Fig 1.
The mean serum hormone concentrations in the vaginal lavage samples of primiparous and multiparous Holstein dairy cows
Table 1.
PCR mixture constituents for cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora analyses
| LongAmp Taq 2x Master Mix | 25 μL |
|---|---|
| Barcode Primer | 10 μL |
| double-distilled H2O | 5 μL |
| Template DNA | 10 μL |
| Total volume | 50 μL |
Table 2.
PCR thermal cycling for cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora analyses
| Temperature | Duration | Number of cycles |
|---|---|---|
| 95°C | 1 min | 1 |
| 95°C | 20 s | |
| 55°C | 30 s | 25 |
| 65°C | 2 min | |
| 65°C | 5 min | 1 |
| 4°C | ∞ | 1 |
Fig. 2.
Mean phylum occurrences in cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora
Fig. 3A
Proportions of the phyla in the groups
Fig. 3B
Proportions of the phyla in the follicular cyst group
Fig. 3C
Proportions of the phyla in the luteal cyst group
Fig. 3D.
Proportions of the phyla in the control group
Fig. 4.
Mean bacterial family occurrence in cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora
Fig. 5.
Mean bacterial genus occurrence in cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora
Fig. 6.
Proportions of the detected genera in cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora
Fig.7.
Beta diversities of cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora shown through weighted UniFrac distances and principal component analysis (PCoA). PC1/2 – principal component 1/2