
S4D201239.3
S5D201238.3

Contents of the test tubes to study antibacterial activity

TubeLB (mL)E. coli (μL)Inulin (μL)Sample (μL)
15
25100
35100100
45100S1bac (100)
55100S2bac (100)
65100100

Parameters and values used in the preparation of samples to study the AgNO3 effect on the synthesis of AgNPs

SampleABCD
S1B201236.5
S2B203236.5
S3B205236.5

Parameters and values used in the preparation of samples to study temperature effect on the synthesis of AgNPs

SampleABCD
S1C201236.5
S2C201406.5
S3C201606.5
S4C201756.5
DOI: https://doi.org/10.2478/msp-2023-0034 | Journal eISSN: 2083-134X | Journal ISSN: 2083-1331
Language: English
Page range: 62 - 73
Submitted on: Jul 3, 2023
Accepted on: Nov 25, 2023
Published on: Jan 9, 2024
Published by: Wroclaw University of Science and Technology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2024 María Teresa Sánchez-Vieyra, Miguel Ojeda-Martínez, Eden Oceguera-Contreras, Sergio Yair Rodríguez-Preciado, Mariana Díaz-Zaragoza, Brenda Esmeralda Martínez-Zérega, José Luis González-Solís, David Omar Oseguera-Galindo, published by Wroclaw University of Science and Technology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.