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Relationship between kisspeptin-10, neurokinin B and dynorphin A in the course of normal and delayed puberty in ewes Cover

Relationship between kisspeptin-10, neurokinin B and dynorphin A in the course of normal and delayed puberty in ewes

Open Access
|Mar 2026

Figures & Tables

Fig. 1.

Concentration of kisspeptin-10 (KiSS-10) (ng/L) by month of life of singleton (S – n = 13) and twin (T – n = 11) ewes. The values presented in the figure are mean (±SD) concentrations in the plasma collected monthly every 15 min for 2 h. * – statistically significant difference between S and T (P-value ≤ 0.05); a, b, c, d and e – mean values in a particular month marked with different letters differ significantly (P-value ≤ 0.05)
Concentration of kisspeptin-10 (KiSS-10) (ng/L) by month of life of singleton (S – n = 13) and twin (T – n = 11) ewes. The values presented in the figure are mean (±SD) concentrations in the plasma collected monthly every 15 min for 2 h. * – statistically significant difference between S and T (P-value ≤ 0.05); a, b, c, d and e – mean values in a particular month marked with different letters differ significantly (P-value ≤ 0.05)

Fig. 2.

Concentration of neurokinin B (NKB) (ng/L) by month of life of singleton (S – n = 13) and twin (T – n = 11) ewes. The values presented in the figure are mean (±SD) concentrations in plasma collected monthly every 15 min for 2 h. * – statistically significant difference between S and T (P-value ≤ 0.05); a, b, c – mean values in a particular month marked with different letters differ significantly (P-value ≤ 0.05)
Concentration of neurokinin B (NKB) (ng/L) by month of life of singleton (S – n = 13) and twin (T – n = 11) ewes. The values presented in the figure are mean (±SD) concentrations in plasma collected monthly every 15 min for 2 h. * – statistically significant difference between S and T (P-value ≤ 0.05); a, b, c – mean values in a particular month marked with different letters differ significantly (P-value ≤ 0.05)

Fig. 3.

Concentration of dynorphin A (Dyn A) (ng/L) by month of life of singleton (S – n = 13) and twin (T – n = 11) ewes. The values presented in the figure are mean (±SD) concentrations in the plasma collected monthly every 15 min for 2 h. a, b – mean values obtained in a particular month marked with different letters differ significantly (P-value ≤ 0.05)
Concentration of dynorphin A (Dyn A) (ng/L) by month of life of singleton (S – n = 13) and twin (T – n = 11) ewes. The values presented in the figure are mean (±SD) concentrations in the plasma collected monthly every 15 min for 2 h. a, b – mean values obtained in a particular month marked with different letters differ significantly (P-value ≤ 0.05)

Fig. 4.

Changes in plasma kisspeptin-10 (KiSS-10), neurokinin B (NKB) and dynorphin A (Dyn A) concentrations in singleton Polish Uhruska ewes from the 4th to 10th month of life
Changes in plasma kisspeptin-10 (KiSS-10), neurokinin B (NKB) and dynorphin A (Dyn A) concentrations in singleton Polish Uhruska ewes from the 4th to 10th month of life

Fig. 5.

Changes in plasma kisspeptin-10 (KiSS-10), neurokinin B (NKB) and dynorphin A (Dyn A) concentrations in twin Polish Uhruska ewes from the 4th to 10th month of life
Changes in plasma kisspeptin-10 (KiSS-10), neurokinin B (NKB) and dynorphin A (Dyn A) concentrations in twin Polish Uhruska ewes from the 4th to 10th month of life

Mean body weight of singleton and twin Polish Uhruska ewes and differences in that weight at age points up to the 10th month

Age (months)
045678910
Mean body weight ± SD (kg)Singletons5.60 ± 0.632.16 ± 4.634.69 ± 4.135.26 ± 4.237.57 ± 4.237.96 ± 3.939.27 ± 3.840.96 ± 3.9
Twins4.70 ± 0.529.51 ± 5.131.86 ± 3.233.11 ± 2.935.97 ± 2.936.68 ± 3.138.23 ± 2.739.15 ± 2.6
Difference in mean body weight (%) 16.078.248.166.104.263.372.654.42

Pearson correlation coefficients (r) (P ≤ 0_05) between plasma concentrations of KiSS-10, NKB and Dyn A in singleton and twin Polish Uhruska ewes between their 4th and 10th months of life

KiSS-10NKBDyn A
KiSS-100.86–0.55
SingletonsNKB0.86–0.89
DynA–0.55–0.89
KiSS-100.89–0.98
TwinsNKB0.89–0.94
DynA–0.98–0.94
Language: English
Submitted on: Nov 4, 2025
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Accepted on: Feb 23, 2026
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Published on: Mar 2, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 Natalia Szysiak, Urszula Kosior-Korzecka, Monika Greguła-Kania, Krzysztof Patkowski, Mateusz Fila, Andrzej Junkuszew, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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