Fig 1.
Fig. 2.
Fig. 3A
Fig. 3B
Fig. 3C
Fig. 3D.
Fig. 4.
Fig. 5.
Fig. 6.
Fig.7.
PCR thermal cycling for cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora analyses
| Temperature | Duration | Number of cycles |
|---|---|---|
| 95°C | 1 min | 1 |
| 95°C | 20 s | |
| 55°C | 30 s | 25 |
| 65°C | 2 min | |
| 65°C | 5 min | 1 |
| 4°C | ∞ | 1 |
PCR mixture constituents for cystic and healthy primiparous and multiparous Holstein dairy cow vaginal microflora analyses
| LongAmp Taq 2x Master Mix | 25 μL |
|---|---|
| Barcode Primer | 10 μL |
| double-distilled H2O | 5 μL |
| Template DNA | 10 μL |
| Total volume | 50 μL |