Fig. 1.
Sampling time points and types of samples collected from turkeys for Mycoplasma infection testing
Table 1.
Results of ELISA tests for Mycoplasma meleagridis in a breeding turkey flock in the rearing period
| Poultry house | Week 22 | Week 24 | Week 26 |
|---|---|---|---|
| H1 | negative (23/23; 100%) | negative (23/23; 100%) | negative (23/23; 100%) |
| H2 | negative (23/23; 100%) | negative (23/23; 100%) | negative (23/23; 100%) |
| H3 | positive (1/23; 4%) | positive (1/23; 4%) | positive (2/23; 9%) |
Table 2.
Results of real-time PCR to amplify Mycoplasma DNA in samples from a breeding turkey flock in the rearing period
| Poultry house | Week 24 Mycoplasma meleagridis | Week 24 Mycoplasma spp. | Week 26 Mycoplasma meleagridis | Week 26 Mycoplasma spp. |
|---|---|---|---|---|
| H1 | negative (3/3; 100%) | negative (3/3; 100%) | negative (3/3; 100%) | negative (3/3; 100%) |
| H2 | negative (3/3; 100%) | negative (3/3; 100%) | negative (3/3; 100%) | negative (3/3; 100%) |
| H3 | negative (6/6; 100%) | positive/inconclusive (6/6; 100%) (Ct 35.1; 35.3; 37; 37.3; 38; 38.7) | negative (3/3; 100%) | inconclusive (1/6; 17%) (Ct 39) |
Table 3.
Results of real-time PCR to amplify Mycoplasma DNA in samples from a breeding turkey flock in the laying period
| Poultry house | Mycoplasma spp. in cloacal swabs | Mycoplasma spp. in tracheal swabs | Mycoplasma meleagridis in cloacal swabs | Mycoplasma meleagridis in tracheal swabs |
|---|---|---|---|---|
| H1 | positive (3/5; 60%) (Ct 34.6, 36.1, 36.3) | positive/inconclusive (3/5; 60%) (Ct 34.2, 37.7, 38.2) | negative (5/5; 100%) | negative (5/5; 100%) |
| H2 | positive (4/5; 80%) (Ct 32.1, 33.9, 34.1, 35.5) | positive/inconclusive (4/5; 80%) (Ct 36.7, 36.8, 37.1, 38.0) | negative (5/5; 100%) | negative (5/5; 100%) |
| H3 | positive (4/5; 80%) (Ct 29.6, 33.9, 36,5, 36.8) | negative (5/5; 100%) | negative (5/5; 100%) | negative (5/5; 100%) |
| H4 | positive/inconclusive (3/5; 60%) (Ct 36.0, 37.1, 37.9) | negative (5/5; 100%) | negative (5/5; 100%) | negative (5/5; 100%) |
Table 4.
Results of Sanger sequencing and BLAST analysis of Mycoplasma gallinarum isolates from breeding turkey flocks in the rearing and laying periods
| Flock period | Poultry house | Species identified | Sequence identity (%) | Query coverage (%) | Closest GenBank accession No. |
|---|---|---|---|---|---|
| Rearing | H3 | Mycoplasma gallinarum | 99.19 | 100 | MH538998.1 |
| Laying | H1 | Mycoplasma gallinarum | 99.08 | 100 | MH538998.1 |
| Laying | H3 | Mycoplasma gallinarum | 99.08 | 100 | JN935884.1 |