Fig. 1.
Sensitivity of the Flashtest multiplex qPCR assay for p72 (b646L) CD2v (ep402R) and an MGF targets
| Target | Number of TP | Number of FN | Sensitivity (95% CI) |
|---|---|---|---|
| p72 | 29 | 3* | 90.6% (75.8–96.8) |
| CD2v | 31 | 1* | 97.0% (84.7–99.5) |
| MGF | 29 | 3* | 90.6% (75.8–96.8) |
Reproducibility parameters Flashtest multiplex qPCR assay
| Target | Range (TCID50/mL) | Mean Ct(±SD) | CV (%) |
|---|---|---|---|
| p72 | High (106) | 20.97 (0.56) | 2.65 |
| Mid (104) | 27.76 (0.54) | 1.96 | |
| Low (103) | 32.02 (1.07) | 3.34 | |
| CD2v | High (106) | 20.09 (0.39) | 1.96 |
| Mid (104) | 26.76 (0.40) | 1.50 | |
| Low (103) | 30.88 (1.02) | 3.30 | |
| MGF | High (106) | 19.96 (0.30) | 1.51 |
| Mid (104) | 26.81 (0.38) | 1.41 | |
| Low (103) | 30.86 (0.82) | 2.67 |
Paired analysis of threshold cycle (Ct) values for the p72 (b646L) CD2v (ep402R) and an MGF targets, amplified using the Flashtest (Flash) assay in comparison with the Virotype (Viro) qPCR assay
| Target | Median difference in Ct (Flash/Viro) | Wilcoxon p | Spearman rs |
|---|---|---|---|
| p72 | 0.58 | 0.0506 | 0.9522 |
| CD2v | –0.24 | 0.8962 | 0.9633 |
| MGF | –0.54 | 0.6352 | 0.9527 |