Evaluation of the Efficiency of Detection of Bacterial DNA in Milk and Tissue Samples from Cattle, Sheep, and Goats by Conventional and Nested PCR Targeting COM1, SOD and Transposase IS1111 Genes of Coxiella burnetii Genome
References
- Madariaga, M.G., Rezai, K., Trenholme, G.M., Weinstein, R.A. (2003). Q fever: a biological weapon in your backyard. Lancet Infect Dis. 3(11): 709-721. https://doi.org/10.1016/S1473-3099(03)00804-1 PMid:14592601
- Angelakis, E., Raoult, D. (2010). Q fever - review. Vet Microbiol. 140(3-4): 297-309. https://doi.org/10.1016/j.vetmic.2009.07.016 PMid:19875249
- Maurin, M., Raoult, D. (1999). Q fever. Clin Microbiol Rev. 12(4): 518-553. https://doi.org/10.1128/CMR.12.4.518 PMid:10515901 PMCid:PMC88923
- Melenotte, C., Protopopescu, C., Million, M., Edouard, S., Carrieri, M.P., Eldin, C., Angelakis, E., et al. (2018). Clinical features and complications of Coxiella burnetii infections from the French National Reference Center for Q fever. JAMA Network Open. 1(4): e181580. https://doi.org/10.1001/jamanetworkopen.2018.1580 PMid:30646123 PMCid:PMC6324270
- Eldin, C., Mélenotte, C., Mediannikov, O., Ghigo, E., Million, M., Edouard, S., Mege, J.L., et al. (2017). From Q Fever to Coxiella burnetii infection: a paradigm change. Clin Microbiol Rev. 30(1): 115-190. https://doi.org/10.1128/CMR.00045-16 PMid:27856520 PMCid:PMC5217791
- Agerholm, J.S. (2013). Coxiella burnetii associated reproductive disorders in domestic animals -a critical review. Acta Vet Scand. 55(1): 13. https://doi.org/10.1186/1751-0147-55-13 PMid:23419216 PMCid:PMC3577508
- Astobiza, I., Barandika, J.F., Ruiz-Fons, F., Hurtado, A., Povedano, I., Juste, R.A., García-Pérez, A.L. (2011). Coxiella burnetii shedding and environmental contamination at lambing in two highly naturally-infected dair y sheep f locks after vaccination. Res Vet Sci. 91(3): e58-63. https://doi.org/10.1016/j.rvsc.2010.11.014 PMid:21168178
- Arricau-Bouvery, N., Souriau, A., Lechopier, P., Rodolakis, A. (2003). Experimental Coxiella burnetii infection in pregnant goats: Excretion routes. Vet Res. 34(4): 423-433. https://doi.org/10.1051/vetres:2003017 PMid:12911859
- Berri, M., Laroucau, K., Rodolakis, A. (2000). The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Vet Microbiol. 72(3-4): 285-293. https://doi.org/10.1016/S0378-1135(99)00178-9 PMid:10727838
- Fournier, P.E., Raoult, D. (2003). Comparison of PCR and serology assays for early diagnosis of acute Q fever. J Clin Microbiol. 41(11): 5094-5098. https://doi.org/10.1128/JCM.41.11.5094-5098.2003 PMid:14605144 PMCid:PMC262519
- Van den Brom, R., van Engelen, E., Roest, H.I., van der Hoek, W., Vellema, P. (2015). Coxiella burnetii infections in sheep or goats: an opinionated review. Vet Microbiol. 181 (1-2): 119-129. https://doi.org/10.1016/j.vetmic.2015.07.011 PMid:26315774
- Sahu, R., Rawool, D.B., Vinod, V.K., Malik, S.V.S., Barbuddhe, S.B. (2020). Current approaches for the detection of Coxiella burnetii infection in humans and animals. J Microbiol Methods. 179, 106087. https://doi.org/10.1016/j.mimet.2020.106087 PMid:33086105
- Zhang, G.Q., Hotta, A., Mizutani, M., Ho, T., Yamaguchi, T., Fukushi, H., Hirai, K. (1998). Direct identification of Coxiella burnetii plasmids in human sera by nested PCR. J Clin Microbiol. 36(8): 2210-2213. https://doi.org/10.1128/JCM.36.8.2210-2213.1998 PMid:9665993 PMCid:PMC105014
- Stein, A., Raoult, D. (1992). Detection of Coxiella burnetti by DNA amplification using polymerase chain reaction. J Clin Microbiol. 30(9): 2462-2466. https://doi.org/10.1128/jcm.30.9.2462-2466.1992 PMid:1401016 PMCid:PMC265524
- Zhang, G.Q., Nguyen, S.V., To, H., Ogawa, M., Hotta, A., Yamaguchi, T., Kim, H.J., et al. (1998). Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples. J Clin Microbiol. 36(1): 77-80. https://doi.org/10.1128/JCM.36.1.77-80.1998 PMid:9431924 PMCid:PMC124811
- Berri, M., Arricau-Bouvery, N., Rodolakis, A. (2003). PCR-based detection of Coxiella burnetii from clinical samples. Methods Mol Biol. 216, 153-161. https://doi.org/10.1385/1-59259-344-5:153 PMID: 12512362
- Hoover, T.A., Vodkin, M.H., Williams, J.C. (1992). A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence. J Bacteriol. 174(17): 5540-5548. https://doi.org/10.1128/jb.174.17.5540-5548.1992 PMid:1324903 PMCid:PMC206497
- Klee, S.R., Ellerbrok, H., Tyczka, J., Franz, T., Appel, B. (2006). Evaluation of a real-time PCR assay to detect Coxiella burnetii. Ann N Y Acad Sci. 1078, 563-565. https://doi.org/10.1196/annals.1374.111 PMid:17114778
- Denison, A.M., Thompson, H.A., Massung, R.F. (2007). IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates. BMC Microbiol. 7, 91. https://doi.org/10.1186/1471-2180-7-91 PMid:17949485 PMCid:PMC2104537
- de Bruin, A., de Groot, A., de Heer, L., Bok, J., Wielinga, P.R., Hamans, M., van Rotterdam, B.J., Janse, I. (2011). Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands. Appl Environ Microbiol. 77(18): 6516-6523. https://doi.org/10.1128/AEM.05097-11 PMid:21784920 PMCid:PMC3187144
- Marmion, B.P., Storm, P.A., Ayres, J.G., Semendric, L., Mathews, L., Winslow, W., Turra, M., Harris, R.J. (2005). Long-term persistence of Coxiella burnetii after acute primary Q fever. QJM 98(1): 7-20. https://doi.org/10.1093/qjmed/hci009 PMid:15625349
- Kargar, M., Rashidi, A., Doosti, A., Najafi, A., Ghorbani-Dalini, S. (2015). The sensitivity of the PCR method for detection of Coxiella burnetii in the milk samples. ZJRMS 17(6): e988. https://doi.org/10.17795/zjrms988
- Basanisi, M.G., La Bella, G., Nobili, G., Raele, D.A., Cafiero, M.A., Coppola, R., Damato, A.M., et al. (2022). Detection of Coxiella burnetii DNA in sheep and goat milk and dairy products by droplet digital PCR in south Italy. Int J Food Microbiol. 366, 109583. https://doi.org/10.1016/j.ijfoodmicro.2022.109583 PMid:35182931
- Edouard, S., Raoult, D. (2016). Lyophilization to improve the sensitivity of qPCR for bacterial DNA detection in serum: the Q fever paradigm. J Med Microbiol. 65(6): 462-467. https://doi.org/10.1099/jmm.0.000253 PMid:27008653
- Jones, R.M., Twomey, D.F., Hannon, S., Errington, J., Pritchard, G.C., Sawyer, J. (2010). Detection of Coxiella burnetii in placenta and abortion samples from British r uminants using real-time PCR. Vet Rec. 167(25): 965-967. https://doi.org/10.1136/vr.c4040 PMid:21262712
- Ogawa, M., Setiyono, A., Sato, K., Cai, Y., Shiga, S., Kishimoto, T. (2004). Evaluation of PCR and nested PCR assays currently used for detection of Coxiella burnetii in Japan. Southeast Asian J Trop Med Public Health. 35(4): 852-855.
- Mares-Guia, M.A.M.M., Guterres, A., Rozental, T., Ferreira, M.D.S., Lemos, E.R.S. (2018). Clinical and epidemiological use of nested PCR targeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii. Braz J Microbiol. 49(1): 138-143. https://doi.org/10.1016/j.bjm.2017.04.009 PMid:28899604 PMCid:PMC5790644
- Abiri, Z., Khalili, M., Kostoulas, P., Sharifi, H., Rad, M., Babaei, H. (2019). Bayesian estimation of sensitivity and specificity of a PCR method to detect Coxiella burnetii in milk and vaginal secretions in sheep and goat samples. J Dairy Sci. 102(6): 4954-4959. https://doi.org/10.3168/jds.2018-15233 PMid:31005328
- Klee, S.R., Tyczka, J., Ellerbrok, H., Franz, T., Linke, S., Baljer, G., Appel, B. (2006). Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol. 6, 2. https://doi.org/10.1186/1471-2180-6-2 PMid:16423303 PMCid:PMC1360083
- Rolain, J.M., Raoult, D. (2005). Molecular detection of Coxiella burnetii in blood and sera during Q fever. QJM 98(8): 615-617. https://doi.org/10.1093/qjmed/hci099 PMid:16027172
- Gardner, B., Bachmann, N., Polkinghorne, A., Hufschmid, J., Tadepalli, M., Marenda, M., Graves, S., et al. (2023). Novel marine mammal Coxiella burnetii-genome sequencing identifies a new genotype with potential virulence. Pathogens. 12(7): 893. https://doi.org/10.3390/pathogens12070893 PMid:37513739 PMCid:PMC10386718
- Huggett, J.F., Novak, T., Garson, J.A., Green, C., Morris-Jones, S.D., Miller, R.F., Zumla, A. (2008). Differential susceptibility of PCR reactions to inhibitors: an impor tant and unrecognized phenomenon. BMC Res Notes. 1, 70. https://doi.org/10.1186/1756-0500-1-70 PMid:18755023 PMCid:PMC2564953
DOI: https://doi.org/10.2478/macvetrev-2025-0020 | Journal eISSN: 1857-7415
Language: English
Page range: 137 - 146
Submitted on: Jun 21, 2024
Accepted on: Feb 28, 2025
Published on: Apr 16, 2025
Published by: Ss. Cyril and Methodius University in Skopje
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Related subjects:
© 2025 Konstantin Borisov Simeonov, Keytlin Venelinova Todorova, Petia Dinkova Genova-Kalou, published by Ss. Cyril and Methodius University in Skopje
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.