Distribution of Porcine Circovirus 2 in Wild Boar in Estonia
By: Tõnu Järveots, Tiiu Saar, Toivo Suuroja and Piret Hussar
References
- Maity, H.K., Samanta, K., Deb, R., Gupta, V.K. (2023). Revisiting porcine circovirus infection: recent insights and its significance in the piggery sector. Vaccines. 11(8): 1308. https://doi.org/10.3390/vaccines11081308 PMid:37631876 PMCid:PMC10457769
- Gillespie, J., Opriessnig, T., Meng, X.J., Pelzer, K., Buechner-Maxwell, V. (2009). Porcine circovirus type 2 and porcine circovirus-associated disease. J Vet Intern Med. 23(6): 1151-1163. https://doi.org/10.1111/j.1939-1676.2009.0389.x PMid:19780932 PMCid:PMC7166794
- Tan, C.Y., Opaskornkul, K., Thanawongnuwech, R., Arshad, S.S., Hassan, L., Ooi, P.T. (2020). First molecular detection and complete sequence analysis of porcine circovirus type 3 (PCV3) in Peninsular Malaysia. PLoS One 15(7): e0235832. https://doi.org/10.1371/journal.pone.0235832 PMid:32706778 PMCid:PMC7380639
- Prinz, C., Stillfried, M., Neubert, L.K., Denner, J. (2019). Detection of PCV3 in German wild boars. Virol J. 16(1): 25. https://doi.org/10.1186/s12985-019-1133-9 PMid:30795772 PMCid:PMC6387533
- Franzo, G., Delwart, E., Fux, R., Hause, B., Su, S., Zhou, J.Y., Segales, J. (2020). Genotyping porcine circovirus 3 (PCV3) nowadays: Does it make sense? Viruses 12(3): 265. https://doi.org/10.3390/v12030265 PMid:32121102 PMCid:PMC7150946
- Harding, J., Clark, E. (1997). Recognizing and diagnosing postweaning multisystemic wasting syndrome (PMWS). JSHAP. 5, 201-203.
- Segales, J., Allan, G.M., Domingo, M. (2005). Porcine circovirus diseases. Anim Health Res Rev. 6(2): 119-142. https://doi.org/10.1079/AHR2005106 PMid:16583778
- Olvera, A., Cortey, M., Segales, J. (2007). Molecular evolution of porcine circovirus type 2 genomes: Phylogeny and clonality. Virology 357(2): 175-185. https://doi.org/10.1016/j.virol.2006.07.047 PMid:16963096
- Franzo, G., Segalés, J. (2018). Porcine circovirus 2 (PCV-2) genotype update and proposal of a new genotyping methodology. PLoS One 13(12): e0208585. https://doi.org/10.1371/journal.pone.0208585 PMid:30521609 PMCid:PMC6283538
- Wang, Y., Noll, L., Lu, N., Porter, E., Stoyt, C., Zheng, W., Liu, X., et al. (2020). Genetic diversity and prevalence of porcine circovirus type 3 (PCV3) and type 2 (PCV2) in the Midwest of the USA during 2016-2018. Transbound Emerg Dis. 67(3): 1284-1294. https://doi.org/10.1111/tbed.13467 PMid:31886622
- Fanelli, A., Pellegrini, F., Camero, M., Catella, C., Buonavoglia, D., Fusco, G., Martella, M., Lanave, G. (2022). Genetic diversity of porcine circovirus types 2 and 3 in wild boar in Italy. Animals (Basel). 12(8): 953. https://doi.org/10.3390/ani12080953 PMid:35454199 PMCid:PMC9031215
- Segales, J., Rosell, C., Domingo, M. (2004). Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. Vet Microbiol. 98(2): 137-149. https://doi.org/10.1016/j.vetmic.2003.10.006 PMid:14741126
- Segales, J., Domingo, M. (2002). Postweaning multisystemic wasting syndrome (PMWS) in pigs: A review. Vet Q. 24(3): 109-124. https://doi.org/10.1080/01652176.2002.9695132 PMid:12400999
- Opriessnig, T., Meng, X.J., Halbur, P.G. (2007). Porcine circovirus type 2-associated disease: Update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Investig. 19(6): 591-615. https://doi.org/10.1177/104063870701900601 PMid:17998548
- Hammer, R., Ritzmann, M., Palzer, A., Lang, C., Hammer, B., Pesch, S., Ladinig, A. (2012). Porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 infections in wild boar (Sus scrofa) in southwestern Germany. J Wildl Dis. 48(1): 87-94. https://doi.org/10.7589/0090-3558-48.1.87 PMid:22247377
- Kristensen, C.S., Hjulsager, C.K., Vestergaard, K., Dupont, K., Bille-Hansen, V., Enoe, C., Jorsal, S.E., Baekbo, P., Larsen, L.E. (2013). Experimental airborne transmission of porcine postweaning multisystemic wasting syndrome. J Pathog. 2013, 534342. https://doi.org/10.1155/2013/534342 PMid:23476787 PMCid:PMC3582107
- Rudova, N., Buttler, J., Kovalenko, G., Sushko, M., Bolotin, V., Muzykina, L., Zinenko, O., et al. (2022). Genetic diversity of porcine circovirus 2 in wild boar and domestic pigs in Ukraine. Viruses 14(5): 924. https://doi.org/10.3390/v14050924 PMid:35632666 PMCid:PMC9142977
- Sofia, M., Billinis, C., Psychas, V., Birtsas, P., Sofianidis, G., Leontides, L., Knowles, N., Spyrou, V.J. (2008). Detection and genetic characterization of porcine circovirus 2 isolates from the first cases of postweaning multisystemic and wasting syndrome in wild boars in Greece. J Wildl Dis. 44(4): 864-870. https://doi.org/10.7589/0090-3558-44.4.864 PMid:18957642
- Fabisiak, M., Szczotka, A., Podgórska, K., Stadejek, T. (2012). Prevalence of infection and genetic diversity of porcine circovirus type 2 (PCV2) in wild boar (Sus scrofa) in Poland. J Wildl Dis. 48(3): 612-618. https://doi.org/10.7589/0090-3558-48.3.612 PMid:22740527
- Amoroso, M.G., Serra, F., Esposito, C., D’Alessio, N., Ferrara, G., Cioffi, B., Anzalone, A., et al. (2021). Prevalence of infection with porcine circovirus types 2 and 3 in the wild boar population in the Campania region (southern Italy). Animals (Basel). 11(11): 3215. https://doi.org/10.3390/ani11113215 PMid:34827947 PMCid:PMC8614320
- Cadar, D., Cságola, A., Spinu, M., Dán, A., Ursu, K., Lorincz, M., Tuboly, T. (2010). Prevalence of porcine circoviruses in Transylvanian wild boars, detected by real-time PCR--short communication. Acta Vet Hung. 58(4): 475-481. https://doi.org/10.1556/avet.58.2010.4.8 PMid:21087917
- Krasnikov, N., Rykova, V., Kucheruk, O., Komina, A., Pchelnikov, A., Gulyukin, A., Yuzhakov, A. (2024). Genetic diversity of porcine circoviruses 2 and 3 circulating among wild boars in the Moscow Region of Russia. Front Vet Sci. 11, 1372203. https://doi.org/10.3389/fvets.2024.1372203 PMid:38988985 PMCid:PMC11233533
- Barbosa, C.N., Martins, N.R., Freitas, T.R., Lobato, Z.I. (2016). Serological survey of porcine circovirus-2 in captive wild boars (Sus scrofa) from registered farms of south and south-east regions of Brazil. Transbound Emerg Dis. 63(2): e278-e280. https://doi.org/10.1111/tbed.12247 PMid:25056836
- Klaumann, F., Dias-Alves, A., Cabezón, O., Mentaberre, G., Castillo-Contreras, R., López-Béjar, M., Casas-Díaz, E., et al. (2019). Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa). Transbound Emerg Dis. 66(1): 91-101. https://doi.org/10.1111/tbed.12988 PMid:30091190 PMCid:PMC6912250
- Faustini, G., Poletto, F., Baston, R., Tucciarone, C.M., Legnardi, M., Dal Maso, M., Genna, V., et al. (2024). D for dominant: porcine circovirus 2d (PCV-2d) prevalence over other genotypes in wild boars and higher viral flows from domestic pigs in Italy. Front Microbiol. 15, 1412615. https://doi.org/10.3389/fmicb.2024.1412615 PMid:38952451 PMCid:PMC11215180
- Nurmoja, I., Schulz, K., Staubach, C. (2017). Development of African swine fever epidemic among wild boar in Estonia - two different areas in the epidemiological focus. Sci Rep. 7(1): 12562. https://doi.org/10.1038/s41598-017-12952-w PMid:28970577 PMCid:PMC5624900
- Schulz, K., Staubach, C., Blome, S. (2019). Analysis of Estonian surveillance in wild boar suggests a decline in the incidence of African swine fever. Sci Rep. 9, 8490. https://doi.org/10.1038/s41598-019-44890-0 PMid:31186505 PMCid:PMC6560063
- Carson, F.L. (1997). Histotechnology: A self-instructional text, 2nd ed. (p. 304). Chicago, IL, USA: ASCP Press
- Li, W., Li, Y., Li, M., Zhang, H., Feng, Z., Xu, H., Li, C., et al. (2024). Development and application of a blocking ELISA based on a N protein monoclonal antibody for the antibody detection against porcine reproductive and respiratory syndrome virus 2. Int J Biol Macromol. 269(Pt 2): 131842. https://doi.org/10.1016/j.ijbiomac.2024.131842 PMid:38679249
- Shin, M.K., Yoon, S.H., Kim, M.H., Lyoo, Y.S., Suh, S.W., Yoo, H.S. (2015). Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems. J Vet Sci. 16(1): 25-29. https://doi.org/10.4142/jvs.2015.16.1.25 PMid:25234326 PMCid:PMC4367146
- Aydin, S., Emre, E., Ugur, K., Aydin, MA., Sahin, İ., Cinar, V., Akbulut, T. (2025). An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids. J Int Med Res. 53(2): 3000605251315913. https://doi.org/10.1177/03000605251315913 PMid:39922798 PMCid:PMC11808753
- Fablet, C., Rose, N., Bernard, C., Messager, I., Piel, Y., Grasland, B. (2017). Estimation of the diagnostic performance of two ELISAs to detect PCV2 antibodies in pig sera using a Bayesian method. J Virol Methods. 249, 121-125. https://doi.org/10.1016/j.jviromet.2017.09.002 PMid:28882762
- Malmsten, A., Magnusson, U., Ruiz-Fons, F., González-Barrio, D., Dalin, A.M. (2018). A serologic survey of pathogens in wild boar (Sus scrofa) in Sweden. J Wildl Dis. 54(2): 229-237. https://doi.org/10.7589/2017-05-120 PMid:29377751
- Ghebremariam, M.K., Gruys, E. (2005). PMWS in pigs with particular emphasis on the causative agent, the mode of the transmission, the diagnostic tools and the control measures. A review. Vet Q. 27(3): 105-116. https://doi.org/10.1080/01652176.2005.9695191 PMid:16238110
- Kroeger, M., Vargas-Bermudez, D.S., Jaime, J., Parada, J., Groeltz, J., Gauger, P., Piñeyro, P. (2024). First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues. Sci Rep. 14(1): 15535. https://doi.org/10.1038/s41598-024-66328-y PMid:38969759 PMCid:PMC11226432
- Borba, M.R., Sanches, E.M.C., Corrêa, A.M.R., Spanamberg, A., de Souza Leal, J., Soares, M.P., Guillot, J., Driemeier, D., Ferreiro, L. (2011). Immunohistochemical and ultra-structural detection of Pneumocystis in wild boars (Sus scrofa) co-infected with porcine circovirus type 2 (PCV2) in Southern Brazil. Med Mycol. 49(2): 172-175. https://doi.org/10.3109/13693786.2010.510540 PMid:20807029
- Morandi, F., Verin, R., Sarli, G., Canetti, N., Scacco, M., Panarese, S., Poli, A. (2010). Porcine circovirus type 2 (PCV2) antigen localisation and post-weaning multisystemic wasting syndrome (PMWS) in free-ranging wild boar (Sus scrofa ssp scrofa) in Italy. Eur J Wildl Res. 56(5): 717-724. https://doi.org/10.1007/s10344-010-0365-1
DOI: https://doi.org/10.2478/macvetrev-2026-0019 | Journal eISSN: 1857-7415
Language: English
Submitted on: Nov 7, 2025
Accepted on: Apr 28, 2026
Published on: Jul 12, 2026
Published by: Ss. Cyril and Methodius University in Skopje
In partnership with: Paradigm Publishing Services
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© 2026 Tõnu Järveots, Tiiu Saar, Toivo Suuroja, Piret Hussar, published by Ss. Cyril and Methodius University in Skopje
This work is licensed under the Creative Commons Attribution 4.0 License.