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Use of ELISA to predict high Mycobacterium avium subsp. paratuberculosis shedding in faeces of infected animals Cover

Use of ELISA to predict high Mycobacterium avium subsp. paratuberculosis shedding in faeces of infected animals

Open Access
|Mar 2026

Figures & Tables

Fig. 1.

ELISA serum-to-positive (S/P) values of serum samples. (a) Animals grouped according to their qPCR crossing point (Cp) values, representing moderate-to-high (Cp < 31) and low (Cp ≥ 31) Mycobacterium avium subsp. paratuberculosis (MAP) faecal shedding. (b) Animals grouped according to their CFU count, representing high (CFU ≥ 100) and low (CFU < 100) MAP faecal shedding. Red dots and red line segments within the box plots indicate the group means of the S/P values and the 95% confidence intervals of these means

Fig. 2.

Faecal sample crossing point (Cp) values from qPCR. Samples grouped according to their CFU count, representing high (CFU ≥ 100) and low (CFU < 100) Mycobacterium avium subsp. paratuberculosis faecal shedding. Red dots and red line segments within the box plots indicate the group means of the Cp values and the 95% confidence intervals of these means

Results of Student’s t-test for the significance of differences between sample-to-positive ratios (S/P) in ELISA among animals with moderate-to-high (qPCR crossing point (Cp < 31) and low (Cp ≥ 31) Mycobacterium avium subsp_ paratuberculosis faecal shedding

Cp > 27Cp < 27P-value
Mean S/P2.142.390.6025
Number of animals10715
Cp ≥ 31Cp < 31
Mean S/P2.062.470.0125
Number of animals9032

Results of Mann–Whitney U test for the significance of differences between sample-to-positive ratios (S/P) in ELISA and crossing points (Cp) from qPCR of samples with lower (CFU < 100) and higher (CFU ≥ 100) Mycobacterium avium subsp_ paratuberculosis faecal shedding

CFU < 100CFU ≥ 100P-value
Mean S/P1.982.50.0215
Mean Cp35.6828.27<0.0001
Number of animals5627
Language: English
Page range: 169 - 176
Submitted on: Jun 18, 2025
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Accepted on: Mar 30, 2026
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Published on: Mar 31, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 Věra Fichtelová, Alena Králová, Vladimír Babák, Kamil Kovařčík, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.