Breed-related and stage-related differences in the immune response to Staphylococcus aureus carriage in sheep during the periparturient period
References
- Abdelmegid S., Murugaiyan J., Abo-Ismail M., Caswell J.L., Kelton D., Kirby G.M.: Identification of host defense-related proteins using label-free quantitative proteomic analysis of milk whey from cows with Staphylococcus aureus subclinical mastitis. Int J Mol Sci 2017, 19, 78, doi: 10.3390/ijms19010078.
- Bonnefont C.M., Rainard P., Cunha P., Gilbert F.B., Toufeer M., Aurel M.R., Rupp R., Foucras G.: Genetic susceptibility to S. aureus mastitis in sheep: differential expression of mammary epithelial cells in response to live bacteria or supernatant. Physiol Genomics 2012, 44, 403–416, doi: 10.1152/physiolgenomics.00155.2011.
- Bröker B.M., Mrochen D., Péton V.: The T Cell response to Staphylococcus aureus. Pathogens 2016, 5, 31, doi: 10.3390/pathogens5010031.
- Garba B., Habibullah S.A., Saidu B., Suleiman N.: Effect of mastitis on some hematological and biochemical parameters of Red Sokoto goats. Vet World 2019, 12, 572–577, doi: 10.14202/vetworld.2019.572-577.
- Gilbert F.B., Cunha P., Jensen K., Glass E.J., Foucras G., Robert-Granié C., Rupp R., Rainard P.: Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 2013, 44, 40, doi: 10.1186/1297-9716-44-40.
- Greguła-Kania M., Kosior-Korzecka U., Patkowski K., Juszczuk-Kubiak E., Plewik M., Gruszecki T.M.: Acute-phase proteins, cortisol and haematological parameters in ewes during the periparturient period. Reprod Domest Anim 2020, 55, 393–400, doi: 10.1111/rda.13628.
- Greguła-Kania M., Patkowski K., Kosior-Korzecka U., Gruszecki T.M.: Reference gene selection for quantitative Real-Time PCR analysis in ovine whole blood. International Conference Animal Genetics and Genomics 2018, Book of abstracts (89–90).
- Hadfield J.M., Bowdridge E.C., Holásková I., Elsasser T.H., Dailey R.A.: Breed-specific differences in the immune response to lipopolysaccharide in ewes. J Anim Sci 2018, 96, 4220–4228, doi: 10.1093/jas/sky288.
- Hahaj-Siembida A., Nowakiewicz A., Bochniarz M., Trościańczyk A., Osińska M., Tracz A., Junkuszew A., Savvulidi Vargova K., Greguła-Kania M.: Perinatal occurrence and epidemiological significance of Staphylococcus aureus in local sheep breeds. Animals 2026, 16, 400, doi: 10.3390/ani16030400.
- Hahaj-Siembida A., Nowakiewicz A., Greguła-Kania M., Bochniarz M., Trościańczyk A., Osińska M.: Characteristics of drug-resistant staphylococci isolated from milk of lambed ewes during the perinatal period. J Vet Res 2025, 69, 41–50, doi: 10.2478/jvetres-2025-0014.
- Hahaj-Siembida A., Nowakiewicz A., Korzeniowska-Kowal A., Szecówka K., Trościańczyk A., Zięba P., Greguła-Kania M.: Red foxes (Vulpes vulpes) as a specific and underappreciated reservoir of resistant and virulent coagulase-positive Staphylococcus spp. strains. Res Vet Sci 2024, 166, 105111, doi: 10.1016/j.rvsc.2023.105111.
- Jeżak K., Kozajda A.: Occurrence and spread of antibiotic-resistant bacteria on animal farms and in their vicinity in Poland and Ukraine-review. Environ Sci Pollut Res Int 2021, 29, 9533–9559, doi: 10.1007/s11356-021-17773-z.
- Kamińska P., Tempes A., Scholz E., Malik A.R.: Cytokines on the way to secretion. Cytokine Growth Factor Rev 2024, 79, 52–65, doi: 10.1016/j.cytogfr.2024.08.003.
- Kawecka-Grochocka E., Zalewska M., Rzewuska M., Kościuczuk E., Ząbek T., Sakowski T., Marczak S., Bagnicka E.: Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection. Vet Res 2021, 52, 132, doi: 10.1186/s13567-021-01003-y.
- Kotzamanidis C., Vafeas G., Giantzi V., Anastasiadou S., Mygdalias S., Malousi A., Loukia E., Daniel S., Zdragas A.: Staphylococcus aureus isolated from ruminants with mastitis in northern Greece dairy herds: genetic relatedness and phenotypic and genotypic characterization. Toxins 2021, 13, 176, doi: 10.3390/toxins13030176.
- Latimer K.S.: Duncan & Prasse’s Veterinary Laboratory Medicine: Clinical Pathology, fifth edtion, Wiley-Blackwell, Ames, IA, USA, 2011.
- Mauffré V., Grimard B., Eozenou C., Inghels S., Silva L., Giraud-Delville C., Capo D., Sandra O., Constant F.: Interferon stimulated genes as peripheral diagnostic markers of early pregnancy in sheep: a critical assessment. Animal 2016, 10, 1856–1863, doi: 10.1017/S175173111600077X.
- Merz A., Stephan R., Johler S.: Staphylococcus aureus isolates from goat and sheep milk seem to be closely related and differ from isolates detected from bovine milk. Front Microbiol 2016, 7, 319, doi: 10.3389/fmicb.2016.00319.
- Mikkelsen J.H., Runager K., Andersen C.B.F.: The human protein haptoglobin inhibits IsdH-mediated heme-sequestering by Staphylococcus aureus. J Biol Chem 2020, 295, 1781–1791, doi: 10.1074/jbc.RA119.011612.
- Mills K.H.G.: IL-17 and IL-17-producing cells in protection versus pathology. Nat Rev Immunol 2023, 23, 38–54, doi: 10.1038/s41577-022-00746-9.
- Petersen H.H., Nielsen J.P., Heegaard P.M.: Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 2004, 35, 163–187, doi: 10.1051/vetres:2004002.
- Pfaffl M.W.: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001, 29, e45, doi: 10.1093/nar/29.9.e45.
- Radocchia G., Brunetti F., Marazzato M., Totino V., Neroni B., Bonfiglio G., Conte A.L., Pantanella F., Ciolli P., Schippa S.: Women skin microbiota modifications during pregnancy. Microorganisms 2024, 12, 808, doi: 10.3390/microorganisms12040808.
- Rahman M.M., Lecchi C., Fraquelli C., Sartorelli P., Ceciliani F.: Acute phase protein response in Alpine ibex with sarcoptic mange. Vet Parasitol 2010, 168, 293–298, doi: 10.1016/j.vetpar.2009.12.001.
- Renna M.S., Silvestrini P., Beccaria C., Velázquez N.S., Baravalle C., Engler C., Pereyra E.A.L., Calvinho L.F., Dallard B.E.: Effects of chronic Staphylococcus aureus infection on immunological parameters and functionality of macrophages isolated from bovine mammary secretions. Microb Pathog 2019, 137, 103743, doi: 10.1016/j.micpath.2019.103743.
- Rossi B.F., Bonsaglia É.C.R., Castilho I.G., Dantas S.T.A., Langoni H., Pantoja J.C.F., Júnior A.F., Gonçalves J.L., Santos M.V., Mota R.A., Rall V.L.M.: First investigation of Staphylococcus argenteus in a Brazilian collections of S. aureus isolated from bovine mastitis. BMC Vet Res 2020, 16, 252, doi: 10.1186/s12917-020-02472-7.
- Sordillo E.M., Polsky B.: Chapter 47, Infections in Pregnancy. In: Principles of Gender-Specific Medicine, second edition, edited by M.J. Legato, Academic Press, Cambridge, MA, USA, 2010, pp 531–562, doi: 10.1016/B978-0-12-374271-1.00047-2.
- Thompson-Crispi K., Atalla H., Miglior F., Mallard B.A.: Bovine mastitis: frontiers in immunogenetics. Front Immunol 2014, 5, 493, doi: 10.3389/fimmu.2014.00493.
- Vautor E., Cockfield J., Le Marechal C., Le Loir Y., Chevalier M., Robinson D.A., Thiery R, Lindsay J.: Difference in virulence between Staphylococcus aureus isolates causing gangrenous mastitis versus subclinical mastitis in a dairy sheep flock. Vet Res 2009, 40, 56, doi: 10.1051/vetres/2009039.
- Weiss DJ, Wardrop KJ, Schalm’s Veterinary Hematology, sixth edition, Wiley-Blackwell, Ames, IA, USA, 2010.
- Zhang J.M., An J.: Cytokines, inflammation, and pain. Int Anesthesiol Clin 2007, 45, 27–37, doi: 10.1097/AIA.0b013e318034194e.
DOI: https://doi.org/10.2478/jvetres-2026-0043 | Journal eISSN: 2450-8608 (formerly 2300-3235)
Language: English
Submitted on: Feb 4, 2026
Accepted on: Aug 3, 2026
Published on: Aug 7, 2026
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Keywords:
Related subjects:
© 2026 Agata Hahaj-Siembida, Aneta Nowakiewicz, Mariola Bochniarz, Aleksandra Trościańczyk, Marcelina Osińska, Andrzej Junkuszew, Karina Savvulidi Vargova, Monika Gregula-Kania, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.