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Evaluation of the Flashtest multiplex qPCR system for rapid pre-laboratory screening for African swine fever infection Cover

Evaluation of the Flashtest multiplex qPCR system for rapid pre-laboratory screening for African swine fever infection

Journal of Veterinary Research
Volume 70 (2026): Issue 2 (June 2026)
By:  and    
Open Access
|Apr 2026
Figures & tables

Figures & Tables

Table 1.

Sensitivity of the Flashtest multiplex qPCR assay for p72 (b646L) CD2v (ep402R) and an MGF targets

TargetNumber of TPNumber of FNSensitivity (95% CI)
p72293*90.6% (75.8–96.8)
CD2v311*97.0% (84.7–99.5)
MGF293*90.6% (75.8–96.8)

1 95% CI – 95% confidence interval; TP – true positives; FN – false negatives;

1* – samples below estimated limit of detection

Table 2.

Paired analysis of threshold cycle (Ct) values for the p72 (b646L) CD2v (ep402R) and an MGF targets, amplified using the Flashtest (Flash) assay in comparison with the Virotype (Viro) qPCR assay

TargetMedian difference in Ct (Flash/Viro)Wilcoxon pSpearman rs
p720.580.05060.9522
CD2v–0.240.89620.9633
MGF–0.540.63520.9527
Table 3.

Reproducibility parameters Flashtest multiplex qPCR assay

TargetRange (TCID50/mL)Mean Ct(±SD)CV (%)
p72High (106)20.97 (0.56)2.65
Mid (104)27.76 (0.54)1.96
Low (103)32.02 (1.07)3.34
CD2vHigh (106)20.09 (0.39)1.96
Mid (104)26.76 (0.40)1.50
Low (103)30.88 (1.02)3.30
MGFHigh (106)19.96 (0.30)1.51
Mid (104)26.81 (0.38)1.41
Low (103)30.86 (0.82)2.67

1 TCID50 –50% tissue culture infective dose; Ct – threshold cycle; CV – coefficient of variation

graphic/j_jvetres-2026-0022_fig_001.jpg
Fig. 1.

Linearity parameters of the Flashtest multiplex qPCR assay. Ct – threshold cycle; TCID50 – 50% tissue culture infective dose. Error bars indicate SD

DOI: https://doi.org/10.2478/jvetres-2026-0022 | Journal eISSN: 2450-8608 (formerly 2300-3235)
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Language: English
Page range: 185 - 190
Submitted on: Oct 6, 2025
Accepted on: Apr 10, 2026
Published on: Apr 16, 2026
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Keywords:
ASF,
ASFV,
qPCR,
diagnostics
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2026 Marek Walczak, Łukasz Adaszek, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 70 (2026): Issue 2 (June 2026)
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